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《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:

2012年第3期

页码:

335-339

栏目:

临床研究

出版日期:

2012-06-25

文章信息/Info

Title:

Expression of Notch1 signaling and inflammatory cytokines in peripheral blood mononuclear cells of patients with acute coronary syndrome

作者:

付文波¹; 2; 丁世芳² ; 李大主¹; 何少林¹; 王 妍¹; 黎 明¹; 冯义柏¹

(1.华中科技大学同济医学院协和医院心内科、心血管病研究所，湖北 武汉 430022；2.广州军区武汉总医院心内科，湖北 武汉 430070)

Author(s):

FU Wen-bo¹; 2;  DING Shi-fang²;  LI Da-zhu¹;  HE Shao-lin¹;  WANG Yan¹;  LI Ming¹;  FENG Yi-bai¹

(1.Institute of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei, China; 2.Department of Cardiology, Wuhan PLA General Hospital, Wuhan 430070, Hubei, China)

关键词:

急性冠脉综合征; 巨噬细胞; Notch1信号通路; 动脉粥样硬化

Keywords:

acute coronary syndrome;  platelet-monocyte aggregates;  Notch;  atherosclerosis

分类号:

R543.3

DOI:

-

文献标识码:

A

摘要:

目的:探讨在急性冠脉综合征（acute coronary syndrome，ACS）患者外周血单核-巨噬细胞中Notch1分子及炎性细胞因子的表达。方法： 以密度梯度离心法分离36例ACS患者、14例稳定型心绞痛患者及30例健康对照者外周血单核细胞，并以氟波酯（PMA）使之转化为巨噬细胞。分别采用实时（RT）-PCR和Western blot测定巨噬细胞中Notch1 mRNA和其蛋白的表达；采用RT-PCR和ELISA测定炎性细胞因子血管细胞黏附分子-1(VCAM-1) mRNA和其蛋白及单核趋化蛋白-1(MCP-1) mRNA和其蛋白的表达。结果： 与对照组比较，ACS患者巨噬细胞中Notchl mRNA和其蛋白的表达明显升高(P<0.05)，ACS诱导的VCAM-1和MCP-1 mRNA和二者的蛋白浓度明显增高(P<0.05)。结论： Notch1信号通路及其介导的巨噬细胞天然免疫应答在动脉粥样硬化易损斑块的发生发展中发挥着重要的调节作用。

Abstract:

AIM:To explore the differential expression of Notch signaling in human platelet-monocyte aggregates (PMAs) of patients with acute coronary syndrome (ACS). METHODS: PMAs of 50 patients with coronary artery disease (CAD) including 36 patients with ACS and 14 patients with stable angina (SA) were measured. Thirty subjects without CAD served as controls. After 48 h, cells and supernatant were collected separately. Expressions of Notch1 mRNA were measured by real-time quantitative PCR (RT-qPCR) and protein was measured by Western blot. Levels of vascular cell adhesive molecule-1 (VCAM-1) and monocyte chemoattractant protein-1 (MCP-1) in supernatant were determined by ELISA. RESULTS: Notch1 mRNA and protein expressions in ACS group were significantly higher than in controls (P<0.05). Levels of VCAM-1 and MCP-1 significantly increased in the CAD group (P<0.05). CONCLUSION: Notch1 and Notch1-mediated innate immune response may play an important regulatory role in the development and progression of atherosclerosis.

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备注/Memo

备注/Memo:

收稿日期：2012-02-18.基金项目：国家自然科学基金项目资助（81172781） 通讯作者：冯义柏，主任医师，主要从事心血管病临床与基础研究Email:yibaifeng688@hotmail.com 共同通讯作者：黎明，副主任医师，主要从事心血管病临床与基础研究 Email:liming518888cn@yahoo.com.cn 作者简介：付文波，副主任医师，博士生 Email: fuwenbo10@sina.com

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更新日期/Last Update: 2012-05-02