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SDF-1α在大鼠静脉移植物再狭窄中的作用(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2012年第2期
页码:
177-180
栏目:
基础研究
出版日期:
2012-04-25

文章信息/Info

Title:
Effects of SDF-1α on vein graft restenosis in rats
作者:
刘 超; 李 飞; 段慧娟; 蔺 杰; 张荣庆; 郭文怡
(第四军医大学西京医院心血管内科，陕西 西安 710032)
Author(s):
LIU Chao;  LI Fei;  DUAN Hui-juan;  LIN Jie;  ZHANG Rong-qing;  GUO Wen-yi
(Department of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi, China)
关键词:
基质衍生因子-1α;  血管内膜; 静脉移植物; 大鼠
Keywords:
stromal cell-derived factor-1α;  tunica intima;  vein graft;  rat
分类号:
R392.12
DOI:
-
文献标识码:
A
摘要:
目的:观察基质细胞衍生因子（SDF）-1α在大鼠自体颈外静脉移植物新生内膜增厚中的作用。方法：将60只体质量在250~300 g的 SD雄性大鼠随机分为模型组和CXC趋化因子受体4拮抗剂（AMD3100）组［AMD3100 1mg/(kg·d)×7腹腔注射］，每组30只，建立大鼠自体颈外静脉端侧吻合到颈总动脉的移植物模型后，给予不同的干预。两组大鼠分别在术后0、3、7、14及28 d取静脉移植物，经HE染色后观察移植物新生内膜的厚度；用免疫荧光染色法检测SDF-1α的表达。结果：随着时间的延长，模型组移植物新生内膜的厚度逐渐增加（P<0.01）。与模型组相同时间点相比较，AMD3100组新生内膜的厚度减少（P<0.01）；免疫荧光染色法显示，SDF-1α在移植物中的表达增加，术后7 d表达达到高峰，术后28 d仍能检测到SDF-1α表达。结论：SDF-1α参与了静脉移植物新生内膜的增厚，AMD3100能减轻新生内膜的增厚。
Abstract:
AIM:To evaluate the effects of SDF-1α on neointimal hyperplasia of vein graft in rats. METHODS: Autologous vessels of external jugular were end-to-side grafted into carotid arteries of rats. Sixty Sprague Dawley rats weighing 250-300 g were randomly divided into two groups: model group and AMD3100 group. The vein grafts were harvested at 0, 3, 7, 14 and 28 days postoperatively and vessel wall thickness was measured. Immunofluorescence was used to detect the expression of SDF-1α on vein grafts. RESULTS: Results demonstrated that neointimal thickness of vein graft progressed gradually (P<0.01). The growth of vessel walls in the AMD3100 group decreased compared with that in the model group and protein expression of SDF-1α increased in vein grafts. Staining of SDF-1α emerged at 3 days after surgery and extended to 14 days. Protein expression of SDF-1α could still be detected at 28 days after grafting. CONCLUSION: SDF-1α plays an important role in the growth of vessel wall, and AMD3100 can reduce the thickness of vein graft.

参考文献/References

[1]Li F,Guo W Y,Li W J,et al.Cyclic stretch upregulates SDF-1 alpha/CXCR4 axis in human saphenous vein smooth muscle cells[J].Biochem Biophys Res Commun,2009,386(1):247-251.

[2]Schober A,Knarren S,Lietz M,et al.Crucial role of stromal cell-derived factor-1alpha in neointima formation after vascular injury in apolipoprotein E-deficient mice[J].Circulation,2003,108(20):2491-2497.

[3]Young KC,Torres E,Hatzistergos KE,et al.Inhibition of the SDF-1/CXCR4 axis attenuates neonatal hypoxia-induced pulmonary hypertension[J].Circ Res,2009,104(11):1293-1301.

[4]Zhang LN,Wilson DW,Da CV,et al.Endothelial NO synthase deficiency promotes smooth muscle progenitor cells in association with upregulation of stromal cell-derived factor-1alpha in a mouse model of carotid artery ligation[J].Arterioscler Thromb Vasc Biol,2006,26(4):765-772.

[5]Takahashi M.Role of the SDF-1/CXCR4 system in myocardial infarction[J].Circ J,2010,74(3):418-423.

[6]Zernecke A,Schober A,Bot I,et al.SDF-1alpha/CXCR4 axis is instrumental in neointimal hyperplasia and recruitment of smooth muscle progenitor cells[J].Circ Res, 2005,96(7):784-791.

[7]Muto A,Model L,Ziegler K,et al.Mechanisms of vein graft adaptation to the arterial circulation: insights into the neointimal algorithm and management strategies[J].Circ J,2010,74(8):1501-1512.

[8]Stark VK,Hoch JR,Warner TF,et al.Monocyte chemotactic protein-1 expression is associated with the development of vein graft intimal hyperplasia[J].Arterioscler Thromb Vasc Biol,1997,17(8):1614-1621.

[9]Mayr M,Zampetaki A,Sidibe A,et al.Proteomic and metabolomic analysis of smooth muscle cells derived from the arterial media and adventitial progenitors of apolipoprotein E-deficient mice[J].Circ Res,2008,102(9):1046-1056.

[10]Hu Y,Mayr M,Metzler B,et al.Both donor and recipient origins of smooth muscle cells in vein graft atherosclerotic lesions[J].Circ Res,2002,91(7):e13-e20.

[11]Abi-Younes S,Sauty A,Mach F,et al.The stromal cell-derived factor-1 chemokine is a potent platelet agonist highly expressed in atherosclerotic plaques[J].Circ Res,2000,86(2):131-138.

备注/Memo

备注/Memo:
收稿日期：2011-09-17.基金项目：陕西省科学技术研究发展计划项目资助（2010k16-04-03） 通讯作者：郭文怡，主任医师，主要从事冠心病基础和临床研究 Email：wenyiguo@yahoo.com 作者简介：刘超，硕士生 Email：liulongchao-2009@163.com
更新日期/Last Update: 2012-04-01