我们的网站为什么显示成这样？

可能因为您的浏览器不支持样式，您可以更新您的浏览器到最新版本，以获取对此功能的支持，访问下面的网站，获取关于浏览器的信息：

|本期目录/Table of Contents|

黄连素对缺氧/复氧诱导的大鼠心肌细胞损伤的拮抗作用

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2014年第3期
页码:
271-273，284
栏目:
基础研究
出版日期:
2014-03-20

文章信息/Info

Title:
Protective effect of berberine on hypoxia reoxygenation-induced apoptosis in myocardial cells
作者:
车奕宏; 王海昌
(第四军医大学西京医院心血管内科，陕西 西安 710032)
Author(s):
CHE Yi hong;  WANG Hai chang
(Department of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi, China)
关键词:
黄连素; 缺氧/复氧损伤; 心肌细胞H9c2; 细胞凋亡
Keywords:
berberine;  hypoxia reoxygenation;  H9C2 cardiac muscle cells;  apoptosis
分类号:
R284.1
DOI:
-
文献标识码:
A
摘要:
目的:探讨黄连素(BR)对大鼠H9c2心肌细胞缺氧/复氧(H/R)损伤后凋亡的影响及其作用机制。方法:将培养的H9c2心肌细胞随机分为：正常对照(NC)组、单纯BR组、H/R组、药物低剂量BR(1.5×10-6 mol/L)组及高剂量BR(1.5×10-4 mol/L)组。处理结束后，用MTT比色法检测H9c2心肌细胞的活力，用Hoechst33258染色检测细胞核形态的变化，用Western blot法检测Cytochrome-C(Cyt-C),Caspase-9蛋白的表达。结果:与NC组比较，单纯BR组对H9c2心肌细胞无影响。与H/R组比较，低、高剂量BR组细胞的活力分别为(60.8±1.9)%和(81.2±1.9)%明显上升(P<0.01)，心肌细胞的凋亡率明显减少，Western blot的结果显示，BR可抑制凋亡相关蛋白Cytochrome-C、Caspase-9的表达。结论:BR对H9c2心肌细胞H/R损伤后的凋亡具有显著的抑制作用，其可能的机制与抑制的Cyt-C的释放和抑制Caspase-9的表达有关。
Abstract:
AIM:To examine the effect of berberine on hypoxia reoxygenationinduced apoptosis in myocardial cells and to explore the potential mechanisms involved. METHODS: H9C2 cardiac muscle cells were divided into: normal control group (NC group), simple berberine group (BR group), hypoxia/reoxygenation group (H/R group) and drug treatment group (divided into LBR group: 1.5×10-6 mol/L, HBR group 1.5×10-4 mol/L). Myocardial cell vitality was detected by MTT, nucleus morphological changes were examined using Hoechst 33258 dye and cytochrome-C and caspase-9 protein expressions were detected by Western blot. RESULTS: Compared with that in the NC group, simple berberine exerted no effect on myocardial cells in the BR group. Compared with those in the H/R group, cell vitality in treatment groups increased and apoptosis decreased significantly. Western blot results showed that berberine inhibited the expressions of apoptosis related proteins cytochrome-C and caspase-9. CONCLUSION: Berberine has a significant protective effect on hypoxia reoxygenation induced apoptosis in myocardial cells possibly by inhibiting the release of cytochrome-C and decreasing the expression of caspase-9.

参考文献/References

[1]Saks VA,Kuznetsov AV,Vendelin M,et al.Functional coupling as a basic mechanism of feed back regulation of cardiac energy metabolism[J].Mol Cell Biochem,2004,256-257(1-2):185-199.
[2]Wang YX,Zheng YM,Zhou XB.Inhibitory effects of berberine on ATP-sensitive K+ channels in cardiac myocytes[J].Eur J Pharmacol,1996,316(2-3):307-315.
[3]Anis KV,Rajeshkumar NV,Kuttan R.Inhibition of chemical carcinogenesis by berberine in rats and mice[J].J Pharm Pharmacol,2001,53(5):763-768.
[4]Amin AH,Subbaiah TV,Abbasi KM.Berberine sulfate:antimicrobial activity,bioassay,and mode of action[J].Can J Microbiol,1969,15(9):1067-1076.
[5]Kong W,Wei J,Abidi P,et al.Berberine is a novel cholesterol-lowering drug working through a unique mechanism distinct from statins[J].Nat Med,2004,10(12):1344-1351.
[6]Glancy B,Balaban RS.Role of mitochond rial Ca2+ in the regulat ion of cellular energetics[J].Biochemistry,51(14):2959-2973.
[7]Wan B,Doumen C,Duszynski J,et al.Effects of cardiac work on electrical potential grad ient across mitochondrial membrane in perfused rat hearts[J]. Am J Physiol,1993,265(2 Pt 2):H453-H460.

备注/Memo

备注/Memo:
收稿日期：2013-09-01.
通讯作者：王海昌，主任医师，主要从事冠心病研究 Email:wanghc@fmmu.edu.cn
作者简介：车奕宏，硕士生 Email:cheyihong01987@163.com
更新日期/Last Update: 2014-03-21