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《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:

2014年第2期

页码:

129-132146

栏目:

基础研究

出版日期:

2014-01-20

文章信息/Info

Title:

Effect of bushen huoxue decoction on E2 and vasomotor function

作者:

宋欢欢¹; 曹 炎²; 王玉珍¹; 张玉芬¹; 赵 娇¹; 吴雷涛¹; 马 静¹

(第四军医大学：1.西京医院中医科，2.航空航天医学系，陕西 西安 710032)

Author(s):

SONG Huan-huan¹;  CAO Yan²;  WANG Yu-zhen¹;  ZHANG Yu-fen¹;  ZHAO Jiao¹;  WU Lei-tao¹;  MA Jing¹

(1.Department of Traditional Chinese Medicine, Xijing Hospital, 2.Department of Aerospace Medicine, Fourth Military Medical University, Xi’an 710032, Shaanxi, China)

关键词:

补肾活血汤; 去卵巢大鼠; 雌二醇; 血管舒缩功能

Keywords:

bushen huoxue decoction;  ovariectomized rats;  estradiol;  vasomotor function

分类号:

R977.12

DOI:

-

文献标识码:

A

摘要:

目的:观察补肾活血汤(bushen huoxue decoction，BHD)对去卵巢大鼠血清雌二醇（estradiol，E2）和血管舒缩功能的影响。方法： 56只成年雌性SD大鼠随机分为7组：空白组、假手术组、模型组、去卵巢+BHD高、中、低剂量组及去卵巢+替勃龙组(替勃龙组)，每组8只。采用免疫法测定实验大鼠血清E2、促黄体激素(luteinizing hormone，LH)和促卵泡激素(follicle-stimulating hormone，FSH)的含量。观察补肾活血汤对给予去甲肾上腺素（noradrenaline，NE）和乙酰胆碱（acetylcholine，Ach）后的离体大鼠腹主动脉血管环产生舒缩的活性的影响，经HE染色后，用光镜观察腹主动脉结构的变化。结果： 与模型组相比，BHD各剂量组可显著升高模型大鼠血清E2的水平(P<0.05），显著降低LH和FSH的水平(P<0.05)，使模型大鼠腹主动脉对缩血管物质的反应降低，加NE后模型组血管张力增加显著（P<0.05），血管顺应性较好，舒缩功能得到改善，BHD高剂量组优于BHD中、低剂量组。血管中平滑肌细胞较模型组清晰，组织结构排列整齐、紧密，与替勃龙组无显著差异。结论： BHD可使腹主动脉对缩血管物质NE的反应减轻，血管顺应性增强，血管舒缩功能得到改善，血管结构的破坏程度减轻，其机制可能与增加的E2含量有关。

Abstract:

AIM:To observe the effect of bushen huoxue decoction (BHD) on serum E2 and vasomotor function of ovariectomized rats. METHODS: Fifty-six adult female Sprague Dawley rats were randomly divided into seven groups including eight rats each: control group, sham operated group, model group, ovariectomy plus BHD in high-, medium-, low-dose groups, and ovariectomy plus tibolone group (tibolone group). Estradiol, luteinizing hormone and follicle-stimulating hormone in serum of the rats were measured by using immunoassay. After delivery of noradrenaline (NE) and acetylcholine (Ach), the effect of BHD on vasomotor function of rat abdominal aorta was observed in vitro. Hematoxylin/eosin staining was used to observe the structural changes of abdominal aorta. RESULTS: Compared with those in the model group, levels of serum E2 in BHD groups remarkably increased (P<0.05) and FSH and LH levels were observably reduced (P<0.05). Vasoconstrictor responses to abdominal aorta were reduced in the model group and, after NE intervention, the vascular tension in the model group significantly increased (P<0.05). Vascular compliance and diastolic function in BHD groups were improved, especially in the high-dose group. Compared with those in the model group, vascular smooth muscle cells were in clearer and with better structure and neatly and closely arranged in BHD groups. No significant difference was found compared with those in tibolone group. CONCLUSION: BHD relieves the abdominal aorta vasoconstrictor response to NE, improves vasomotor functions and reduces the extent of damage to the vascular structure. Its mechanism may be associated with increased E2 content.

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备注/Memo

备注/Memo:

收稿日期：2013-08-20.
基金项目：陕西省科学技术研究发展计划项目资助（2012K19-05-07）
通讯作者：马静，副教授，主要从事中西医结合防治心血管病及机制研究Email：jingma@fmmu.edu.cn
作者简介：宋欢欢，硕士生Email：32095222@qq.com

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更新日期/Last Update: 2014-01-16