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|本期目录/Table of Contents|

巴曲酶对实验性犬不稳定型心绞痛作用的研究

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2014年第5期
页码:
533-537
栏目:
基础研究
出版日期:
2014-05-25

文章信息/Info

Title:
Effect of batroxobin on unstable agina pectoris in canine model
作者:
王维亭; 郝春华; 席文恭; 赵专友; 汤立达
(天津市新药安全评价研究中心、天津药物研究院，天津 300193)
Author(s):
WANG Wei-ting;  HAO Chun-hua;  XI Wen-gong;  ZHAO Zhuan-you;  TANG Li-da
(Tianjin Center for New Drug Safety Assessment, Tianjin Institute of Pharmaceutical Research, Tianjin 300193, China)
关键词:
巴曲酶; 不稳定型心绞痛; 冠脉流量降低次数; 犬
Keywords:
batroxobin;  unstable angina pectoris;  cyclic flow reductions;  dog
分类号:
R973,R972.4
DOI:
-
文献标识码:
A
摘要:
目的:研究巴曲酶对实验性不稳定型心绞痛(UAP)的治疗作用。方法： 采用犬冠脉内皮损伤与冠脉狭窄法，制备犬UAP模型，观察巴曲酶对冠脉流量降低次数（CFRs）、血小板(PLT)聚集、纤维蛋白原（FG）及凝血因子（PT、APTT、TT）的影响。实验分为4组，即溶剂对照组、巴曲酶Ⅰ组、巴曲酶Ⅱ组及依替巴肽组，每组5只犬。溶剂对照组给予等体积的生理盐水，巴曲酶Ⅰ、Ⅱ组分别给予0.15和0.3 BU/kg，依替巴肽组给予依替巴肽（0.24 mg/kg）。结果： 给予巴曲酶0.15 BU/kg后，CFRs次数减少，给药后2 h内有效率为80%，且有40%的犬CFRs完全消失，消失时间为(48.5±14.8) min。给予巴曲酶0.3 BU/kg后1、2 h，CFRs次数分别较基础值减少了62.7%和81.0%，与溶剂对照组比较有显著差异(P<0.01)，有效率为100%，且有80%的犬CFRs完全消失，消失时间为(20.7±25.4) min。给予巴曲酶0.3 BU/kg后1 h，对二磷酸腺苷(ADP)诱导的PLT聚集有明显的抑制作用。给予巴曲酶0.15 BU/kg后1、2 h，FG的含量明显降解，与基础值比较降解率分别为51.5%（P<0.05）和57.2%（P<0.05）。给予巴曲酶0.3 BU/kg，降解FG的作用更加明显，给药后1、2 h，与基础值比较降解率分别为68.4%（P<0.01）和78.5%（P<0.01）。给予巴曲酶0.15和0.3 BU/kg后，对PT无明显影响，但可使APTT和TT均明显延长。结论： 静脉给予巴曲酶后，对UAP具有确定的疗效。
Abstract:
AIM:To study the effect of batroxobin on unstable angina pectoris (UAP) in a canine model. METHODS: The left circumflex coronary artery was instrumented with a flow probe, and critical stenosis and arterial wall injury were induced by mechanical clip. Cyclic flow reductions (CFRs) were created in 20 dogs and the efficacy of batroxobin on thrombus formation, antiplatelet aggregation and fibrinogen (FG) degradation was measured. Assessment of prothrombin time (PT), kaolin-activated partial thromboplastin time (APTT) and thrombin time (TT) were observed. Animals were randomly divided into four groups (control group, batroxobin group I, batroxobin group II, eptifibatide group) and each group was comprised of five dogs.The control group was given saline, batroxobin group I and II were given 0.15 and 0.3 BU/kg, respectively. The eptifibatide group was given 0.24 mg/kg. RESULTS: The time of CFRs decreased after batroxobin 0.15 BU/kg administration. The percent of efficacy reached 80% and the percent of absolute inhibition was 40% with (48.5±14.8) min of the time for CFRs absolute disappearance. CFRs decreased by 62.7% and 81.0%, respectively, at 1 h and 2 h time-dependently after batroxobin 0.3 BU/kg administration, with 100% efficacy and 80% absolute inhibition, and the time for CFRs absolute disappearance was (20.7±25.4) min. Batroxobin 0.30 BU/kg inhibited platelet aggregation induced by ADP. FG decreased significantly 1-2 h after batroxobin 0.15 BU/kg administration with degradation of 51.5% and 57.2%, respectively, and batroxobin 0.3 BU/kg produced maximal degragation to about 78.5%. Batroxobin administration exerted no effects on PT but increased APTT and TT. CONCLUSION: Batroxobin has reliable therapeutic efficacy on UAP.

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备注/Memo

备注/Memo:
收稿日期：2013-11-07.
通讯作者：赵专友，研究员，主要从事心脑血管药理学研究 Email：zhaozy@tjipr.com
作者简介：王维亭，副研究员，硕士 Email：23006858@163.com
更新日期/Last Update: 2014-06-05