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|本期目录/Table of Contents|

球囊封堵法建立猪急性心肌梗死模型及MR判定

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2015年第2期
页码:
139-141
栏目:
基础研究
出版日期:
2014-10-25

文章信息/Info

Title:
Establishment by balloon occlusion and MRI identification of porcine model of acute myocardial infarction
作者:
郝跃文; 孙立军
(第四军医大学西京医院放射科，陕西 西安 710032)
Author(s):
HAO Yue-wen;  SUN Li-jun
(Department of Radiology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi, China)
关键词:
急性心肌梗死; 动物模型; 球囊封堵; 磁共振灌注扫描; 小型猪
Keywords:
acute myocardial infarction;  animal model;  balloon occlusion;  magnetic resonance;  myocardial perfusion imaging;  mini-pigs
分类号:
R445.2
DOI:
-
文献标识码:
A
摘要:
目的:运用球囊封堵法建立猪急性心肌梗死(AMI)模型，通过磁共振（MR）判定模型建成与否。方法:实验使用12头小型猪，麻醉后经右侧股动脉将球囊导管置入至左前降支第1对角支下方0.5~1.0 cm处堵闭90 min，直至心电图证实AMI形成，并行MR TrueFisp-PSIR序列心肌延迟灌注扫描及HE染色。结果:12头小型猪中10头成功建立AMI模型，MR延迟灌注扫描在T1WI双反转序列上，室间隔呈半月形低信号。HE染色法显示，封堵血管远端的心肌细胞核淡染、胞浆溶解。结论:冠状动脉球囊堵法是一种简单安全的建立AMI模型的方法，MR可更直观地观察到梗死区面积、组织水肿及心功能改变。
Abstract:
AIM:To establish a porcine model of acute myocardial infarction by balloon occlusion and to assess whether the model was successfully built using MRI. METHODS: Twelve mini-pigs were used in the experiment. After anesthesia, the balloon catheter was positioned at 0.5-1.0 cm below the first diagonal branch of left anterior descending branch and occluded for 90 min until the electrocardiogram confirmed that myocardial infarction occurred. The models underwent myocardial perfusion delay scan with MR TrueFisp-PSIR and HE staining. RERULTS: Ten of 12 mini-pigs underwent successful induction of AMI. In the myocardial perfusion delay scan with T1WI double inverted sequence, ventricular septum presented a half-moon low signal. HE staining showed light dye of distal myocardial nuclei of blocking blood vessels and cytoplasm was dissolved. CONCLUSION: Coronary artery balloon occlusion is a simple and safe approach to establish the myocardial infarction model. MRI can directly display the infarction area, tissue edema and cardiac function changes.

参考文献/References

[1]Hasenfuss G.Animal models of human cardiovascular disease, heart failure and hypertrophy[J].Cardiovasc Res,1998,39(1):60-76.
[2]黄浙勇,葛均波,张少衡,等.猪急性心肌梗死模型发生心室颤动的相关因素分析[J].中国实验动物学报,2007,15(5):351-354.
[3]Ikeda Y,Yutani C,Huang YF,et al.Histological remodeling in an ovine heart failure model resembles human ischemic cardiomyopathy[J].Cardiovasc Pathol,2001,10(1):19-27.
[4]张 晶,傅向华,贾辛未,等.小型猪急性心肌梗死后心力衰竭模型的构建[J].中国实验动物学报,2010,18(1):33-36.
[5]Reek S,Bicknell JL,Walcott GP,et al.Inducibility of sustained ventricular tachycardia in a closed-chest ovine model of myocardial infarction[J].Pacing Clin Electrophysiol,1999,22(4 Pt 1):605-614.
[6]Filippo M,Julsrud P,Araoz P,et al.MRI evaluation of myocardial viability[J]. Radiol Med,2006,111(8):1035-1053.
[7]孙 帅,郭 涛.冠状动脉堵闭法与栓塞法制备小型猪心肌梗死模型的对比[J].中国组织工程研究与临床康复,2009,13(50):9913-9916.
[8]刘 涛,刘正湘,文 莎,等.小型猪急性心肌梗死模型的建立及血流评价[J].心脏杂志,2009,21(3):358-361.
[9]赵新湘,杨达宽,袁曙光,等.延时增强MRI与病理对照评价猪心肌梗死模型失活心肌[J].中国医学影像技术,2009,25(8):1351-1354.

备注/Memo

备注/Memo:
收稿日期：2014-03-16.
基金项目：国家自然科学基金项目资助（81170185）
通讯作者：孙立军，主任医师，主要从事心血管系统疾病影像诊断及基因治疗研究 Email:slj@fmmu.edu.cn
作者简介：郝跃文，住院医师，硕士 Email:1982_edifier@163.com
更新日期/Last Update: 2014-11-18