文章摘要

文章信息/Info

Title:: Expression levels of CD44 in surface of peripheral blood mononuclear cells are related to severity of coronary heart disease

作者:: 李真真; 陈德伟; (福建医科大学省立临床医学院心血管内科，福建福州 350001)

Author(s):: LI Zhen-zhen; CHEN De-wei; (Department of Cardiovascular Medicine, Provincial Clinical Medicine College, Fujian Medical University, Fuzhou 350001, Fujian, China)

关键词:: 冠状动脉疾病; 外周血单个核细胞; CD44 mRNA

Keywords:: coronary disease; peripheral blood mononuclear cells; CD44 mRNA

分类号:: R54

DOI:: -

文献标识码:: A

摘要:: 目的:观察不同类型冠心病患者外周血单个核细胞（PBMC）表面CD44的表达变化情况，并探讨其临床意义。方法：采用实时荧光定量RT-PCR法，对正常对照30例，稳定型心绞痛(SAP)26例，不稳定型心绞痛(UAP) 29例、急性心肌梗死(AMI)27例的PBMC表面CD44 mRNA进行检测，并以β-actin作为内参进行半定量评价。结果： ①UAP和AMI组PBMC表面CD44 mRNA水平均显著高于SAP组和正常对照组(P<0.05)；②AMI组PBMC表面CD44 mRNA水平显著高于UAP组(P<0.05)；③SAP组与正常对照组相比，PBMC表面CD44 mRNA水平无统计学差异。结论： PBMC表面CD44表达的水平与冠心病及其病情的严重程度有关联。

Abstract:: AIM:To observe the expression of CD44 on the surface of peripheral blood mononuclear cells (PBMC) in patients with different types of coronary heart disease (CHD) and to investigate its clinical significance. METHODS: Thirty normal subjects, 26 patients with stable angina pectoris (SAP) and 56 patients with acute coronary syndrome (ACS) including 29 with unstable angina pectoris (UAP) and 27 with acute myocardial infarction (AMI) were enrolled in this study. Serum CD44mRNA levels on the surface of PBMC were measured by real-time PT-PCR and were relatively quantified by β-actin as internal standard. RESULTS: The levels of CD44 mRNA in surface of PBMC in UAP patients and AMI patients were both higher than those in patients with SAP and in control group (P<0.05). The levels of CD44 mRNA in surface of PBMC in AMI patients were higher than those in patients with UAP (P<0.05). The levels of CD44 mRNA in surface of PBMC were not significantly different between SAP group and control group. CONCLUSION: The expression levels of CD44 in surface of PBMC were related to the CHD and the severity of the illness.

参考文献/References

[1]Vander Wall AC,Das PK,Bentz van de Berg D,et al.Atherosclerotic lesion in human in stu immunophenocypic analysis suggesting an immune mediated response[J].Lab Invest,1989,61(2):166-170.
[2]Cuff CA,Kothapalli D,Azonobi I,et al.The adhesion receptor CD44 Promotes atherosclerosis by mediating inflammatory cell recruitment and vascular cell activation[J].J Clin Invest,2001,108(7):1031-1040.
[3]Paston RN,Haskard DO,Couther JR,et al.Expression of intercellular adhesion molecule-1 in atherosclerotic plaques ［J］. Am J Pathol, 1992, 140(3):665-673.
[4]Siegelman MH, DeGrendele HC, Estess P. Activation and interaction of CD44 and hyaluronan in immunological systems[J].J Leukoc Biol,1999,66(2):315-321.
[5]Pure E,Cuff CA.A crucial role for CD44 in inflammation[J].Trends Mol Med,2001,7(5):213-221.
[6]Nandi A,Estess P,Siegelman M.Bimolecular complex between rolling and firm adhesion receptors required for cell arrest:CD44 association with VLA-4 in T cell extravasation[J].Immunity,2004,20(4):455-465.
[7]谢谨捷,杨娅.基因敲除鼠在动脉粥样硬化研究中的应用[J].基础医学与临床,2008,28(4):400-403.
[8]Zhao L,Lee E,Zukas AM,et al.CD44 expressed on both bone marrow-derived and non-bone marrow-derived cells promotes atherogenesis in ApoE-deficient mice[J].Arterioscler Thromb Vasc Biol, 2008,28(7):1283-1289.
[9]Berbis P.Hyaluronan in inflammation, auto-immunity and cardio-vascular diseases[J].Ann Dermatol Venereol,2010,137(Suppl 1):S40-S43.
[10]Sadowitz B,Seymour K,Gahtan V,et al.The role of hyaluronic acid in atherosclerosis and intimal hyperplasia[J].J Surg Res,2012,173(2):e63-e72.
[11]Krettek A,Sukhova GK,Schonbeck U,et al.Enhanced expressionof CD44 variants in human atheroma and abdominal aortic aneurysm:possible role for a feedback loop in endothelial cells[J].Am J Pathol,2004,165(5):1571-1581.
[12]安舰,李进,黄剑,等.冠心病人单个核白细胞表面粘附相关蛋白的表达[J].中国动脉硬化杂志,1996,4(1):12-15.

我们的网站为什么显示成这样？

外周血单个核细胞表面CD44表达水平与冠心病及其严重程度有关联

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

文章信息/Info

参考文献/References

备注/Memo

常用功能

导航/Navigate

工具/Tools

统计/Statistics