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人血白蛋白包裹猪肺动脉脱细胞纤维组织方法的建立(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2012年第2期
页码:
181-184
栏目:
基础研究
出版日期:
2012-04-25

文章信息/Info

Title:
Studies on approaches of human albumin covering decellularized fiber tissue
作者:
郭海平1; 李温斌2; 郭俊平1; 靳振生1; 薛丽霞1; 崔鹤仙1; 梁建国1
(1.邯郸市第一医院心胸外科，河北 邯郸 056002；2.首都医科大学附属北京安贞医院心脏外科，北京 100029)
Author(s):
GUO Hai-ping1;  LI Wen-bin2;  GUO Jun-ping1;  JIN Zhen-sheng1;  XUE Li-xia1;  CUI He-xian1;  LIANG Jian-guo1
(1.Department of Cardio-thoracic Surgery, Handan First Hospital, Handan 056002, Hebei, China; 2.Department of Cardiac Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China)
关键词:
白蛋白; 组织工程; 心脏瓣膜; 肺动脉; 猪
Keywords:
albumin;  tissue engineering;  heart valve;  pulmonary;  porcine
分类号:
Q512.1
DOI:
-
文献标识码:
A
摘要:
目的:建立以人血白蛋白包裹脱细胞猪肺动脉纤维支架的方法。方法：采用自主研制的“脱水浸泡法”将人血白蛋白渗透至脱细胞猪肺动脉纤维支架内，比较脱细胞纤维组织和经白蛋白包裹的脱细胞纤维组织在物理性能方面的异同。通过免疫组织化学染色法评价白蛋白包裹脱细胞组织的效果。结果：采用“脱水浸泡法”能够使白蛋白有效地结合在脱细胞纤维组织内，完成人血白蛋白对脱细胞纤维组织的全面包裹。结论：采用“脱水浸泡法”，人血白蛋白能够充分包裹脱细胞纤维支架，同时人血白蛋白包裹的脱细胞纤维组织的物理性能优于单纯脱细胞纤维组织。
Abstract:
AIM:To observe the approach of human albumin covering decellularized fiber tissue. METHODS: Human albumin was penetrated into decellularized porcine pulmonary fiber scaffolds by the method of “dewatering and soaking.” We next examined the similarities and differences of physical features between decellularized tissue and human albumin-decellularized tissue. The effects of human albumin covering decellularized tissue were evaluated by immunohistochemical methods. RESULTS: Using the “dewatering and soaking” method, human albumin could effectively combine with decellularized porcine pulmonary fiber scaffolds. Human albumin completely covered decellularized tissue. CONCLUSION: Using the “dewatering and soaking” method, human albumin may effectively combine with decellularized porcine pulmonary fiber scaffolds. Human albumin-decellularized tissue was superior in physical features to decellularized tissue.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2011-04-12.通讯作者： 李温斌，主任医师，主要从事心脏外科及心脏组织 工程瓣膜的研究 Email:Liwenbin65@vip.sina.com 作者简介：郭海平，主治医师，硕士 Email:15833306918@163.com
更新日期/Last Update: 2012-04-01