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|本期目录/Table of Contents|

胰岛素控制血糖减轻Ⅰ型糖尿病小鼠心肌缺血/再灌注损伤(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2011年第3期
页码:
284-288
栏目:
基础研究
出版日期:
2011-05-12

文章信息/Info

Title:
Glucose control with insulin attenuates myocardial ischemia/reperfusion injury in mice with type 1 diabetes
作者:
王汝涛; 王海昌; 廉坤; 夏陈海; 刘毅; 马新亮; 陶凌
第四军医大学西京医院心血管内科，陕西 西安710032
Author(s):
WANG Ru-tao;  WANG Hai-chang;  LIAN Kun;  XIA Chen-hai;  LIU Yi;  MA Xin-liang;  TAO Ling
Department of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shanxi, China
关键词:
胰岛素; 糖尿病; 缺血/再灌注损伤; 小鼠
Keywords:
insulin;  glucose control;  diabetes;  ischemia/reperfusion injury;  mice
分类号:
R587.1
DOI:
-
文献标识码:
A
摘要:
目的:研究Ⅰ型糖尿病(DM)小鼠心肌缺血/再灌注(I/R)损伤的变化，以及胰岛素(ISL)治疗控制血糖对Ⅰ型DM小鼠心肌I/R损伤的影响。方法: 72只健康雄性昆明小鼠以链脲佐菌素(STZ)腹腔注射制备Ⅰ型DM模型，建模后用ISL治疗控制血糖2周。实验分为假手术（sham）组、I/R组、DM+I/R组、DM+ISL+I/R组，每组18只。采用结扎冠状动脉左前降支30 min制备心肌梗死(MI)模型，分别于再灌注后3 h，用TUNEL法检测心肌细胞凋亡，用ELISA法检测caspase-3活性、硝基酪氨酸的含量。再灌注后24 h，用TTC染色法检测MI面积。结果: 与I/R组相比，DM+I/R组MI面积增大、心肌细胞凋亡数量增加、caspase-3活性升高、硝基酪氨酸的含量增加（P<0.01）。与DM+I/R组相比，DM+ISL+I/R组心肌细胞凋亡数量减少（P<0.01），MI面积减少、caspase-3活性降低、硝基酪氨酸含量减少（P<0.05）。结论: Ⅰ型DM小鼠心肌I/R损伤增加，ISL治疗控制血糖可以减轻其心肌I/R损伤。
Abstract:
AIM:To investigate the effect of glucose control with insulin on myocardial ischemia/reperfusion (MI/R) in streptozotocin (STZ)-induced diabetic mice. METHODS: Type 1 diabetes was induced by i.p. injection of STZ and was treated with insulin for 2 weeks. Mice were divided into four groups: sham group, I/R group, DM+I/R group, and DM+insulin+I/R group. Myocardial ischemia was produced by temporarily exteriorizing the heart via a left thoracic incision and placing a 6-0 silk suture slipknot around the left anterior descending coronary artery. After 30 min of ischemia, the slipknot was released and the myocardium was reperfused for 3 h (for caspase-3 activity and nitrotyrosine contents by ELISA, apoptosis by TUNEL) or 24 h (for infarct size by TTC staining). RESULTS: Compared with those in I/R group, infarct size, myocardial apoptosis, caspase-3 activity and nitrotyrosine contents increased in DM+I/R group (P<0.01). Compared with those in DM+I/R group, myocardial apoptosis (P<0.01), infarct size, caspase-3 activity and nitrotyrosine contents (P<0.05) were reduced in DM+insulin+I/R group. CONCLUSION: Type 1 diabetes aggravates MI/R injury and glucose control with insulin attenuates MI/R injury in STZ-induced diabetic mice.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2010-06-07.基金项目：国家自然科学基金项目资助（30670879）； 西京医院学科助推计划项目资助（Xjzt08z02） 通讯作者：王海昌，主任医师，主要从事冠心病的研究Email:wanghc@fmmu.edu.cn 共同通讯作者：陶凌，主任医师,主要从事糖尿病心肌缺血再灌注损伤的研究Email:lingtao2006@gmail.com 作者简介：王汝涛，硕士生Email:wang.rutao1226@gmail.com
更新日期/Last Update: 2011-03-17