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|本期目录/Table of Contents|

以野百合碱建立兔慢性肺动脉高压模型的效果分析(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2011年第4期
页码:
465
栏目:
基础研究
出版日期:
2011-08-25

文章信息/Info

Title:
Analyses of monocrotaline-induced rabbit model of chronic pulmonary hypertension
作者:
孙丹丹1; 段云友1 ; 陈洪茂1; 王跃民2; 梁宁南1; 王宇1; 熊华强1
第四军医大学：1.唐都医院超声科,陕西 西安 710038，2.基础部生理学教研室，陕西 西安 710032
Author(s):
SUN Dan-dan1;  DUAN Yun-you1;  CHEN Hong-mao1;  WANG Yue-min2;  LIANG Ning-nan1;  WANG Yu1;  XIONG Hua-qiang1
1.Department of Ultrasound Diagnostics, Tangdu Hospital, 2.Department of Physiology, Fourth Military Medical University, Xi’an 710038, Shaanxi, China
关键词:
野百合碱; 慢性肺动脉高压; 动物模型; 兔
Keywords:
ultrasonography;  pulmonary hypertension;  animal model;  rabbit
分类号:
R543.2
DOI:
61-1268/R.20110503.1429.011
文献标识码:
A
摘要:
目的:探讨野百合碱(MCT)腹腔注射建立新西兰兔慢性肺动脉高压(CPH)模型的可行性。方法： 将84只新西兰兔随机分为两组，即对照组（24只）和模型组（60只）。在相同的饲料和饲养条件下，模型组腹腔注射MCT溶液60 mg/kg，对照组注射相同剂量的乙醇与生理盐水混合液，28 d后进行右心导管压力测定肺动脉的压力。结果： 右心导管各心腔压力的测定值显示，模型组肺的动脉收缩压、舒张压，右室收缩压、舒张压及右房收缩压、舒张压与对照组比较均呈升高的趋势，但仅右房舒张压的差异具有统计学意义（P<0.01）；模型组的平均肺动脉收缩压未达到30 mmHg，说明模型的制备不理想。病理检测的结果显示，与对照组比较，模型组兔肺小动脉血管的管壁增厚，平滑肌组织增殖明显。结论： MCT注射可致兔肺动脉血管平滑肌层增厚，造成血流动力学改变，但所致肺动脉压力升高的结果并不稳定，仍需进一步研究。
Abstract:
AIM:To explore the feasibility of a rabbit model with chronic monocrotaline (MCT)-induced pulmonary hypertension. METHODS: Eighty-four New Zealand rabbits were randomly divided into two groups: control group of 24 rabbits and MCT group of 60 rabbits. The rats in both groups were given the same diet and were raised under the same conditions. Chronic pulmonary hypertension in the MCT group was developed by injection of MCT solution into the abdominal cavity, whereas only the same dosage of the mixed pure ethanol and physiologic saline was injected into the abdominal cavity in the control group. The pulmonary artery systolic pressure was detected by pressure-guided microcatheter into the pulmonary artery 28 days after injection. RESULTS: The results of the right heart catheterization showed that, except for the right atrial diastolic pressure, the pressures of the heart chambers in the MCT group were not significantly different from those in the control group. The average pulmonary artery systolic pressure in the MCT group failed to reach 30 mmHg, and the models were thus not successful. Pathology results showed that the pulmonary artery wall in the MCT group thickened and the smooth muscle tissue significantly proliferated. CONCLUSION: MCT injection can thicken the pulmonary artery smooth muscle in rabbits, suggesting some hemodynamic changes. Increase of pulmonary arterial pressure in rabbits is not stable, necessitating further study in this animal model.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2010-10-18.基金项目:国家自然科学基金项目资助（30772057） 通讯作者:段云友,主任医师，主要从事超声心动图应用研究Email:duanyy@fmmu.edu.cn 作者简介:孙丹丹,主治医师，博士Email:suntuzi@163.com
更新日期/Last Update: 2011-06-02