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|本期目录/Table of Contents|

大鼠心力衰竭模型的建立与评估

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2016年第6期
页码:
671-675
栏目:
基础研究
出版日期:
2016-07-05

文章信息/Info

Title:
Establishment and assessment of three rat models of heart failure
作者:
李雪健1; 张 晓1; 车运输1; 贾 敏2; 王跃民2; 梁小洁1; 张淑苗2
（第四军医大学：1.学员旅，2.生理学教研室，陕西 西安 710032）
Author(s):
LI Xue-jian1;  ZHANG Xiao1;  CHE Yun-shu1;  JIA Min2;  WANG Yue-min2;  LIANG Xiao-jie1;  ZHANG Shu-miao2
(1.Cadetship Brigade, 2.Department of Physiology, Fourth Military Medical University, Xi’an 710032, Shaanxi, China)
关键词:
心力衰竭; 腹主动脉狭窄; 冠状动脉结扎; 大鼠
Keywords:
heart failure;  abdominal aortic stenosis;  coronary aortic ligation;  rat
分类号:
R332
DOI:
-
文献标识码:
A
摘要:
目的 评估通过腹主动脉狭窄、冠状动脉结扎及腹主动脉狭窄联合冠状动脉结扎3种方法制备大鼠心力衰竭模型的差异。方法 将雄性SD大鼠60只随机分为4组：对照组、腹主动脉狭窄组、冠脉结扎组及腹主动脉狭窄联合冠状动脉结扎组；各组大鼠在术后4周进行心脏超声观察射血分数（EF）、左室短轴缩短率（FS）、左室舒张（LVEDD）和收缩末期内径（LVESD）、左室舒张末期容积（LVEDV）和收缩末期容积（LVESV )；左心室插管观测大鼠心率（HR）、左室收缩末压（LVESP）、左室舒张末压（LVEDP）及左室内压最大上升/下降速率（±dp/dtmax），测量大鼠心脏质量，计算心脏质量/体质量比。结果 对照组和腹主动脉狭窄组4周存活率100%，冠状动脉结扎组4周存活率为86.67%，腹主动脉狭窄联合冠状动脉结扎组4周存活率为13.33%。与对照组相比，腹主动脉狭窄组的EF、FS均明显降低（P<0.05或P<0.01），LVESD、LVESV和LVEDV明显升高（P<0.05或P<0.01），LVEDD和其他心功能指标没有明显变化；冠状动脉结扎组的EF、FS、LVSP和±dp/dtmax均明显下降，LVEDD、LVESD、LVEDV、LVESV和LVEDP明显升高（P<0.01）；除LVEDD外，其余各项指标与腹主动脉狭窄组相比有明显差异（P<0.01）；与对照组相比，腹主动脉狭窄组的心脏质量/体质量比没有明显变化，冠脉结扎组心脏质量/体质量比明显升高（P<0.01）。结论 腹主动脉狭窄法的心衰模型4周时大鼠的EF值仍处于正常范围，心衰症状不明显；冠脉结扎4周制作的心衰模型其心衰程度更为严重，能较好地模拟心衰的临床变化。
Abstract:
AIM To establish and assess the rat models of heart failure using abdominal aortic stenosis, coronary artery ligation or abdominal aortic stenosis combined with coronary artery ligation. METHODSRats were randomly divided into four groups: control, abdominal aortic stenosis (AAS), coronary aortic ligation (CAL) group, and abdominal aortic stenosis combined with coronary aortic ligation (AAS plus CAL). At the fourth week after modeling, echocardiography and hemodynamics were used to assess cardiac functions. After measurement of rat heart weight, heart weight/body weight ratio was calculated. RESULTSAt the fourth week, survival was 100% in the control group and AAS group, 86.67% in the CAL group, and 13.33% in the AAS+CAL group. Ejection fraction (EF) and fractional shortening (FS) were reduced (P<0.05, P<0.01), and LVESD, LVESV and LVEDV were increased in AAS group (P<0.05, P<0.01). No significant changes were found in other parameters. EF, FS, LVSP and ±dp/dtmax were reduced (P<0.01), whereas LVEDD, LVESD, LVEDV, LVESV and LVEDP were all increased in the CAL group (P<0.01), with significant difference between the AAS group and the CAL group in all aforementioned parameters except LVEDD. The heart mass index was increased in the CAL group (P<0.01) and was not significantly changed in the AAS group compared with the control group. CONCLUSIONEF value is in the normal range and symptoms of heart failure in the AAS group are inconspicuous. Heart failure 4 weeks after CAL is more severe and this model can better simulate the clinical changes of heart failure.

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备注/Memo

备注/Memo:
收稿日期：2015-11-11.
基金项目：国家重大新药科技重大专项资金资助（2009ZX09103-671）；国家自然科学基金项目资助（81270402）；陕西省自然科学基础研究计划项目资助（2014JM2-8168）
通讯作者：张淑苗，实验师，博士，主要从事心脏内分泌研究Email：topsniper@126.com
作者简介：李雪健，本科生Email:547215655@qq.com 共同第一作者：张晓，本科生Email:2291465482@qq.com 共同第一作者：车运输，本科生Email:184076058@qq.com
更新日期/Last Update: 2016-07-10