我们的网站为什么显示成这样？

可能因为您的浏览器不支持样式，您可以更新您的浏览器到最新版本，以获取对此功能的支持，访问下面的网站，获取关于浏览器的信息：

|本期目录/Table of Contents|

自发性高血压大鼠发病过程中结缔组织生长因子表达的动态观察(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2011年第4期
页码:
446
栏目:
基础研究
出版日期:
2011-08-25

文章信息/Info

Title:
Dynamic observation of connective tissue growth factor in pathogenic process of spontaneously hypertensive rats
作者:
张颖1; 牛小麟2; 尚亮1
1.陕西省人民医院西院一病区,陕西 西安 710068；2.西安交通大学第二附属医院心血管内科，陕西 西安 710004
Author(s):
ZHANG Ying1;  NIU Xiao-lin2;  SHANG Liang1
1.First Patient’s District, West Yard of Shaanxi Provincial Peoples Hospital, Xi’an 710068, Shaanxi, China; 2.Department of Cardiovasology, Second Affiliated Hospital, Medical College, Xi’an Jiaotong University, Xi’an 710004, Shaanxi, China
关键词:
结缔组织生长因子; 自发性高血压; 心肌细胞; 大鼠
Keywords:
connective tissue growth factor;  spontaneously hypertensive rats;  cardiomyocyte;  rat
分类号:
R544.1
DOI:
61-1268/R.20110503.1402.007
文献标识码:
A
摘要:
目的:观察自发性高血压大鼠(SHR)发病过程中结缔组织因子(CTGF)的动态变化，探讨其与高血压病发生发展的关系。方法： 采用RT-PCR检测心肌组织中CTGF mRNA的表达。用苏木精、伊红染色检测心肌纤维化。用免疫组化染色(SP法)检测CTGF在心肌组织中的分布。结果： 随着鼠龄的增长,SHR组大鼠左室质量指数、CTGF及其mRNA的表达均显著增加，其心肌细胞的大小也明显增大（P<0.05）。SHR组大鼠心肌间质和血管平滑肌中，可见大量的呈棕黄色的CTGF阳性颗粒的分布，不同鼠龄组之间比较亦有显著性差异(P<0.01)。结论： 不同鼠龄(8~16周)的SHR大鼠心肌细胞中CTGF的表达显著增加，尤以16周龄的大鼠为著，同时伴有心肌纤维化进程加快，提示CTGF参与了高血压大鼠心肌病变的发生发展。
Abstract:
AIM:o observe the dynamic changes of connective tissue growth factor (CTGF) in the pathogenic process of spontaneously hypertensive rats (SHRs) and to explore the relationship between CTGF and the development of hypertensive cardiomyopathy. METHODS: RT-PCR was used to detect the expression of CTGF mRNA in myocardial tissues. Myocardial fibrosis was detected by hematoxylin and eosin staining and the distribution of CTGF in myocardial tissues was detected by immunohistochemical staining. RESULTS: With the increase in age, left ventricular weight index, CTGF and its mRNA expression significantly increased in SHR group. The size of cardiac myocytes also increased significantly in SHR group (P<0.05). In the myocardial interstitial and vascular smooth muscle of the SHR group, a large number of brown CTGF positive particles were seen and the number was statistically significant between different age groups (P<0.01). CONCLUSION: The CTGF expression in the myocardial tissue significantly increased in different age group of SHR (from 8 weeks to 16 weeks), especially in the 16-week age group. Meanwhile, myocardial fibrosis progresses, suggesting that CTGF is involved in the occurrence and development of myocardial disease in hypertensive rats.

参考文献/References

[1]Ohnishi H,Oka T,Kusachi S,et al.Increased expression of connective tissue growth factor in the infarct zone of experimentally induced myocardial infarction in rats[J].J Mol Cell Cardio,1998, 130(11):2411-2422.
[2]Finckenberg P,Inkinen K,Ahonen J,et al.Angiotensin Ⅱ induces connective tissue growth factor gene expression via calcineurin-depen- dent pathways[J].Am J Pathol,2003,163(1):355-466.
[3]Hishikawa K,Oemar BS,Nakaki T.Static p ressure regulates con- nective tissue growth factor exp ression in human mesangial cells[J].J Biol Chem,2001,276(20):16797-16803.
[4]Peng H,Carretero OA,Brigstock DR,et al.Ac-SDKP reverses cardiac fibrosis in rats with renovascular hypertension[J].Hypertension,2003,42(6):1164-1170.
[5]Finckenberg P,Inkinen K,Ahonen J,et al.Angiotensin Ⅱ induces connective tissue growth factor gene expression via calcineurin-depen- dent pathways[J].Am J Pathol,2003,163(1):355-466.
[6]Caqalinec M,Kyselovic J,Blaskova E,et al.Comparative study of the effects of lacidipine and enalapril on the left ventricular cardiomyocyte remodeling in spontaneously hypertensive rats[J].J Cardiovasc pharmacol,2006,47(4):561-570.
[7]刘慧,赵连友,郑强荪,等.结缔组织生长因子诱导大鼠心肌细胞肥大的作用及其与细胞外信号调节激酶1/2的关系[J].中华高血压杂志,2006,14(12):984-988.
[8]Li Z,Bing OH,Long X,et al.Increased cardiomyocyte apoptosis during the transition to heart failure in the spontaneously hypertensive rat[J].Am J Physiol,1997, 272(5 Pt 2):H2313-H2319.
[9]Moreau P,Tea BS,Dam TV,et al.Altered balance between cell replication and apoptosis in hearts and kidneys of newborn SHR[J].Hypertension,1997,30(3 Pt 2):720-724.
[10]Hamet P,Moreau P,Dam TV,et al.The time windowof apoptosis:a new component in the therapeutic strategyfor cardiovascular remodeling[J].J Hypertens Suppl,1996,14(Suppl 5):S65-S70.

备注/Memo

备注/Memo:
收稿日期：2010-06-15.基金项目:陕西省科学技术研究发展计划项目［2006K13-G2(2)］ 通讯作者:张颖,副主任医师,主要从事心脑血管病诊治的研究Email:wjf19720125@163.com
更新日期/Last Update: 2011-06-02