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Irisin通过激活AMPK促进C2C12细胞葡萄糖摄取

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2017年第5期
页码:
539-543
栏目:
基础研究
出版日期:
2017-03-25

文章信息/Info

Title:
Irisin promotes glucose uptake in C2C12 myocytes by activating AMPK
作者:
李越洋12曹潇方3辛 超2张静隆2朱 迪2陶 凌2刘 毅2
(第四军医大学:1.学员1旅6营24连,2.西京医院心血管内科,陕西 西安 710032;
3宝鸡市疾病预防控制中心,陕西 宝鸡 721000)
Author(s):
LI Yue-yang12 CAO Xiao-fang3 XIN Chao2 ZHANG Jing-long2 ZHU Di2 TAO Ling2 LIU Yi2
(1.24th Company, 6th Battalion, First Cadet Brigade, 2.Department of Cardiology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi, China;
3.Baoji center for disease control and prevention, Baoji 721000, Shaanxi, China)
关键词:
鸢尾素葡萄糖摄取AMPK
Keywords:
Irisin glucose uptake AMPK
分类号:
R589
DOI:
-
文献标识码:
A
摘要:
目的 研究鸢尾素(irisin)对C2C12细胞葡萄糖摄取的影响及机制。方法 将C2C12细胞分为: Control组,不同浓度的irisin组(分为0.1 μg/ml,0.3 μg/ml和1 μg/ml组),高糖/高脂(HG/HF)处理组,HG/HF+不同浓度的irisin组(分为0.1 μg/ml,0.3 μg/ml和1 μg/ml组),Control+Scramble siRNA组,HG/HF+AMPKα2 siRNA组,HG/HF+scramble siRNA组,HG/HF+scramble siRNA+irisin组,HG/HF+AMPKα2 siRNA+irisin组。采用荧光葡萄糖(2-NBDG)检测细胞的葡萄糖摄取;Western blot检测细胞葡萄糖转运体4(GLUT4)转位情况和AMPK磷酸化水平。结果 ①与Control组相比,HG/HF组C2C12细胞葡萄糖摄取降低(P<0.05),不管是否经过高糖处理,Irisin均增加C2C12细胞葡萄糖摄取(P<0.05或P<0.01)。与Control组相比,HG/HF降低鼠骨骼肌细胞膜GLUT4表达(P<0.01),Irisin显著增加HG/HF孵育骨骼肌细胞细胞膜GLUT4表达(P<0.01);②与Control组相比,HG/HF降低C2C12细胞细胞膜AMPK磷酸化水平(P<0.05),在Control组及HG/HF组,Irisin显著增加骨骼肌细胞AMPK的磷酸化水平(P<0.05);③与HG/HF+scramble siRNA+irisin组相比,HG/HF+AMPKα2 siRNA+irisin转染组细胞葡萄糖摄取及细胞表面GLUT4表达均显著减少(P<0.05或P<0.01)。结论 Irisin通过激活AMPK促进小鼠骨骼肌细胞葡萄糖摄取。
Abstract:
AIM To investigate the effect of irisin on glucose uptake in C2C12 myocytes and its underlying mechanism. METHODS C2C12 myocytes were divided randomly into the following groups: Control, irisin (0.1 μg/ml, 0.3 μg/ml, 1 μg/m, respectively), HF/HG, HF/HG+irisin (0.1 μg/ml, 0.3 μg/ml, 1 μg/ml, respectively), Control+scramble siRNA, HF/HG+AMPKα2 siRNA, HF/HG+scramble siRNA, HF/HG+scramble siRNA+irisin, and HF/HG+AMPKα2 siRNA+irisin. The phosphorylated and total AMPK and GLUT4 were determined by Western blot and the uptake of glucose was assayed by analyzing the fluorescence intensity of 2-NBDG. RESULTS irisin significantly increased glucose uptake in C2C12 myocytes in a concentration-dependent fashion both in HF/HG treated group and non-HF/HG treated group (all P<0.05). Compared with those in control group, HF/HG decreased the GLUT4 expression and irisin significantly increased GLUT4 expression (P<0.05). Compared with those in control group, HF/HG decreased the phosphorylated AMPK in C2C12 myocytes and irisin increased the phosphorylated AMPK both in HF/HG treated group and non-HF/HG treated group (P<0.05 or P<0.01). AMPKα2siRNA inhibited the increase of glucose uptake and phosphorylation of AMPK induced by irisin in C2C12 myocytes (P<0.05 or P<0.01). CONCLUSION Irisin increases glucose uptake in myocytes and skeletal muscle by activating AMPK.

参考文献/References

[1]Castillo-Quan JI.From white to brown fat through the PGC-1alpha-dependent myokine irisin:implications for diabetes and obesity[J].Dis Model Mech,2012,5(3):293-295.
[2]Bostr?m P, Wu J,Jedrychowski MP,et al.A PGC1-alpha-dependent myokine that drives brown-fat-like development of white fat and thermogenesis[J].Nature,2012,481(7382):463-468.
[3]Hardie DG,Carling D,Carlson M.The AMP-activated/SNF1 protein kinase subfamily:metabolic sensors of the eukaryotic cell?[J].Annu Rev Biochem, 1998, 67:821-855.
[4]Winder WW, Hardie DG.AMP-activated protein kinase,a metabolic master switch:possible roles in type 2 diabetes[J].Am J Physiol,1999,277,(1 Pt 1):E1-E10.
[5]Koistinen HA,Galuska D,Chibalin AV,et al.5-amino-imidazole carboxamide riboside increases glucose transport and cell-surface GLUT4 content in skeletal muscle from subjects with type 2 diabetes[J].Diabetes,2003,52(5):1066-1072.
[6]Yi W,Sun Y,Gao E,et al.Reduced cardioprotective action of adiponectin in high-fat diet-induced type ii diabetic mice and its underlying mechanisms.Antioxidants & redox signaling[J].Antioxid Redox Signal,2011,15:1779-1788.
[7]Danaei G,Lawes CM,Vander Hoorn S,et al.Global and regional mortality from ischaemic heart disease and stroke attributable to higher-than-optimum blood glucose concentration:comparative risk assessment[J].Lancet,2006,368(9548):1651-1659.
[8]Levey AS,Coresh J.Chronic kidney disease[J].Lancet,2012,379(9811):165-180.
[9]Larsson SC,Mantzoros CS,Wolk A.Diabetes mellitus and risk of breast cancer:a meta-analysis[J].Int J Cancer,2007,121(4):856-862.
[10]Huxley R,Ansary-Moghaddam A,Berrington de González A,et al.Type-II diabetes and pancreatic cancer:a meta-analysis of 36 studies[J].Br J Cancer, 2005,92(11):2076-2083.
[11]Larsson SC,Orsini N,Wolk A.Diabetes mellitus and risk of colorectal cancer:a meta-analysis[J].J Natl Cancer Inst,2005,97(22):1679-1687.
[12]DeFronzo RA,Ferrannini E,Simonson DC.Fasting hyperglycemia in non-insulin-dependent diabetes mellitus:contributions of excessive hepatic glucose production and impaired tissue glucose uptake[J].Metabolism,1989,38(4):387-395.
[13]Gastaldelli A,Baldi S,Pettiti M,et al.Influence of obesity and type 2 diabetes on gluconeogenesis and glucose output in humans: a quantitative study[J].Diabetes,2000,49(8):1367-1373.

备注/Memo

备注/Memo:
收稿日期:2016-06-22.
基金项目:国家自然科学基金项目资助(81400201,81170186,81470478)
通讯作者:刘毅,主治医师,博士,主要从事缺血性心脏病的基础及临床研究Email:liuyimeishan@hotmail.com 共同
通讯作者:陶凌,主任医师,主要从事糖尿病心肌损伤研究 Email:lingtao@ fmmu.edu.cn
作者简介:李越洋,本科生 Email:1018565113@qq.com 共同第一作者:曹潇方,经济师,硕士 Email:1391643423@qq.com
更新日期/Last Update: 2017-04-20