丙酮酸对缺血/再灌注大鼠心肌的保护作用
《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]
- 期数:
- 2014年第2期
- 页码:
- 143-146
- 栏目:
- 基础研究
- 出版日期:
- 2014-01-20
文章信息/Info
- Title:
- Protection of pyruvate on heart against ischemia/reperfusion injury
- Author(s):
- XU Jie; ZHANG Xing; QIN Xing-hua; YANG Lu; XING Yuan; GAO Feng
- (Department of Physiology, School of Basic Medical Sciences, Fourth Military Medical University, Xi’an 710032, Shaanxi, China)
- Keywords:
- pyruvate; ischemia/reperfusion; cardiac function; rat
- 分类号:
- Q555.7
- DOI:
- -
- 文献标识码:
- A
- 摘要:
- 目的:探讨丙酮酸(Pyr)对缺血/再灌注（I/R）大鼠心肌的影响及其可能的机制。方法： 30只SD成年大鼠随机分为假手术(Sham)组、I/R组及I/R+Pyr组，每组10只。I/R+Pyr组大鼠于再灌前5 min，开始持续性静脉灌注Pyr 2 h［25 mg/(kg·h)］。再灌注2 h后，利用多道生理记录仪检测大鼠在体血流动力学指标：平均动脉压（MABP）、左室收缩压（LVSP）及左室最大收缩、舒张末压微分（±LV dP/dtmax）。采用Western blot方法检测磷酸化-JNK(p-JNK)和总的JNK(t-JNK)表达。用原位缺口末端标记法(TUNEL)评价心肌细胞的凋亡。结果： I/R组的MABP、LVSP、±LV dP/dtmax显著低于Sham组（P<0.01），Pyr干预可增加I/R后MABP、LVSP及±LV dP/dtmax的水平（P<0.05）。与Sham组相比，I/R组大鼠左心室p-JNK的水平明显增高（P<0.01）；而Pyr可降低大鼠左心室p-JNK的水平（P<0.01），并抑制心肌细胞凋亡（P<0.05）。结论： Pyr可改善I/R大鼠心肌的功能，抑制心肌细胞凋亡，其机制可能与抑制JNK信号的激活有关。
- Abstract:
- AIM:To investigate the effect of pyruvate on myocardial ischemia/reperfusion (I/R) injury and the underlying mechanism. METHODS: The animal model of myocardial I/R injury was induced by 30 min of left anterior descending coronary occlusion followed by 2 h of reperfusion. Thirty rats were randomly divided into three groups: sham group, I/R group and I/R plus pyruvate treatment (I/R+Pyr) group. Pyruvate was intravenously infused at 25 mg/(kg·h) for 2 h, 5 min before reperfusion. Hemodynamic parameters (including LVSP, MABP and ±LV dP/dtmax) phosphorylation and total levels of JNK were determined and cardiomyocyte apoptosis was evaluated by TUNEL staining. RESULTS: LVSP, MABP and ±LV dP/dtmax decreased in I/R group and pyruvate treatment reversed these effects. p-JNK level increased in I/R group compared with that in sham group. Pyruvate inhibited p-JNK activation but did not change JNK expression. The index of apoptotic cardiomyocytes decreased with pyruvate treatment compared with that in I/R group. CONCLUSION: Pyruvate improves the myocardial function and inhibits myocardial apoptosis, which may result from the inhibition of JNK phosphorylation in the rat myocardial I/R model.
参考文献/References
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备注/Memo
- 备注/Memo:
- 收稿日期：2013-11-11.
通讯作者：高峰，教授，主要从事胰岛素抵抗与心血管疾病研究Email：fgao@fmmu.edu.cn
作者简介：徐杰，硕士生Email： xujiefmmu@126.com
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