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血管紧张素Ⅱ诱导内皮细胞产生IL-8和Duffy抗原/趋化因子受体的研究

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2011年第2期
页码:
173-176
栏目:
基础研究
出版日期:
2010-12-10

文章信息/Info

Title:
Angiotensin II stimulates production of interleukin-8 and Duffy antigen/receptor for chemokine from endothelial cells
作者:
王肖1; 尹文1; 黄杨1; 史小鹏2; 杨天伦3; 周知4; 李传昶3
第四军医大学西京医院：1.急诊科，2.药剂科，陕西 西安 710032；中南大学：3.湘雅医学院心血管内科，4.药学院药理学系，湖南 长沙 410078
Author(s):
WANG Xiao1;  YIN Wen1;  HUANG Yang1;  SHI Xiao-peng2;  YANG Tian-lun3;  ZHOU Zhi4;  LI Chuan-chang3
1.Department of Emergency, 2.Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi, China; 3.Department of Cardiology, Xiangya Hospital, 4.Department of Clinical Pharmacy, Central Southern University, Changsha 410078, Hunan, China
关键词:
Duffy抗原/趋化因子受体; 白介素-8; 血管紧张素Ⅱ; 炎症
Keywords:
Duffy antigen/receptor for chemokine;  interleukin-8;  angiotensin II;  inflammation
分类号:
R972.5
DOI:
-
文献标识码:
A
摘要:
目的: 探讨Duffy抗原/趋化因子受体（DARC）和IL-8在血管紧张素Ⅱ（AngⅡ）致炎机制中可能的作用。方法： 体外培养人脐静脉内皮细胞，分别用PBS（对照组）、AngⅡ（10-7 mol/L）诱导0~24 h，收集0、6、12、24 h4个时间点的细胞培养上清和内皮细胞，采用ELISA法分别检测细胞培养上清和内皮细胞裂解液中IL-8的水平；采用实时定量PCR检测内皮细胞中DARC mRNA的表达。结果： AngⅡ（10-7 mol/L）能诱导内皮细胞产生并释放IL-8，且存在时间依赖性。细胞培养上清中IL-8的水平6~12 h内逐渐增高，24 h后则降低；细胞裂解液中IL-8水平的变化也存在时间依赖性，6~24 h内逐渐增高，其变化与细胞培养上清不平行。AngⅡ可诱导内皮细胞中DARC mRNA表达，也存在时间依赖性，6~24 h逐渐增高。结论： AngⅡ能诱导人内皮细胞产生并释放IL-8和表达DARC。内皮细胞DARC可能通过结合固定IL-8促进趋化因子浓度梯度的形成而参与炎症反应过程。
Abstract:
AIM: To explore the roles of interleukin-8 (IL-8) and Duffy antigen/receptor for chemokine (DARC) in AngII-induced inflammation in endothelial cells. METHODS: Endothelial cells from human umbilical vein (HUVEC) were cultured and stimulated with Ang II (10-7 mol/L) for 0-24 h with phosphate-buffered saline (PBS）as the control. The supernatant and cell lyses of endothelial cells were collected at 0, 6, 12 and 24 h. IL-8 levels were measured by enzyme-linked immunosorbent assay (ELISA). DARC mRNA expression in endothelial cells was measured by real-time polymerase chain reaction (real-time PCR). RESULTS: AngII（10-7mol/L）stimulated IL-8 production in a time-dependent manner. IL-8 level in the supernatant increased gradually during 0-6 h and decreased at 24 h. IL-8 level in cell lyses also increased during 6-24 h in a time-dependent manner, but the changes were not parallel with those in the supernatant. Ang II also stimulated DARC mRNA expression during 6-24 h in a time-dependent manner. CONCLUSION: AngII stimulates IL-8 release and DARC expression from HUVECs. IL-8 and DARC may be involved in the process of AngII-induced inflammation, suggesting that DARC binds IL-8 to form chemokine concentration gradient in inflammation.

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备注/Memo

备注/Memo:
收稿日期：2010-03-07.基金项目：陕西省自然科学基金计划研究项目资助（2009JM4027；2009K14-02） 通讯作者：尹文，主任医师，从事急诊医学领域的基础和临床研究Email:xjyyyw@fmmu.edu.cn 作者简介：王肖，住院医师，硕士Email:wangxiao_29@163.com
更新日期/Last Update: 2010-12-10