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|本期目录/Table of Contents|

诱导型环氧合酶-2在CD40配体诱导基质金属蛋白酶表达中的作用

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2011年第1期
页码:
56-58,64
栏目:
基础研究
出版日期:
2010-10-27

文章信息/Info

Title:
rhCD40L induces expression of matrix metalloproteinases in cultured U937 cells by cyclooxygenase-2 pathway
作者:
李岚1; 樊民2; 杨靖2; 汪沁沁2
1.解放军第411医院内4科，上海 200081；2.第二军医大学长征医院心内科，上海 200003
Author(s):
LI Lan1;  FAN Min2;  YANG Jing2;  WANG Qin-qin2
1.Fourth Medical Department, PLA 411 Hospital, Shanghai 200081, China; 2.Department of Cardiovasology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China
关键词:
CD40配体; 诱导型环氧合酶-2; 基质金属蛋白酶; 单核细胞系U937
Keywords:
CD40 ligand;  cyclooxygenase-2;  matrix metalloproteinases;  monocyte
分类号:
Q559
DOI:
-
文献标识码:
A
摘要:
目的: 探讨重组人CD40配体（rhCD40L）诱导U937细胞中基质金属蛋白酶（MMPs）表达的作用及其与诱导型环氧合酶-2(COX-2)途径的关系。方法： 体外培养U937细胞，以不同浓度（0.1、0.2、0.4 mg/L）的rhCD40L和不同浓度（10-5 mol/L、10-4 mol/L、10-3 mol/L）的COX-2特异性抑制剂(NS-398)分别刺激24 h。在加入终浓度为0.4 mg/L rhCD40L的基础上，加入终浓度为10-4 mol/L的NS-398，共同刺激U937细胞24 h后，收集培养上清液，用酶谱法测定MMPs的活性。结果： rhCD40L可使MMP-2和MMP-9的活性明显增加（P<0.01），且呈剂量依赖性；NS-398可抑制MMP-2和MMP-9的活性，且抑制作用随剂量的增加而增强（P<0.05）。结论： rhCD40L以浓度依赖的方式诱导MMPs表达，rhCD40L对MMPs的诱导作用可能与COX-2途径有关。
Abstract:
AIM: To evaluate matrix metalloproteinase (MMP) expression induced by rhCD40L in cultured U937 cell and possible effects of cyclooxygenase-2 (COX-2). METHODS: U937 cells were treated with rhCD40L and NS-398 in different concentrations, respectively, and on the basis of the treatment with rhCD40L, U937 cells were cocultured with NS-398 and aspirin. The supernatant of the U937 cells cultures was collected. Zymography was performed by SDS-PAGE in 10% gels containing 0.1% (w/v) gelatin (denatured collagen) at 4℃. The activities of MMPs were detected by the grey level of the band. RESULTS: rhCD40L increased the activity of MMPs in a dose-dependent manner, whereas NS-398 significantly inhibited the activity of MMPs. NS-398 significantly inhibited the activity of MMPs induced by rhCD40L. CONCLUSION: rhCD40L can induce U937 cells to secrete MMPs in a dose-dependent manner, which may be related to COX-2 pathway.

参考文献/References

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[2]Nagashima H, Aoka Y, Sakomura Y, et al. Matrix metalloproteinase 2 is suppressed by trapidil, a CD40-CD40 ligand pathway inhibitor, in human abdominal aortic aneurysm wall[J]. J Vasc Surg, 2004, 39(2):447-453.

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[7]Rupp J, Berger M, Reiling N, et al. Cox-2 inhibition abrogates Chlamydia pneumoniae-induced PGE2 and MMP-1 expression[J]. Biochem Biophys Res Commun, 2004, 320(3):738-744.

[8]Choi YA, Lee DJ, Lim HK, et al. Interleukin-1beta stimulates matrix metalloproteinase-2 expression via a prostaglandin E2-dependent mechanism in human chondrocytes[J]. Exp Mol Med, 2004, 36(3):226-232.

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[10]Pillinger MH, Rosenthal PB, Tolani SN, et al. Cyclooxygenase-2-derived E prostaglandins down-regulate matrix metalloproteinase-1 expression in fibroblast-like synoviocytes via inhibition of extracellular signal-regulated kinase activation[J]. J Immunol, 2003, 171(11):6080-6089.

备注/Memo

备注/Memo:
收稿日期：2010-01-19.基金项目：上海市卫生局科研课题资助（2007105） 通讯作者：樊民，教授，主要从事冠心病临床及基础研究 Email:fanmin@medmail.com.cn 作者简介：李岚，主治医师，硕士Email：lilan0824@sina.com.cn
更新日期/Last Update: 2010-10-27