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|本期目录/Table of Contents|

男性冠心病患者介入治疗前后外周血平滑肌前体细胞数量的变化(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2011年第3期
页码:
347-350
栏目:
临床研究
出版日期:
2011-05-12

文章信息/Info

Title:
Changes of circulating smooth muscle progenitor cells in male patients with coronary heart disease before and after percutaneous coronary intervention
作者:
倪旭东; 周学凯; 李飞; 张荣庆; 郭文怡
第四军医大学西京医院心脏内科，陕西 西安 710032
Author(s):
NI Xu-dong;  ZHOU Xue-kai;  LI Fei;  ZHANG Rong-qing;  GUO Wen-yi
Department of Cardiovascular Medicine, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi, China
关键词:
干细胞; 冠状动脉疾病; 冠状动脉血管成形术; 经腔; 经皮; 平滑肌前体细胞
Keywords:
stem cells;  coronary disease;  coronary angioplasty;  transluminal;  percutaneous;  smooth muscle progenitor cells
分类号:
R541.4
DOI:
-
文献标识码:
A
摘要:
目的:观察比较正常人与冠心病患者外周血平滑肌前体细胞（SPCs）数量的差异，以及冠心病患者在经皮冠脉介入治疗（PCI）前后外周血中SPCs数量的变化。方法： 将研究对象分为3组：稳定型心绞痛（SAP）组、不稳定型心绞痛(UAP)及正常对照组(NC)。SPCs以CD14和CD105双阳性确定，利用流式细胞仪双色分析技术筛选各组在PCI术前、术后即刻、术后72 h SPCs占外周血有核细胞的百分比。结果： PCI术前，外周血中SPCs数量在SAP组为（0.20±0.13）%、UAP组为（0.28±0.18）%，均明显高于NC组［（0.12±0.10）%］（均P<0.01），UAP组外周血中SPCs数量明显高于SAP组（P<0.01）。UAP组外周血中SPCs的数量在PCI术后72 h较术前明显增加［（0.34±0.25）% vs. （0.28±0.18）%，P<0.01］。结论： 外周血中SPCs数量的变化及PCI对外周血SPCs数量的影响可能与冠心病的临床类型有关。
Abstract:
AIM:To compare the number of circulating smooth muscle progenitor cells (SPCs) between normal controls and patients with coronary heart disease (CHD) and to explore the influence of percutaneous coronary (PCI) on the SPC number in CHD patients. METHODS: A total of 33 hospitalized male CHD patients were enrolled and divided into three groups, including stable angina pectoris (SAP) group and unstable angina pectoris (UA) group. Patients with normal coronary angiography served as controls. The percentage of SPCs in peripheral blood mononuclear cells (PBMCs) was measured at admission and 72 h after PCI in CHD patients by double-color flow cytometry analysis. SPCs were identified with CD14+/CD105+. RESULTS: At admission, the percentage of SPCs in PBMCs was significantly higher in SAP group (0.20±0.13)% and UA group (0.28±0.18)% than in control group ［(0.12±0.10)%, all P<0.01］. The number of SPCs in UA group was significantly higher than in the SAP group (P<0.01). In the UA group, the number of SPCs at 72 h after PCI (0.34±0.25)% was significantly higher than before operation ［(0.28±0.18)%, P<0.01］ and tended to be higher than the value immediately after PCI. CONCLUSION: Stent implantation may induce the mobilization of bone marrow-derived smooth muscle progenitor cells. The number of peripheral SPCs in CHD patients is lower than in normal subjects and is negatively related with the severity of coronary heart disease. The number of circulating SPCs increases post-PCI in patients with UA.

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备注/Memo

备注/Memo:
收稿日期：2010-06-08.通讯作者：郭文怡，主任医师，主要从事冠心病介入治疗研究Email:wenyiguo@yahoo.com 作者简介：倪旭东，主治医师，硕士生Email:dzn229@163.com
更新日期/Last Update: 2011-03-17