我们的网站为什么显示成这样？

可能因为您的浏览器不支持样式，您可以更新您的浏览器到最新版本，以获取对此功能的支持，访问下面的网站，获取关于浏览器的信息：

|本期目录/Table of Contents|

血管紧张素转化酶抑制剂和抗氧化剂对湿热应激性大鼠心肌AngⅡ和p22phox表达的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2011年第3期
页码:
300-303，308
栏目:
基础研究
出版日期:
2011-05-12

文章信息/Info

Title:
Effect of angiotensin-converting enzyme inhibitors and antioxidants on heat stress-induced expression of rat p22phox
作者:
侯小玲1; 赵连友2; 陈韵岱1
1.解放军总医院心血管内科，北京 100853；2.第四军医大学唐都医院心血管内科，陕西 西安 710032
Author(s):
HOU Xiao-ling1;  ZHAO Lian-you2;  CHEN Yun-dai1
1.Department of Cardiology, PLA General Hospital, Beijing 100853, China; 2.Department of Cardiology, Tangdu Hospital, Fourth Military Medical University, Xi’an 710038, Shaanxi, China
关键词:
血管紧张素转化酶抑制剂; 抗氧化剂; 热应激; 血管紧张素Ⅱ; p22phox; 大鼠
Keywords:
heat stress;  angiotensin II;  reactive oxygen species;  p22phox
分类号:
R852.51
DOI:
-
文献标识码:
A
摘要:
目的:观察血管紧张素转化酶抑制剂(ACEI)卡托普利(Captopril,CTP)和抗氧化剂（维生素C，VitC）对湿热应激(HHS)大鼠心肌组织中血管紧张素Ⅱ（AngⅡ)、活性氧簇（ROS）和p22phox表达的影响。方法： 将32只成年雄性SD大鼠随机分为：对照组、HHS组、CTP组(HHS+CTP)及VitC组(HHS+VitC)，每组8只。喂养4周后，颈动脉插管测定大鼠血压，计算大鼠左心室质量指数。用放射免疫法测定心肌组织中AngⅡ的浓度。用比色法测定心肌组织中ROS的水平。应用RT-PCR检测p22phox mRNA的表达。用免疫组化染色法检测大鼠心肌中p22phox的分布特征。结果： 平均动脉压、AngⅡ和ROS和p22phox的水平，HHS组与对照组比较，VitC组和CTP组与HHS组比较，均有非常显著性差异（P<0.01）；两个药物组之间比较无统计学意义。结论: HHS可增加大鼠心肌组织中AngⅡ表达，同时上调p22phox mRNA和其蛋白的表达，介导心肌细胞内ROS生成增加。用抗氧化剂和ACEI阻滞后，AngⅡ表达减少，p22phox mRNA和其蛋白的表达降低，心肌细胞内ROS生成减少，其机制可能与HHS导致AngⅡ诱导ROS产生有关。以上提示，HHS可引起心脏损害，抗氧化剂和ACEI对HHS性心脏损害具有拮抗作用。
Abstract:
AIM:To study the effect of heat stress on the expression of reactive oxygen species（ROS）, p22phox and angiotensin II (AngII) in myocardium and to investigate the interrelation among ROS, p22phox and AngII in myocardium. METHODS: Twenty-four Sprague Dawley rats were divided randomly into three groups: control group, high temperature and high humidity stress group and heat stress group. All examinations were performed 4 weeks later. Mean arterial blood pressure (MABP) was measured by carotid artery intubation and ROS was determined with spectrophotometer. Concentration of AngII in plasma and myocardium was detected, respectively, by radioimmunoassay. Expression level of p22phox mRNA in myocardium was determined by reverse transcription polymerase chain reaction (RT-PCR) and p22phox protein was detected by immunohistochemistry and semiquantitative analysis in different groups. RESULTS: Compared with control group, MBP, ROS, p22phox mRNA and p22phox protein levels in high temperature and high humidity stress group and heat stress group significantly increased (P<0.01). No significant difference was observed in LVW/BW index. The concentration of AngII in plasma and myocardium increased markedly. CONCLUSION: The concentration of AngII in plasma and myocardium as well as p22phox in the myocardium increases under heat stress conditions. Heat stress may be associated with overproduction of ROS, possibly by increased expression of AngII.

参考文献/References

[1]何丽华,张金良,张颖,等.高温对大鼠血压及血管活性物质的影响[J].北京大学学报,2005,37(4):448-449.
[2]Finnoff JT.Environmental effects on brain function[J].Curr Sports Med Rep,2008,7(1):28-32.
[3]Zhang HJ,Doctrow SR,Xu L,et al.Redox modulation of the liver with chronic antioxidant enzyme mimetic treatment prevents age-related oxidative damage associated with environmental stress[J].FASEB J,2004,18(13):1547-1549.
[4]Zuo L,Christofi FL, Wright VP,et al.Intra and extracellular measurement of reactive oxygen species produced during heat stress in diaphragm muscle[J].Am J Physiol Cell Physiol,2000,279(4):C1058-C1066.
[5]孙永建,王前,裴国献,等.热应激预处理对大鼠肢体缺血再灌注后血清丙二醛、超氧化物歧化酶的影响[J].第一军医大学学报,2002,22(6):506-508.
[6]Oroge W.Oxidative stress and aging[J].Adv Exp Med Biol,2003,543:191-200.
[7]Shringarpure R,Davies KJ.Protein turnover by the proteasome in aging and disease[J].Free Radic Biol Med,2002,32(11):1084-1089.
[7]Marciniak SJ,Ron D.Endoplasmic reticulum stress signaling in disease[J].Physiol Rev,2006,86(4):1133-1149.
[8]Li JM,Gall NP,Grieve DJ,et al. Activation of NADPH oxidase during progression of cardiac hypertrophy to failure[J].Hypertension, 2002,40(4):477-484.
[9]Lassegue B,Griendling KK.Reactive oxygen species in hypertension:an update[J].Am J Hypertens,2004,17(9):852-860.
[10]Touyz RM,Tabet F,Schiffrin EL.Redox-dependent signalling by angiotensin II and vascular remodelling in hypertension［J］. Clin Exp Pharmacol Physiol,2003,30(11):860-866.
[11]侯小玲,赵连友,薛玉生,等.高温应激对大鼠心肌血管紧张素Ⅱ的影响及其与心肌p22phox表达的关系[J].心脏杂志,2009,21(3):304-308.
[12]Griendling KK,Sorescu D,Ushio-Fukai M.NAD(P)H oxidase role in cardiovascular biology and disease[J].Circ Res,2000,86(5):494-501.
[13]Hitomi H,Kiyomoto H,Nishiyama A.Angiotensin II and oxidative stress［J］. Curr Opin Cardiol, 2007, 22(4):311-315.
[14]Griendling KK, Sorescu D, Lassègue B,et al.Modulation of protein kinase activity and gene expression by reactive oxygen species and their role in vascular physiology and pathophysiology[J].Arterioscler Thromb Vasc Biol,2000, 20(10):2175-2183.
[15]Cracowski JL,Stanke-Labesque F,Souvignet C,et al.Isoprostanes:new markers of oxidative stress in human diseases[J].Presse Med,2000,29(11):604-610.
[16]Sata M,Fukuda D.Crucial role of renin-angiotensin system in the pathogenesis of atherosclerosis[J].J Med Invest,2010,57(1-2):12-25.

备注/Memo

备注/Memo:
收稿日期：2010-06-01.通讯作者：赵连友，主任医师，主要从事高血压发病机制及相关疾病防治研究 Email：zhaolyfmmu@yahoo.com.cn 作者简介：侯小玲，住院医师，硕士Email：houxiaoling.good@163.com
更新日期/Last Update: 2011-03-17