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不同降压药物对自发性高血压大鼠模型左室心肌细胞中TRPC3及TRPC6表达的影响 (PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2011年第4期
页码:
459
栏目:
基础研究
出版日期:
2011-08-25

文章信息/Info

Title:
Effects of antihypertensive drugs on expression of TRPC3 and TRPC6 in left ventricle of spontaneously hypertensive rats
作者:
余冬梅; 陈明; 廖雪艳
重庆医科大学附属第一医院心内科，重庆 400016
Author(s):
YU Dong-mei;  CHEN Ming;  LIAO Xue-yan
Department of Cardiology, First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China
关键词:
缬沙坦; 雷米普利; 氨氯地平; 瞬时感受器阳离子通道C亚族; 左室肥厚; 大鼠
Keywords:
valsartan;  ramipril;  anlodipine;  canonical transient receptor potential channel;  left ventricular hypertrophy
分类号:
R544.1　
DOI:
61-1268/R.20110503.1427.010
文献标识码:
A
摘要:
目的:探讨缬沙坦、雷米普利及氨氯地平对自发性高血压大鼠（SHR）左室心肌中瞬时受体通道蛋白C亚族3及6(TRPC3及TRPC6)表达的影响。方法： 将24只12周龄SHR大鼠随机分为4组，即SHR组、缬沙坦组、雷米普利组及氨氯地平组，每组6只。另以6只同龄的Wistar Kyoto大鼠(WKY)为正常对照组。给药4周后，检测各组大鼠的血压、左室质量指数、左室心肌细胞横径；RT-PCR及Western Blot检测TRPC3及TRPC6 mRNA 及其蛋白的表达。结果： SHR组血压、左室质量指数及左室心肌细胞横径均明显高于对照组（P<0.05），3个药物组上述指标均较SHR组降低（P<0.05）；5个组均有TRPC3及TRPC6的表达，SHR组TRPC3及TRPC6 mRNA及其蛋白的表达显著高于对照组（P<0.05），3个药物组TRPC3 mRNA及TRPC6 mRNA及其蛋白的表达均显著低于SHR组（P<0.05），缬沙坦组TRPC3 mRNA及其蛋白表达的减少最显著（P<0.05）；3个药物组TRPC6 mRNA及其蛋白的表达有所下降，但组间比较差异无显著性。结论： SHR组及对照组大鼠均有TRPC3及TRPC6的表达，TRPC3及TRPC6可能共同参与调节心肌肥厚的病理生理过程；缬沙坦可能通过抑制TRPC3蛋白的表达参与逆转左室肥厚的过程。
Abstract:
AIM:To investigate the effect of valsartan, ramipril and anlodipine on the expression of TRPC3 and TRPC6 in the left ventricle in spontaneously hypertensive rats (SHR). METHODS: Twelve-week-old SHRs were randomly divided into four groups: valsartan-treated group (Val), ramipril-treated group (Ram), amlodipine-treated group (Aml) and untreated group (SHR). Twelve-week-old Wistar-Kyoto (WKY) rats were used as control group. After 4 weeks of drug intervention, blood pressure (BP), left ventricular mass index (LVMI), left ventricular cardiomyocytes transection diameter (LVTDM) were examined, RT-PCR and Western blotting were used to detect the expressions of TRPC3 and TRPC6 mRNA and protein. RESULTS: Compared with those in WKY, BP, LVMI and LVTDM in SHR were significantly higher (P<0.05) and the data mentioned above decreased significantly in the drug intervention groups (P<0.05). Expressions of TRPC3 and TRPC6 mRNA and protein were detected in each group and were significantly higher compared with those in WKY. TRPC3 decreased dramatically in drug intervention groups (P<0.05) and TRPC3 mRNA and protein decreased significantly in Val group compared with those in Ram group and Aml group. TRPC6 mRNA and protein expressions decreased in drug intervention groups; however, no significant changes in TRPC6 mRNA and protein expressions were observed. CONCLUSION: Expressions of TRPC3 and TRPC6 were found in SHR and WKY. TRPC3 and TRPC6 may co-regulate the pathphysiological processes of myocardial hypertrophy. Valsartan may inhibit left ventricular hypertrophy, probably by downregulating mRNA and protein expression of TRPC3.

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备注/Memo

备注/Memo:
收稿日期：2010-10-18.通讯作者：陈明，教授，主要研究高血压的发病机制及治疗Email:chenmingcq@126.com 作者简介：余冬梅，硕士生Email:maomao_u@126.com
更新日期/Last Update: 2011-06-02