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急性应激及美托洛尔干预对山羊心室肌电生理特性的影响

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2010年第6期
页码:
821-823
栏目:
基础研究
出版日期:
2010-08-23

文章信息/Info

Title:
Effect of acute stress and metoprolol on electrophysiological property of ventricular myocardium in goats
作者:
刘建国1; 王玉堂2; 单兆亮2; 时向民2
1.北京军区总医院心肺中心，北京 100700；2.解放军总医院心内科，北京 100853
Author(s):
LIU Jian-guo1;  WANG Yu-tang2;  SHAN Zhao-liang2;  SHI Xiang-min2
1.Department of Cardiac & Pulmonary Center, General Hospital, Beijing Military Area Command, Beijing 100700, China; 2.Department of Cardiology, PLA General Hospital, Beijing 100853, China
关键词:
应激; 跨壁复极离散; 室性心律失常; 山羊
Keywords:
stress;  transmural dispersion of repolarization;  ventricular arrhythmias;  goat
分类号:
R363
DOI:
-
文献标识码:
A
摘要:
目的: 观察急性应激及美托洛尔干预时山羊心室肌电生理特性的改变，探索应激性室性心律失常的机制及预防的方法。方法： 15只雄性山羊随机分为对照组、急性应激组、美托洛尔干预组，以复合刺激诱发急性应激反应。用高效液相色谱法测定血浆肾上腺素和去甲肾上腺素的浓度。自制复合电极同时记录左心室壁三层心肌的单向动作电位(MAP)，并测量MAP90及左室心肌跨壁复极离散值MAPd90。结果： 与对照组相比，急性应激组和美托洛尔干预组山羊血浆肾上腺素、去甲肾上腺素浓度随刺激时间延长进行性升高，各组山羊左室中层心肌MAP90较外层、内层心肌有延长的趋势。急性应激组山羊左室心肌跨壁复极离散值MAPd90显著增加（P<0.01），而美托洛尔干预组则较急性应激组明显减少(P<0.01)。结论： 复合刺激后山羊呈明显应激，左室壁中层心肌细胞电生理特性独特，急性应激使跨壁复极离散显著增加，可能与应激时室性心律失常相关，美托洛尔干预可拮抗其作用。
Abstract:
AIM: To investigate the electrophysiological alteration of ventricular myocardium in goats with acute stress and metoprolol intervention and to explore the mechanism and prophylactic method of stressful ventricular arrhythmia. METHODS: Fifteen male goats were randomly divided into control group, acute stress group and metoprolol group. Complex stimuli were used to induce acute stressful reactions in goats. Plasma epinephrine and norepinephrine concentrations were measured by high-performance liquid chromatography. Monophasic action potential (MAP) of three slices of left ventricular myocardium was recorded with self-made complex electrodes. MAP90 and MAPd90 (transmural dispersion of repolarization) were also measured. RESULTS: Compared with those in control goats, plasma epinephrine and norepinephrine concentrations in acute stress and metoprolol group increased progressively with elongation of the stimulation. MAP90 of left ventricular mid-myocardium displayed an elongating trend in contrast to epimyocardium and endomyocardium in all groups. MAPd90 of left ventricular myocardium significantly increased in acute stress goats (P<0.01). However, MAPd90 of left ventricular myocardium of metoprolol group decreased significantly in contrast with acute stress goats (P<0.01). CONCLUSION: Complex stimuli induced significant acute stress reaction in goats. Myocardium of left ventricular mid-myocardium displays some unique electrophysiological properties. Acute stress significantly increases the transmural dispersion of repolarization, which may be correlated with ventricular arrhythmia. β adrenergic receptor antagonist can counteract the effect.

参考文献/References

［1］Esch T, Stefano GB, Fricchione GL, et al. Stress in cardiovascular diseases［J］. Med Sci Monit, 2002, 8(5):RA93- RA101.

［2］Wittstein IS, Thiemann DR, Lima JA, et al. Neurohumoral features of myocardial stunning due to sudden emotional stress［J］. N Engl J Med, 2005, 352(6):539-548.

［3］Lampert R, Shusterman V, Burg M, et al. Anger-induced T-wave alternans predicts future ventricular arrhythmias in patients with implantable cardioverter-defibrillators ［J］. J Am Coll Cardiol, 2009, 53(9):774-778.

［4］李泱，马杰，肖建民,等. 应用自制复合电极同步记录兔左心室游离壁三层心肌的单向动作电位［J］. 中国心脏起搏与心电生理杂志, 2003, 17(2)：142-145.

［5］Goldstein DS. Catecholamines and stress［J］. Endocrine Regulations, 2003, 37(2):69-80.

［6］Zhang C, Xu D, Li Y, et al. Effect of autonomic nervous system on the transmural dispersion of ventricular repolarization in intact canine［J］. J Huazhong Univ Sci Technolog Med Sci, 2004, 24(1):37-40.

［7］Antzelevitch C. Tpeak-Tend interval as an index of transmural dispersion of repolarization［J］. Eur J Clin Invest, 2001, 31(7):555-557.

[8]Nakajima K, Furukawa Y, Kurogouchi F, et al. Autonomic control of the location and rate of the cardiac pacemaker in the sinoatrial fat pad of parasympathetically denervated dog hearts［J］. J Cardiovasc Electrophysiol, 2002, 13(9):896-901.

[9]Zhou X, Vance FL 4th, Sims AL, et al. Prevention of high incidence of neurally mediated ventricular arrhythmias by afferent nerve stimulation in dogs［J］. Circulation, 2000, 101(7):819-824.

[10]Gupta P, Patel C, Patel H, et al. T(p-e)/QT ratio as an index of arrhythmogenesis［J］. J Electrocardiol, 2008, 41(6):567-574.

[11]Gelzer AR, Koller ML, Otani NF, et al. Dynamic mechanism for initiation of ventricular fibrillation in vivo［J］. Circulation, 2008, 118(11):1123-1129.

备注/Memo

备注/Memo:
收稿日期：2009-09-28.基金项目：全军“十一五”计划课题资助（06G104） 作者简介：刘建国，主治医师，博士Email:liujgdp@sina.com
更新日期/Last Update: 2010-08-22