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|本期目录/Table of Contents|

类房室结组织缝隙连接蛋白和离子通道的表达

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2017年第6期
页码:
636-640
栏目:
基础研究
出版日期:
2017-06-01

文章信息/Info

Title:
Connexins and ion channel expression in node like tissues
作者:
吴秋文; 王 帆; 孟 玮; 张 烁
（哈尔滨医科大学附属第二医院心内科，黑龙江 哈尔滨 150001）
Author(s):
WU Qiu-wen;  WANG Fan;  MENG Wei;  ZHANG Shuo
(Department of Cardiology, Second Affiliated Hospital, Harbin Medical University, Harbin 150001, Heilongjiang, China)
关键词:
心脏传导; 房室结; 缝隙连接蛋白; 房室结延伸; 类房室结组织
Keywords:
heart conduction;  atrioventricular node;  connexins;  node extension;  node-like tissues
分类号:
R322.1
DOI:
-
文献标识码:
A
摘要:
目的 明确心脏类房室结组织传导相关的组织学特点，检测其缝隙连接蛋白和离子通道的表达情况，阐述其潜在的电生理意义。方法 对SD大鼠（n=6）的房室结区行连续切片。Masson染色用于显示纤维组织。对影响心脏传导速度的电紧张以及动作电位两个水平的主要蛋白〔缝隙连接蛋白(Cx)43,Cx40,Cx45和离子通道蛋白Nav1.5，Cav3.1，HCN4〕行免疫荧光标记。结果 Masson染色免疫荧光标记显示，后主动脉结、房室结下延伸和致密房室结Cx45、Cav3.1、HCN4表达阳性，Cx43、Cx40表达阴性，Nav1.5表达量显著低于心房（P<0.01）,三者之间Nav1.5表达无组间差异。结论 后主动脉结与房室结下延伸以及致密房室结在电紧张水平和动作电位水平的蛋白表达特点相同。后主动脉结可能和房室结下延伸类似，表现为相同的慢传导特征，可能作为潜在起搏点和某些心律失常的起源点。
Abstract:
AIM To study the histological features of node-like tissues,examine connexins and ion channels expression in the node like tissue,and demonstrate its potential electrophysiological significance in heart conduction. METHODS Adult SD rats (n=6) were used. Serial sections from the entire AVN conduction system to the AV junction were obtained. Masson's trichrome stain was performed on AVN regions to assess fibrous tissue. Three connexins proteins (Cx) including Cx43,Cx40 and Cx45, which dominate the electrotonic conduction of the heart and three main ion channels (Nav1.5,Cav3.1 and HCN4) participating in the depolarization of myocardial cell were immunohistochemically labeled. RESULTS Masson's trichrome stain and immunohistochemical label revealed that retroaortic node (RAN),like compact node (CN) and inferior nodal extensions (INE) had statistically significant higher expression of connexin isoforms and ion channels (including Cx45, Cav3.1 and HCN4) and lower expression of Cx43, Cx40 and Nav1.5). CONCLUSION RAN possesses electrotonic level and action potential level of connexin isoforms and ion channels similar to those of AVN and INE. RAN may have the same conduction properties as INE and could act as an ectopic pacemakers and a source of tachycardia.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2016-11-09.基金项目：黑龙江省自然基金项目资助(Grant D201123)
通讯作者：张烁，主任医师，主要从事病毒性心肌炎及心肌病和心脏起搏与电生理的研究 Email：zhangshuoemail@163.com
作者简介：吴秋文，住院医师，硕士生 Email：489390533@qq.com
更新日期/Last Update: 2017-06-06