我们的网站为什么显示成这样？

可能因为您的浏览器不支持样式，您可以更新您的浏览器到最新版本，以获取对此功能的支持，访问下面的网站，获取关于浏览器的信息：

|本期目录/Table of Contents|

UTP减轻大鼠心脏缺血/再灌注损伤的作用

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2010年第5期
页码:
647-649,663
栏目:
基础研究
出版日期:
2010-06-22

文章信息/Info

Title:
Cardioprotective effect of uridine triphosphate in ischemia/reperfusion injury of rat hearts
作者:
樊磊1; 董萍2; 张峰1; 梅其炳1
1.第四军医大学药学系药理学教研室，陕西 西安 710032；2.空军北京95830部队门诊部，北京 100120
Author(s):
FAN Lei1;  DONG Ping2;  ZHANG Feng1;  MEI Qi-bing1
1.Department of Pharmacology, School of Pharmacy, Fourth Military Medical University, Xi’an 710032, Shaanxi, China; 2.Clinic of 95830 troops of air force, PLA, Beijing 100120
关键词:
缺血/再灌注损伤; 心脏; P2受体; 尿苷三磷酸; 大鼠
Keywords:
ischemia/reperfusion injury;  heart;  P2 receptor;  uridine triphosphate
分类号:
G804；R542.2
DOI:
-
文献标识码:
A
摘要:
目的: 探讨P2Y受体激动剂尿苷三磷酸(UTP)对于大鼠心脏缺血/再灌注损伤(I/RI)的延迟性拮抗作用。方法： 24只SD大鼠随机分为4组：实验对照组、UTP组、UTP+苏拉明(suramin,SRM)组及SRM组。所有大鼠尾静脉给药24 h后，建立Langendorff离体心脏灌流模型。平衡10 min后全心停灌，25 min后复灌，持续再灌40 min。观察心脏再灌注前后血流动力学指标及心肌超微结构，记录心脏表面心电图计算心律失常的发生率，收集冠脉流出液，用全自动生化分析仪测量乳酸脱氢酶(LDH)的水平。结果： 复灌后第5 min和25 min时，UTP组的左室发展压(LVDP)、左室压力微分(±dp/dtmax)恢复率均优于实验对照组(P<0.05，P<0.01)；冠脉流出液中LDH的水平明显值降低(P<0.01)；复灌第5~15 min和第25~35min时的心律失常的发生频率均显著下降(P<0.05，P<0.01)；心肌超微结构的损伤减轻。而以SRM与UTP同时作用后，UTP对心脏的保护作用则被取消。SRM组与实验对照组相比各项指标无明显变化。结论： UTP预处理可对心脏I/RI产生延迟性拮抗作用；而P2Y受体拮抗剂SRM可取消这种作用，表明UTP对心脏的保护作用是通过P2Y受体介导的。
Abstract:
AIM: To study the cardioprotective effect uridine triphosphate (UTP) on ischemia/reperfusion injuries of rat hearts and to explore whether this effect of UTP is mediated by P2Y receptor. METHODS: Twenty-four Sprague Dawley (SD) rats were divided into four groups: control group (0.9% sodium chloride i.v.), UTP group (4.4 μg/kg i.v.), UTP and suramin (antagonist of P2Y receptor) group (4.4 μg/kg i.v.+30 μg/kg i.v.) and suramin group (30 μg/kg i.v.). Twenty-four h after drug injection, rat hearts were isolated and perfused with K-H solution by Langendorff system. The hearts were subjected to 25 min ischemia followed by 40 min reperfusion. Hemodynamic indexes were measured and ECG was recorded. The coronary effluent was collected to measure the lactate dehydrogenase (LDH) level. Ultrastructure of myocardium was observed by transmission electron microscope. RESULTS: Twenty-five min after ischemia, significantly better recovery percentage of left ventricular function (P<0.05, P<0.01) and lower LDH level and arrhythmia incidence rate (both P<0.01) were observed in UTP group compared with those in control group. The myocardial ultrastructure of the UTP group was generally normal and the cardioprotective effects of UTP were eliminated by suramin. CONCLUSION: UTP pretreatment protects rat hearts from ischemia/reperfusion injury, which is cancelled by P2Y receptors’ antagonist suramin, indicating that the myocardial protective effect of UTP is mediated by P2Y receptors.

参考文献/References

［1］Burnstock G, Kennedy C. Is there a basis for distinguishing two types of P2-purinocetor?［J］. Gen Pharmacol, 1985, 16(5):433-440.

［2］Yitzhaki S, Shneyvays V, Jacobson KA, et al. Involvement of uracil nucleotides in protection of cardiomyocytes from hypoxic stress［J］. Biochem Pharmacol, 2005, 69(8):1215-1223.

［3］Yitzhaki S, Shainberg A, Cheporko Y, et al. Uridine-5′- triphosphate (UTP) reduces infarct size and improves rat heart function after myocardial infarct［J］. Biochem Pharmacol, 2006, 72(8):949-955.

［4］Quintana M, Kahan T, Hjemdahl P. Pharmacological prevention of reperfusion injury in acutemyocardial infarction. A potential role for adenosine as a therapeutic agent［J］. Am J Cardiovasc Drugs, 2004, 4(3):159-167.

［5］Erlinge D, Harnek J, van Heusden C, et al. Uridine triphosphate (UTP) is released during cardiac ischemia［J］. Intern J Cardiol, 2005, 100(3):427-433.

［6］Kuzmin AI, Gourine AV, Molosh AI, et al. Effects of preconditioning on myocardial interstitial levels of ATP andits catabolites during regional ischemia and reperfusion in the rat［J］. Basic Res Cardiol, 2000, 95(2):127-136.

［7］Yitzhaki S, Hochhauser E, Porat E, et al. Uridine-5′-triphosphate(UTP) maintains cardiac mitochondrial function following chemical and hypoxicstress［J］.J Mol Cell Cardiol, 2007, 43(5):653-662.

［8］ Marber MS, Latchman DS, Walker JM, et al. Cardiac stress protein elevation 24 hour after brief isehemia or heart stress is associate with resisitance to myocardial infarction ［J］. Circulation, 1993, 88(3):1264-1272.

［9］ Carini R, Alchera E, De Cesaris MG, et al. Purinergic P2Y2 receptors promote hepatocyte resistance to hypoxia［J］. J Hepatol, 2006, 45(2):236-245.

［10］Tian ML, Zou Z, Yuan HB, et al. Uridine 5'-triphosphate (UTP) protects against cerebral ischemia reperfusion injury in rats［J］. Neurosci Lett, 2009, 465(1):55-60.

备注/Memo

备注/Memo:
收稿日期：2010-01-29.基金项目：国家自然科学基金项目资助(30570757) 通讯作者：梅其炳，教授，主要从事心血管药理研究Email：qbmei@fmmu.edu.cn 作者简介：樊磊，硕士生Email:xafl519@163.com
更新日期/Last Update: 2010-06-22