AGEs对心肌微血管内皮细胞血管新生和管腔形成的影响
《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]
- 期数:
- 2011年第1期
- 页码:
- 42-45
- 栏目:
- 基础研究
- 出版日期:
- 2010-10-27
文章信息/Info
- Title:
- Effect of advanced glycation end products on angiogenesis and tube formation of microvascular endothelial cells
- Author(s):
- SU Yan-hui; LI Fei; LIU Nan; WANG Dong-juan; WANG Hai-chang
- Department of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi, China
- Keywords:
- advanced glycation end products; cardiac microvascular endothelial cell; angiogenesis; tubule-like structure formation
- 分类号:
- R587.2
- DOI:
- -
- 文献标识码:
- A
- 摘要:
- 目的: 观察糖基化终产物（advanced glycation end products，AGEs）对心肌微血管内皮细胞(cardiac microvascular endothelial cells，CMECs)增殖能力、迁移能力，以及血管新生和管腔形成的作用和影响。方法： 以不同浓度（100、200和400 mg/L）的AGEs作用于CMECs 48 h，分别采用MTT比色法检测细胞的增殖能力；用Transwell法检测细胞的迁移能力；用毛细血管管腔结构形成试验检测AGEs对血管新生的影响。结果： CMECs在以不同浓度（100、200及400 mg/L）的AGEs作用48 h后，MTT比色法检测显示，吸光值（分别为0.1195±0.0049、0.1422±0.0058及0.1783±0.0220）明显高于对照组（0.0955±0.0161，P<0.05，P<0.01）；Transwell法检测细胞数［分别为(24.89±6.234)、(32.89±6.990)及(55.56±10.27)个］均高于对照组［（13.89±4.622）个，P<0.05，P<0.01］；管样结构的长度［分别为(3.261±0.7016)、(4.737±1.129)及(6.687±1.308)mm/mm2］均高于对照组［(2.089±0.6723)mm/mm2，P<0.05，P<0.01］。结论： AGEs可以促进CMECs血管新生和管腔结构的形成，且与其浓度呈正相关。
- Abstract:
- AIM: To investigate the effects of the advanced glycation end products (AGEs) on the proliferation, migration and capillary tubule-like formation (TLS) in cardiac microvascular endothelial cells (CMECs). METHODS: CMECs were cultured with AGEs at different concentrations (100 mg/L, 200 mg/L, and 400 mg/L) for 48 h. MTT and transwell were used to detect the proliferation and migration and capillary tubule-like formation was examined to detect the angiogenesis. RESULTS: The A values of CMECs at the AGEs concentration of 100 mg/L, 200 mg/L and 400 mg/L after 48 h incubation were 0.1195±0.0049, 0.1422±0.0058 and 0.1783±0.0220, significantly higher than 0.0955±0.0161 (P<0.05, P<0.01). The migrations of CMECs (24.89±6.234, 32.89±6.990 and 55.56±10.27) were higher than 13.89±4.622 (P<0.05, P<0.01), and the tubule-like formation structures of CMECs were 3.261±0.7016, 4.737±1.129 and 6.687±1.308, higher than 2.089±0.6723 (P<0.05, P<0.01 vs. control group) in a concentration dependent manner. CONCLUSION: AGEs promote the proliferation, migration and tubule-like structure formation in a concentration-dependent manner.
参考文献/References
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备注/Memo
- 备注/Memo:
- 收稿日期：2010-02-08.通讯作者：王海昌，主任医师，主要从事冠心病基础研究 Email：wanghc@fmmu.edu.cn 作者简介：粟妍晖，硕士生Email:shiyao2357@sina.com
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