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|本期目录/Table of Contents|

鸢尾素通过抑制氧化应激减轻高糖/高脂诱导的人脐静脉内皮细胞损伤

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2015年第5期
页码:
506-509
栏目:
基础研究
出版日期:
2015-05-05

文章信息/Info

Title:
Irisin exerts protective effects against high-glucose/high-fat-induced injury of human umbilical vein endothelial cells through alleviating oxidative stress levels
作者:
朱 迪; 张小天; 刘 毅; 辛 超; 张静隆; 王海昌; 陶 凌
（第四军医大学西京医院心内科，陕西 西安 710032）
Author(s):
ZHU Di;  ZHANG Xiao-tian;  LIU Yi;  XIN Chao;  ZHANG Jing-long;  WANG Hai-chang;  TAO Ling
(Department of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi, China)
关键词:
鸢尾素; 人脐静脉内皮细胞; 高糖/高脂; 氧化应激; 凋亡
Keywords:
irisin;  human umbilical vein endothelial cells;  high-glucose/high-fat;  oxidative stress;  apoptosis
分类号:
R543.1
DOI:
-
文献标识码:
A
摘要:
目的 研究鸢尾素（irisin）是否通过抑制氧化应激减轻高糖/高脂所致心肌细胞损伤。方法 将培养的人脐静脉内皮细胞（Human umbilical vein endothelial cells,HUVECs）分为3组：正常对照组、高糖/高脂组及高糖/高脂+irisin组。各组培养24 h后检测细胞存活率、凋亡率、ROS产量、NADPH氧化酶gp91phox表达水平。结果 与正常对照组相比，高糖/高脂组细胞存活率显著下降，ROS产量、NADPH氧化酶gp91phox表达水平、细胞凋亡率显著升高(均P<0.05)，irisin处理可以逆转上述改变（均P<0.05）。结论 高糖/高脂能够增加人脐静脉内皮细胞氧化应激，促进细胞凋亡；irisin可通过减轻氧化应激而发挥内皮保护作用。
Abstract:
AIM To investigate whether irisin exerts endothelial protection in response to high-glucose/high-fat damage through reducing reactive oxygen species (ROS) formation. METHODS Cultured human umbilical vein endothelial cells (HUVECs) were randomly divided into control group (5 mmol/L glucose and 20 mmol/L mannitol), high-glucose/high-fat group (25 mmol/L glucose and 500 μmol/L sodium palmitate, 24 h incubation) and high-glucose/high-fat+irisin group (25 mmol/L glucose, 500 μmol/L sodium palmitate and 1 μg/ml irisin, 24 h incubation). Cells from these groups were harvested for evaluation of cell viability, ROS formation, NADPH oxidase gp91phox expression and apoptosis rate. RESULTS Compared with those in the control group, cell viability decreased, whereas gp91phox expression, ROS formation and apoptosis rate increased in high-glucose/high-fat group (all P<0.05). These changes were all reversed by irisin (all P<0.05). CONCLUSION High-glucose/high-fat leads to oxidative stress in HUVECs and irisin can protect HUVECs by alleviating oxidative stress and endothelial apoptosis.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2014-12-08.
基金项目：国家杰出青年科学基金项目资助（81225001）；国家重点基础研究发展计划项目资助(2013CB531204)；新世纪优秀人才支持计划项目资助（NCET-11-0870）
通讯作者：陶凌，主任医师，主要从事糖尿病心肌损伤研究 Email：lingtao2006@gmail.com
共同通讯作者：王海昌，主任医师，主要从事冠心病防治研究 Email：wanghc@fmmu.edu.cn
作者简介：朱迪，博士生Email：judy3481@163.com
更新日期/Last Update: 2015-04-28