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《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:

2016年第1期

页码:

11-015

栏目:

基础研究

出版日期:

2015-09-15

文章信息/Info

Title:

Correlation between serum BMP4/BMP7 and inflammatory factor distribution and indicators in obese rats

作者:

吴 婷¹; 傅行礼²; 凌秋洋¹; 苏兆亮³; 姜海滨¹; 宗刚军¹

(1.解放军第101医院心血管内科，江苏 无锡 214044；
2.江苏大学医学部教务处，江苏 镇江 212001；
3.镇江市第四人民医院中心实验室，江苏 镇江 212001)

Author(s):

WU Ting¹;  FU Xing-li²;  LING Qiu-yang¹;  SU Zhao-liang³;  JIANG Hai-bin¹;  ZONG Gang-jun¹

(1.Departments of Cardiology, Wuxi Clinical Hospital, Anhui Medical University & PLA 101 Hospital, Wuxi 214044, Jiangsu, China；
2.Dean’s Office Medical Science Center, Jiangsu University, Zhenjiang 212001, Jiangsu, China；
3.Central Laboratory, Zhenjiang Fourth People’s Hospital, Zhenjiang 212001, Jiangsu, China)

关键词:

骨形态发生蛋白-4、7; 肥胖; 炎症; 心血管疾病

Keywords:

BMP4;  BMP7;  obesity;  inflammation;  cardiovascular disease

分类号:

R329

DOI:

-

文献标识码:

A

摘要:

目的 观察肥胖小鼠体内动脉血管与脂肪组织中骨形态发生蛋白-4、7 （BMP4、BMP7）的表达，研究血清BMP4/BMP7比率变化与动脉血管炎症相关性。方法 以ob/ob小鼠为肥胖组，C57BL/6小鼠为正常组，每组各8只。普通饮食喂养12周后，心脏取血并处死，分别取腹主动脉与腹部脂肪组织，用荧光定量PCR和Western blot检测各组织中BMP4、BMP7、IL-1β、IL-9及p-Smad1 mRNA与蛋白的表达。结果 肥胖组血清中BMP4的表达水平显著高于正常组（P<0.05），BMP7的表达两组无明显差异，肥胖组血清BMP4/BMP7比率显著高于正常组（P<0.05）；肥胖组BMP4 mRNA与蛋白在动脉血管的表达高于正常组，在脂肪组织中的表达少于正常组（P<0.05）。BMP7 mRNA与蛋白在动脉血管与脂肪组织中的表达均无显著差异。肥胖组在动脉血管处BMP4mRNA/BMP7mRNA比率显著高于正常组（P<0.05），而在脂肪组织中的表达二者差异不明显。IL-1β和IL-9 mRNA与二者蛋白在动脉血管与脂肪组织中的表达水平均高于正常组（P<0.05）。结论 肥胖小鼠体内动脉血管的炎症反应明显，与血清BMP4/BMP7比率增加相关。

Abstract:

AIM To investigate the expression of BMP4 and BMP7 in arteries and fat tissue and the relationship between arterial vascular inflammation and ratio changes of serum BMP4/BMP7 in obese mice. METHODSEight 4-week-old ob/ob mice were used as the obese group and eight C57BL/6 mice were used as the normal group. After a 12-week full diet, the blood, abdominal aorta and inguinal adipose tissue were collected after all animals were sacrificed and the expressions of protein and mRNA of BMP4, BMP7, IL-1β, IL-9 and p-Smad1 were detected using quantitative PCR and Western blotting. RESULTSSerum expression of BMP4 in the obese group was significantly higher than in the normal group and no significant changes in BMP7 expression were found between groups. Ratio of BMP4/BMP7 in the obese group was significantly higher than in the normal group (P<0.05). Expressions of BMP4 protein and mRNA level in the arteries in obese group were higher than those in the normal group but were lower in adipose tissues in obese group. BMP7 protein and mRNA expressions showed no clear difference in arteries and adipose tissues between groups. Ratio of BMP4 mRNA/BMP7 mRNA showed obvious difference in the arteries between groups (P<0.05), but no significant difference in adipose tissue was observed between groups. Inflammations measured by mRNA and protein expressions of IL-1β and IL-9 in the arteries and obesity in obese group were higher than in the normal group (P<0.05). CONCLUSIONThere is obvious inflammation in the artery of obese mice, which may be related to the increasing ratio of BMP4/BMP7.

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备注/Memo

备注/Memo:

收稿日期：2014-10-14.
>基金项目：国家自然科学基金项目资助(81371657)
通讯作者：宗刚军，副主任医师，主要从事冠心病诊治的研究 Email：zonggj@163.com
作者简介：吴婷，住院医师，硕士生 Email：wutingdoctor@163.com

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更新日期/Last Update: 2015-09-16