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|本期目录/Table of Contents|

脂联素通过AMPK调节HepG2细胞中支链氨基酸的代谢

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2014年第4期
页码:
416-419
栏目:
基础研究
出版日期:
2014-04-25

文章信息/Info

Title:
Adiponectin improves branched-chain amino acid catabolism of HepG2 cells via upregulation of AMPK
作者:
杜朝升; 朱 迪; 邹 倩; 廉 坤; 洪志博; 周 芬; 陶 凌
(第四军医大学西京医院心血管内科，陕西 西安 710032)
Author(s):
DU Chao-sheng;  ZHU Di;  ZOU Qian;  LIAN Kun;  HONG Zhi-bo;  ZHOU Fen;  TAO Ling
(Department of Cardiology, Xijing Hospital, Fourth Military University, Xi’an 710032, Shaanxi, China)
关键词:
脂联素; AMPK; HepG2细胞系; 支链氨基酸; 代谢
Keywords:
adiponectin;  AMPK;  hepatic tumor cell;  branched- chain amino acids;  catabolism
分类号:
R589.2
DOI:
-
文献标识码:
A
摘要:
目的:探讨脂联素（APN）对HepG2细胞支链氨基酸（BCAA）分解代谢的影响及其作用机制。方法： 将培养的HepG2细胞系随机分为对照组、APN组、APN+腺苷酸活化蛋白激酶(AMPK)抑制剂(Compound C，CpC)组(APN+CpC)组和AMPK激动剂（AICAR）组。用Western blot检测AMPK、P-AMPK、BCKD E1α（BCAA分解代谢过程中的关键酶，磷酸化时为失活状态）及P-BCKD E1α蛋白的表达，用ELISA方法检测培养HepG2细胞上清中BCAA的含量。结果： 与对照组相比，APN组和AICAR组均可以显著上调细胞P-AMPK的水平（P<0.05，P<0.01），显著降低细胞p-BCKD E1α及上清中BCAA的水平（P<0.05，P<0.01）。与APN组比较，APN+CpC组，其细胞内P-AMPK水平显著下降（P<0.01），P-BCKD E1α及上清中BCAA的水平明显上升（分别为P<0.05及P<0.01）。结论： APN可显著促进HepG2细胞BCAA的分解代谢，其作用机制与AMPK磷酸化作用的上调相关。
Abstract:
AIM:To investigate the promoter effect of adiponectin (APN) on the branched-chain amino acid (BCAA) catabolism of HepG2 cells and the underlying mechanism. METHODS: HepG2 cells randomly received a control, APN, APN+CpC and AICAR. Expressions of protein p-AMPK, AMPK, p-BCKD E1α and BCKD E1α were analyzed by Western blot and the BCAA level of supernatant from cultured HepG2 was detected by ELISA. RESULTS: Compared with that of vehicle, the supernatant BCAA level from cultured HepG2 significantly decreased (P<0.01) when APN or AICAR was administered, but the expression of p-AMPK protein from cells increased (P<0.01). Administration of CpC to HepG2 cells increased the supernatant BCAA level (P<0.01), but decreased the p-AMPK expression (P<0.01). CONCLUSION: Adiponectin notably improves the BCAA catabolism of HepG2 cells through upregulating p-AMPK expression.

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备注/Memo

备注/Memo:
收稿日期：2013-11-27.通讯作者：陶凌，主任医师，主要从事糖尿病与心血管保护研究 Email:lingtao2006@gmail.com 作者简介：杜朝升，硕士生 Email:risensun3 @gmail.com
更新日期/Last Update: 2014-05-06