粤北地区早发冠心病与MEF2A基因11外显子的关系
《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]
- 期数:
- 2012年第6期
- 页码:
- 715-718
- 栏目:
- 临床研究
- 出版日期:
- 2012-12-25
文章信息/Info
- Title:
- Relationship between exon 11 of MEF2A gene and premature coronary artery disease in Yuebei area
- Author(s):
- ZHANG Bei-bei; XU Xin; MA Shao-chun; TANG Liang-qiu; ZHANG She-bing; HE Feng-ping; CHEN Bao-feng
- (Department of Cardiovasology, Affiliated Yuebei People’s Hospital, Medical College, Shantou University, Shaoguan 512026, Guangdong, China)
- Keywords:
- coronary artery disease; MEF2A; polymorphism
- 分类号:
- R541.4
- DOI:
- -
- 文献标识码:
- A
- 摘要:
- 目的:探讨广东粤北地区人群中肌细胞增强因子（MEF）2A基因第11外显子基因多态性与早发冠心病的关联性。方法： 应用聚合酶链反应-单链构象多态性和DNA测序技术检测210例散发冠心病患者及180例对照者MEF2A基因第11外显子基因多态性，并研究它与冠心病的关系。结果： 冠心病常见的危险因素，如高血压病、糖尿病、吸烟，在冠心病组分布的频率明显高于对照组（P<0.05）,MEF2A基因11号外显子存在CAG重复序列多态性，重复序列4～11个不等，各等位基因的分布在冠心病组和对照组无明显差异，未发现21个碱基缺失的基因突变。采用Logistic回归方程分析，只有糖尿病是冠心病发病的独立危险因素（P<0.01）。结论： MEF2A基因第11外显子CAG重复序列基因多态性不是粤北地区早发冠心病的危险因素。
- Abstract:
- AIM:To investigate the association between the polymorphism in exon 11 of MEF2A and premature coronary artery disease (CAD) in the Yuebei area of Guangdong. METHODS: The variation analysis of exon 11 of MEF2A gene was performed using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and DNA direct sequencing in 210 sporadic patients with CAD and 180 controls without CAD and its correlation with CAD was analyzed. RESULTS: Frequencies of the classical risk factors for CAD such as hypertension, diabetes and smoking were higher in patients than in controls (P<0.05). CAG repeat polymorphism was detected in exon 11 of MEF2A gene and the number of CAG repeats ranged from 4 to 11, with no significant difference in the distribution of CAG alleles between patients with CAD and controls. No 21-bp deletion was detected in any subject of the study population. Logistic regression analysis revealed that only diabetes was an independent risk factor for CAD (P<0.01). CONCLUSION: CAG repeat polymorphism in exon 11 of MEF2A gene is not a risk factor for premature CAD in Yuebei area.
参考文献/References
[1]Wang L,Fan C,Topol SE,et al.Mutation of MEF2A in an inherited disorder with features of corornary artery disease[J].Science,2003,302(5650):1578-1581.[2]Olson EN.Coronary artery disease and the MEF2A transcription factor[J].Sci Aging Knowledge Environ,2003,2003(48):pe33.[3]Elhawari S,Al-Boudari O,Muiya P,et al.A study of the role of the Myocyte-specific Enhancer Factor-2A gene in coronary artery disease[J].Atherosclerosis,2010,209(1):152-415.[4]Maiolino G,Colonna S,Zanchetta M,et al.Exon 11 deletion in the myocyte enhancer factor (MEF)2A and early onset coronary artery disease gene in a Sicilian family[J].Eur J Cardiovasc Prev Rehabil,2011,18(4):557-560.[5]Guella I,Rimoldi V,Asselta R,et al.Association and functional analyses of MEF2A as a susceptibility gene for premature myocardial infarction and coronary artery disease[J].Circ Cardiovasc Genet,2009,2(2):165-172.[6]Lieb W,Mayer B,Knig IR,et al.Lack of association between the MEF2A gene and myocardial infarction[J].Circulation, 2008,117(2):185-191.[7]Hsu LA,Chang CJ,Teng MS,et al.CAG repeat polymorphism of the MEF2A gene is not associated with the risk of coronary artery disease among Taiwanese[J].Clin Appl Thromb Hemost,2010,16(3):301-305.[8]Dai DP,Zhou XY,Xiao Y,et al.Structural changes in exon 11 of MEF2A are not related to sporadic coronary artery disease in Han Chinese population[J].Eur J Clin Invest,2010,40(8):669-677.[9]Han Y,Yang Y,Zhang X,et al.Relationship of the CAG repeat polymorphism of the MEF2A gene and coronary artery disease in a Chinese population[J].Clin Chem Lab Med,2007,45(8):987-992.[10]Liu Y,Niu W,Wu Z,et al.Variants in exon 11 of MEF2A gene and coronary artery disease:evidence from a case-control study,systematic review, and meta-analysis[J]. PLoS One,2012,7(2):e31406.
备注/Memo
- 备注/Memo:
- 收稿日期：2012-08-24.作者简介：章蓓蓓，住院医师，硕士 Email:26744074@qq.com
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