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|本期目录/Table of Contents|

白藜芦醇通过调节自噬延缓ApoE-/-小鼠动脉粥样硬化进展(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2017年第3期
页码:
259-263,275
栏目:
基础研究
出版日期:
2017-01-25

文章信息/Info

Title:
Resveratrol delays the progression of atherosclerosis via inducing autophagy
作者:
段 宇; 王婷婷; 张明明; 胡建强; 蔺 杰; 孙冬冬; 赵志敬
第四军医大学西京医院心内科，陕西 西安 710032
Author(s):
DUAN Yu;  WANG Ting-ting;  ZHANG Ming-ming;  HU Jian-qiang;  LIN Jie;  SUN Dong-dong;  ZHAO Zhi-jing
Department of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi China
关键词:
白藜芦醇; 动脉粥样硬化; 自噬; 巨噬细胞
Keywords:
resveratrol;  atherosclerosis;  autophagy;  macrophage
分类号:
R392.3
DOI:
-
文献标识码:
A
摘要:
目的 探讨白藜芦醇在动脉粥样硬化(AS)进展中的作用和机制。方法 ApoE-/-小鼠60只随机分为4组(每组15只)：对照组、高脂喂养组、白藜芦醇组、高脂喂养+白藜芦醇组。HE染色法观察主动脉斑块病理学改变，生化分析仪检测血脂变化，蛋白质免疫印迹方法(Western blot)测定血管组织微管相关蛋白1轻链3（LC3）以及自噬相关蛋白p62变化。体外培养小鼠巨噬细胞RAW264.7，随机分为4组：对照组、氧化低密度脂蛋白（ox-LDL）损伤组、白藜芦醇组、ox-LDL损伤+白藜芦醇组。TUNEL法检测细胞凋亡，蛋白质Western blot测定自噬相关蛋白LC3Ⅱ、p62的表达。结果 与高脂喂养组比较，白藜芦醇干预后，AS斑块面积减少（P<0.05），LC3Ⅱ水平上调（P<0.05），p62水平下降（P<0.05）,表明白藜芦醇可以诱导自噬；同样，与ox-LDL损伤组比较，白藜芦醇干预后，损伤的巨噬细胞中凋亡水平下降（P<0.05），LC3Ⅱ水平升高（P<0.05），p62水平下降（P<0.05）。结论 AS进展中会伴随自噬水平的下降，给予白藜芦醇处理后，能够延缓AS进展，其机制可能与白藜芦醇调节自噬能力有关。
Abstract:
AIM To investigate the role and mechanism of resveratrol in the progression of atherosclerosis. METHODS Sixty ApoE-/- mice were randomly divided into four groups: control, high-fat diet, resveratrol, high-fat diet+resveratrol. Aortic plaques were observed by hematoxylin-eosin (HE) staining and blood lipids were detected by biochemical analyses. Western blot was performed to detect microtubule-associated protein 1 light chain 3 (LC3) and Protein 62/Sequestosome 1 (p62) changes. Macrophages (murine RAW 264.7 cells) were randomly divided into four groups: control, ox-LDL, resveratrol, ox-LDL+resveratrol. Apoptotic index was examined by TUNEL and expressions of LC3 and p62 were analyzed by Western blot. RESULTS With resveratrol treatment, atherosclerotic plaque area was decreased, LC3 II level increased and p62 level decreased (P<0.05). After ox-LDL cultured RAW264.7 cell treatment with resveratrol, apoptosis in RAW264.7 cells decreased (P<0.05). Similarly, LC3 II level increased and the p62 level declined (P<0.05). CONCLUSION Progression of atherosclerosis is associated with declining autophagy. Resveratrol may delay the progression of atherosclerosis. The mechanism may be related to the enhancement of autophagy.

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备注/Memo

备注/Memo:
收稿日期：2016-08-31.基金项目：国家自然科学基金项目资助(81570318； 81570361) 通讯作者：赵志敬，副教授，主要从事冠心病临床及基础研究 Email:zhaozhj@fmmu.edu.cn 共同通讯作者：孙冬冬，副教授，主要从事冠心病临床及基础研究 Email:wintersun3@gmail.com 作者简介：段宇，硕士生 Email：76793585@qq.com
更新日期/Last Update: 2017-02-20