我们的网站为什么显示成这样？

可能因为您的浏览器不支持样式，您可以更新您的浏览器到最新版本，以获取对此功能的支持，访问下面的网站，获取关于浏览器的信息：

|本期目录/Table of Contents|

肥胖对家兔心房肌组织结构和电生理特性的影响

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2014年第1期
页码:
24-28
栏目:
基础研究
出版日期:
2013-12-02

文章信息/Info

Title:
IInfluence of obesity on rabbit atrial muscle tissue structure and electrical physiological characteristics
作者:
曾广伟; 刘雄涛; 郑强荪; 尚福军; 于 亮; 曾 迪
(第四军医大学唐都医院心内科，陕西 西安 710038)
Author(s):
ZENG Guang-wei;  LIU Xiong-tao;  ZHENG Qiang-sun;  SHANG Fu-jun;  YU Liang;  ZENG Di
(Department of Cardiology, Tangdu Hospital, Fourth Military Medical University, Xi’an 710038, Shaanxi, China)
关键词:
肥胖; 心房肌; 电生理特性; 纤维化; 细胞凋亡; 家兔
Keywords:
obesity;  atrial muscle;  electrophysiological characteristics;  fibrosis;  cell apoptosis;  rabbit
分类号:
R151.1；R331.38
DOI:
-
文献标识码:
A
摘要:
目的:探讨肥胖对家兔心房肌组织结构和电生理特性的影响，为进一步了解肥胖影响房颤(AF)易感性的相关机制和AF的早期干预提供实验依据。方法:将30只家兔随机分为正常组、肥胖组和肥胖+他汀药物组，雌雄各半，每组10只。肥胖组给予高脂饲料，造模成功后，采用Langendorff离体灌注法对3组家兔分别进行离体心脏灌注。采用心脏电生理刺激仪监测心房肌的电生理特性参数: 肥胖组家兔房内传导时间（HRA-HIS）、心房有效不应期(AERP)、窦房结恢复时间(SNRT)、校正后窦房结恢复时间(CSNRT)。剪取部分心耳组织， 经MASSON染色观察心房肌纤维化的程度。采用TUNEL法测定心房肌细胞的凋亡率。结果:与正常组相比，肥胖组家兔HRA-HIS、AERP、SNRT和CSNRT等电生理参数均出现不同程度的延长，并导致AERP频率自适应性降低。与肥胖组比较，肥胖+他汀药物组的心肌基质胶原纤维显著减少（P<0.05），心肌胶原容积分数(CVF)显著减低（P<0.01），心肌细胞凋亡率(%)明显降低（P<0.01）。结论:肥胖可引起家兔心房肌组织结构与电生理基质改变，利于AF的发生与维持，而应用他汀类药物干预后，这些改变有所改善。
Abstract:
AIM:To explore the influence of obesity on rabbit atrial muscle tissue structure and electrical physiological characteristics and to better understand the mechanism of atrial fibrillation (AF) susceptibility induced by obesity and to provide an experimental basis for early intervention AF. METHODS: Thirty rabbits were randomly divided into three groups: normal group, obesity group and obesity statin group, with ten rabbits in each group. After the successful establishment of the obesity model, Langendorff perfusion in vitro was used for rabbit heart perfusion in vitro. The cardiac electrophysiological stimulator was then used to detect the electrical physiological characteristic parameters including atrium room conduction time (HRA-HIS), atrial effective refractory period (AERP), sinoatrial node recovery time (SNRT), and corrected sinoatrial node recovery time (CSNRT). After shearing part of the rabbit auricle tissue, atrial muscle fibrosis was observed using Masson stain and atrial muscle cell apoptosis was detect by TUNEL. RESULTS: The electrical physiological parameters including HRA-HIS, AERP, SNRT and CSNRT were all extended to some degree and AERP frequency adaptability significantly reduced in obesity group. The myocardial matrix collagen fiber and CVF obviously decreased, whereas cardiomyocyte apoptosis rate significantly increased in the obesity group compared with those in the obesity statin group (all P<0.05). CONCLUSION: Obesity can change the rabbit atrial muscle tissue structure and electrical physiological characteristics, which is propitious to the occurrence and maintenance of AF.

参考文献/References

[1]逯建华,汤迎春.心房颤动的诊断与治疗进展[J].中国现代医药杂志,2011,13(3):134-135.
[2]翁银燕.肥胖与房颤关系的临床研究[D].哈尔滨:哈尔滨医科大学,2009.
[3]马 君,李学文.房颤发生与维持的研究进展[J].中西医结合心脑血管病杂志,2011,9(4):474-477.
[4]马利芹，耿光瑞，孙秀梅.大黄减肥复方对家兔脂肪代谢的影响[J].黑龙江畜牧兽医,2009(3):107-108.
[5]王国贤，王蕾蕾.葛根素对糖尿病大鼠心肌纤维化的影响[J].江苏大学学报(医学版),2011,21(4):294-297.
[6]陈 凡，颜宇波，杨天伦,等.氯沙坦和曲尼司特抗高血压大鼠心肌纤维化的对比观察[J].中国现代医学杂志,2010,20(7):1000-1007.
[7]聂园园,仇小强.心肌细胞凋亡与心血管疾病关系的研究进展[J].实用医学杂志,2012,28(2):324-326.
[8]Chiou CW,Eble JN,Zipes DP.Efferent vagal innervation of the canine atria and sinus and atrioventricular nodes.The third fat pad[J].Circulation,1997,95(11):2573-2584.
[9]Teresa SM.肥胖系阵发性房颤进展为永久性房颤的危险因素之一[J].国外医学·老年医学分册,2009,30(5):237-238.
[10]Wang TJ,Parise H,Levy D,et al.Obesity and the risk of new-onset atrial fibrillation[J].JAMA,2004,292(20):2471-2477.
[11]Malavazos AE,Cereda E,Morricone L,et al. Monocyte chemoattractant protein 1 (MCP-1) and visceral fat[J].J Clin Endocrinol Metab,2005,90(5):3128.
[12]Kremen J,Dolinkova M,Krajickova J,et al.Increased subcutaneous andepicardial adipose tissue production of proinflammatory cytokines in cardiac surgery patients: possible role in postoperative insulin resistanc[J].J Clin Endocrinol Metab,2006,91(11):4620-4627.
[13]王洪涛,郑强荪,杨玉辉,等.犬心脏第3脂肪垫对左心房及左上肺静脉电生理特性的影响[J].心脏杂志,2006,18(4):386-387.
[14]杨玉辉,郑强荪,董建军,等.消融犬右肺静脉脂肪垫对心房及右上肺静脉电生理特性及房颤诱发的影响[J].心脏杂志,2006,18(1):28-30.
[15]Werner N,Nickenig G,Laufs U. Pleiotropic effects of HMG-CoA reductase inhibitors[J].Basic Res Cardiol,2002,97(3):105-116.
[16]Zhang J,Cheng X,Liao YH,et al.Simvastatin regulates myocardial cytokine expression and improves ventricular remodeling in rats after acute myocardial infarction[J].Cardiovasc Drugs Ther, 2005,19(1):13-21.
[17]Wallace CK,Stetson SJ,Kucuker SA,et al.Simvastatin decreases myocardial tumor necrosis factor alpha content in heart transplant recipients[J].J Heart Lung Transplant,2005,24(1):46-51.
[18]尚福军,王捷频,郑强荪,等.辛伐他汀抑制大鼠心肌细胞TNF-α的表达及其与活性氧的关系[J].中国临床药理学与治疗学,2009,14(12):1379-1385.

备注/Memo

备注/Memo:
收稿日期：2013-04-25.通讯作者：郑强荪，教授，主要从事心血管电生理研究 Email：qingshu@fmmu.edu.cn 作者简介：曾广伟，住院医师，硕士 Email：zengguangwei1026@163.com
更新日期/Last Update: 2014-01-20