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Title:: Study about the changes of transmural dispersity repolarization and connexin 43 in heart failure rabbit

作者:: 芦兰¹; 师幸伟²; 程冕³; 白融⁴; 阮磊³; 谢刚²; 宋玉娥⁴; (长江航运总医院：1.肿瘤科，2.心内科，湖北武汉 430000；华中科技大学同济医学院附属同济医院：3.综合科，4.心内科，湖北武汉 430020)

Author(s):: LU Lan¹; SHI Xingwei²; CHENG Mian³; BAI Rong⁴; RUAN Lei³; XIE Gang²; SONG Yu e⁴; (1.Department of oncology, 2.Department of Cardiology, General Hospital, Yangtze River Shipping, Wuhan 430000, Hubei, China; 3.General Department, 4.Department of Cardiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China)

关键词:: 心力衰竭; 单相动作电位; 跨室壁复极离散度; 缝隙连接蛋白43; 兔

Keywords:: heart failure; rabbit; monophasic action potential; Transmural dispersion of repolarization; connexin 43

分类号:: R541.7

DOI:: -

文献标识码:: A

摘要:: 目的:探讨阿霉素诱导的兔心力衰竭(heart failure，HF)时跨室壁离散度和缝隙连接蛋白43的变化。方法:将18只日本大耳白兔随机分为对照组(n=10)及模型组(n=8)。采用阿霉素诱导建立兔HF模型。采用单相动作电位（monophasic action potential，MAP）记录技术，分别记录HF组和对照组左室心肌内、中、外膜的MAP，分别测量获得20列三层心室肌的90%动作电位时程（monophasic action potential duration，MAPD90），计算并分析跨室壁复极离散度（transmural dispersity of repolarization，TDR）的变化，TDR为每列中MAPD90 max与MAPD90 min的差值。应用免疫荧光染色法检测缝隙连接蛋白43(gap junction connexin43，CX43)在心肌细胞间分布的变化。结果:与对照组比较HF组的MAPD90缩短，TDR则明显增大（P<0.05）。免疫荧光染色法检测显示，HF组CX43分布与对照组有所差别。结论:HF组心脏TDR增加，CX43分布发生改变，其可能与HF时恶性心律失常的发生有关。

Abstract:: AIM:To investigate the the changes of transmural dispersity repolarization and connexin 43 in heart failure rabbit. METHODS: Models of adriamycininduced heart failure heart were established in rabbits and picardial, mid and endometrial monophasic action potentials (MAP) in the left ventricular myocardium were recorded with MAP recording technology, monophasic action potential duration MAPD90 was measured, changes of transmural dispersion of repolarizzation (TDR) was analyzed, and the distribution changes of gap junction Connexin43 (CX43) in the myocardial cells were observed by immunofluorescence. RESULTS: Compared with those in control group, MAPD90 in heart failure group was shortened and TDR significantly increased (P<005). Immunofluorescence showed differences in CX43 distribution between heart failure group and control group. CONCLUSION: The increased TDR and the distribution changes of CX43 in heart failure may be related to occurrence of malignant ventricular arrhythmia.

参考文献/References

[1]Hunt SA,Abraham WT,Chin MH,et al.2009 Focused Update incorporated into the ACC/AHA Guidelines for the Diagnosis and Management of Heart Failure in Adults:a Report of the American College of Cardiology Foundation/American Heart Association Task Force on Practice Guidelines. Developed in collaboration with the International society for Heart and Lung Transplantation[J].J Am Coll Cardiol, 2009,53(15):e1-e90.
[2]师幸伟,程冕，白融，等.阿霉素诱导兔心衰模型的建立与评价[J].动物医学进展,2013,34(3):44-47.
[3]李泱,马杰,肖建民,等.应用自制复合电极同步记录兔左心室游离壁三层心肌的单相动作电位[J].中国心脏起搏与心电生理杂志,2003,17(2):142-145.
[4]刘元生.心肌M细胞[J].临床心电学杂志,2010,19(3):236.
[5]Burton FL,Cobble SM.Dispersion of ventricular repolarization and refractory period[J].Cardiovasc Res,2001,50(1):10-23.
[6]Poelzing S,Rosenbaum DS.Altered connexin43 expression produces arrhythmia substrate in heart failure[J].Am J Physiol Heart Circ Physiol,2004,287(4):H1762-H1770.
[7]Strom M,Wan X,Poelzing S,et al.Gap junction heterogeneity as mechanism for electrophysiologically distinct properties across the ventricular wall[J].Am J Physiol Heart Circ Physiol,2010,298(3):H787-H94.

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兔心力衰竭时跨室壁复极离散度和缝隙连接蛋白43变化的研究

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

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