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CREG在动脉粥样硬化血管中表达的变化(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2011年第5期
页码:
565-569
栏目:
基础研究
出版日期:
2011-10-25

文章信息/Info

Title:
Expression of cellular repressor of E1A-stimulated genes in atherosclerotic artery
作者:
杨桂棠; 张 娜; 段 岩; 游 洋; 康 建; 闫承慧; 韩雅玲
沈阳军区总医院心内科，辽宁 沈阳 110840
Author(s):
YANG Gui-tang;  ZHANG Na;  DUAN Yan;  YOU Yang;  KANG Jian;  YAN Cheng-hui;  HAN Ya-ling
Department of Cardiology, Shenyang Northern Hospital, Shenyang 110840, Liaoning, China
关键词:
动脉粥样硬化; E1A激活基因阻遏子; 血管平滑肌细胞; 兔
Keywords:
atherosclerosis;  E1A-stimulated genes;  repressor;  vascular smooth muscle cells;  rabbit
分类号:
R543.5
DOI:
-
文献标识码:
A
摘要:
目的:探讨E1A激活基因阻遏子（CREG）在动脉粥样硬化(AS)发展中的作用。方法： 采用Western blot方法检测AS患者血管中CREG表达的变化。用高脂饲料喂养兔子的方法模拟AS的自热进程，观察血管的形态学变化。用免疫组化染色法检测血管平滑肌细胞(VSMCs)增殖及CREG、SMα-actin和SM MHC表达的变化。结果： 在AS患者的血管中，CREG的表达明显下调。在动物模型中，以高脂饲料喂养兔1个月时，血管内皮出现不光滑、内膜增厚，中膜VSMCs出现表型转化，收缩蛋白SMα-actin和SM MHC的表达开始下降，细胞出现增生，此时CREG的表达也开始下调。以高脂饲料喂养兔2个月时，可见内皮有中断、破损现象，内膜增厚明显，增殖细胞核抗原(PCNA)表达阳性的细胞明显增多，CREG的表达进一步下调，VSMCs中的收缩蛋白也进一步下降。结论： 在AS进程中，血管中膜的VSMCs由收缩表型向合成表型转化，细胞增生活跃、分化减弱，CREG随着AS的发展，在VSMCs中的表达逐步下降，提示CREG作为维持VSMCs分化的蛋白与AS的发生发展密切相关。
Abstract:
AIM:To investigate the effects of cellular repressor of E1A-stimulated genes (CREG) on atherosclerotic lesion formation. METHODS: Western blot was used to detect the expression of CREG in human normal and atherosclerotic arteries from amputation surgery. The morphology of the artery and expression of CREG, smooth muscle α-actin （SMα-actin）and smooth muscle-myosin heavy chain (SM-MHC) on natural atherosclerotic formation were observed in rabbits fed a high-fat diet. RESULTS: In human atherosclerotic artery, the expression of CREG decreased significantly in atherosclerotic artery, accompanied by the decrease of SMα-actin. In rabbit models fed a high-fat diet for 1 month, the endothelium was not smooth and the intima became thicker. The phenotype of vascular smooth muscle cells (VSMCs) changed. Expression of CREG, SMα-actin and SM-MHC decreased and VSMCs proliferated. In rabbit models fed a high-fat diet for 2 months, the endothelium discontinued and the intima became thicker. The number of proliferating cell nuclear antigen (PCNA) positive cells was much higher and the levels of CREG, SMα-actin and SM-MHC protein were lower in VSMCs. CONCLUSION: The phenotype of VSMCs changes from differentiation to synthesis in atherosclerotic formation. CREG expression decreases with the development of atherosclerosis, suggesting that CREG is associated with atherosclerosis.

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备注/Memo

备注/Memo:
收稿日期：2010-11-02.基金项目：国家自然科学基金项目资助(30770793，30971218) 通讯作者：韩雅玲，教授，主要从事冠心病的诊断与治疗的研究Email:hanyal@mail.sy.ln.cn 作者简介：杨桂棠，主治医师，博士生Email:yanggt1104@sina.com
更新日期/Last Update: 2011-11-03