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α-亚麻酸对糖尿病大鼠血管功能和氧化应激的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2011年第5期
页码:
575-578
栏目:
基础研究
出版日期:
2011-10-25

文章信息/Info

Title:
Effects of alpha-linolenic acid on vascular function and oxidative stress in diabetic rats
作者:
张 薇1; 奚苗苗2; 李 波1; 刘 军1; 牛晓琳1; 张利华1
第四军医大学：1.唐都医院心血管内科，2.西京医院药剂科，陕西 西安 710032
Author(s):
ZHANG Wei1;  XI Miao-miao2;  LI Bo1;  LIU Jun1;  NIU Xiao-lin1;  ZHANG Li-hua1
1.Department of Cardiology, Tangdu Hospital, 2.Department Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi, China
关键词:
α-亚麻酸; 糖尿病; 血管功能; 氧化应激; 大鼠
Keywords:
alpha-linolenic acid;  diabetes;  vascular function;  oxidative stress
分类号:
R587.1
DOI:
-
文献标识码:
A
摘要:
目的:观察α-亚麻酸（ALA）对糖尿病(DM)大鼠血管功能和氧化应激的影响，探讨ALA在DM血管并发症防治中的作用。方法： 将30只SD大鼠随机分为正常对照组、DM模型组和ALA治疗组［500 μg/(kg·d)］，每组10只。10只雄性SD大鼠以高脂饮食喂养4周后，腹腔注射链脲佐菌素（STZ）30 mg/kg建立Ⅱ型DM（T2DM）模型。4周后分离降主动脉，进行离体血管灌流观察血管的舒张功能，利用试剂盒测定灌流液中NO的含量以及血管组织中丙二醛（MDA）的含量、超氧化物岐化酶（SOD）和过氧化氢酶（CAT）的活性。结果： 与正常对照组相比，DM大鼠主动脉内皮依赖性舒张功能和NO的含量显著下降(P<0.01)，ALA治疗可有效地减轻DM大鼠血管内皮功能障碍，增加NO的含量（均P<0.01）。另外，与正常对照组相比，DM大鼠血管组织中MDA的含量增加(P<0.01)，抗氧化酶SOD和CAT的活性下降(P<0.01)；ALA治疗可显著降低DM大鼠血管组织中MDA的含量(P<0.05)，增加抗氧化酶SOD和CAT的活性(P<0.01)。结论： ALA可显著改善T2DM模型大鼠的血管内皮舒张功能障碍，其机制可能与减轻血管组织的氧化应激有关。
Abstract:
AIM:To investigate the effects of alpha-linolenic acid (ALA) on vascular function and oxidative stress in diabetic rats. METHODS: An experimental type 2 diabetes mellitus rat model was induced by feeding male Sprague Dawley (SD) rats a high-fat diet for 4 weeks and injecting streptozocin (STZ) i.p. at 30 mg/kg into the rats. Thirty SD rats were randomized equally into three groups: control group, diabetic group and ALA group. The rats were killed and the descending aorta was isolated 4 weeks later. Vascular function, nitric oxide (NO) production, malondialdehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) were determined. RESULTS: Significant endothelial dysfunction and decreased NO production were observed in diabetic rats compared with the control group. Treatment with ALA significantly preserved endothelium-dependent vasorelaxation and increased NO production (both P<0.01 vs. diabetic group). Furthermore, treatment with ALA reduced MDA production and promoted SOD and CAT activities in the aorta compared with those in the diabetic group. CONCLUSION: These results indicate that an ALA-rich diet preserves endothelium-dependent vasorelaxation in diabetic rats, which may be related to the anti-oxidative effect of ALA.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2010-10-20.基金项目：国家自然科学基金项目资助（81070184） 通讯作者：张利华，主任医师，主要从事心血管疾病的研究Email：zlh1204@sohu.com 作者简介：张薇，主治医师，博士Email：yayazw1007@163.com
更新日期/Last Update: 2011-11-03