超声心动图评价重组Peroxiredoxin-2蛋白对糖尿病心肌病大鼠心功能的影响
《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]
- 期数:
- 2018年第2期
- 页码:
- 146-149
- 栏目:
- 基础研究
- 出版日期:
- 2018-02-15
文章信息/Info
- Title:
- Evaluation the effect of recombinant Peroxiredoxin-2 protein in diabetic cardiomyopathy rats cardiac function by Echocardiography
- Author(s):
- CHEN Ji-ye; SHI Hang; WANG Jun-ping; HUANG Zhen; XIU Jin
- (Department of Electrocardiogram, General Hospital of LiaoHe Oil Field, Panjin 124010, Liaoning, China)
- 关键词:
- 超声心动图; 糖尿病心肌病; 重组过氧化物还原酶-2
- 分类号:
- R540.45
- DOI:
- -
- 文献标识码:
- A
- 摘要:
- 目的 应用超声心动图来检测外源重组过氧化物还原酶(rePrx)-2对糖尿病心肌病(DCM)大鼠心功能的影响。方法 30只SD雄性大鼠分为对照组(n=10)，单纯DCM模型组(单纯模型组，n=10)，干预组(DCM+rePrx-2,n=10只)。干预组于糖尿病模型构建成功后通过腹部皮下植入微渗透泵方法给予rePrx-2蛋白。于DCM大鼠模型建立后第12周检测各组的心脏超声心动图结果，并应用HE及Masson染色对大鼠心肌组织进行病理检查。结果 与单纯模型组相比较，干预组大鼠心脏收缩期室间隔厚度(IVSs)，舒张期室间隔厚度(IVSd)，收缩期左心室内径(LVIDs)，舒张期左心室内径(LVIDd)，舒张期左室后壁厚度(LVPWd)和收缩期左室后壁厚度(LVPWs)显著降低，差异均具有统计学意义(均P<0.05)；同时干预组心功能指标左室射血分数(LVEF)和缩短分数(LVFS)显著增高，差异具有统计学意义(均P<0.05)，Masson及HE染色可见干预组明显改善单纯模型组心肌纤维化损伤程度。结论 通过超声心动图检查，能有效证实rePrx-2对DCM大鼠心肌重构及心功能有明显改善作用。
- Abstract:
- AIM To explore the effect of exogenous recombinant Peroxiredoxin-2 (rePrx-2) in diabetic cardiomyopathy (DCM) rats by Echocardiography. METHODS SD male rats were divided into control group (n=10), DCM model group (n=10), and treatment group (DCM+rePrx-2, n=10). rePrx-2 protein was given by abdominal subcutaneous micro osmotic pump after completion of the DCM model. Echocardiography was used to observe heart structure and function damage of the DCM rat at 12 weeks after DCM model completion. HE and Masson staining were used to observe the pathologic process in the myocardial tissue of DCM rats. RESULTS Compared with the DCM group, rePrx-2 protein significantly decreased cardiac systolic ventricular septal thickness (IVSs), diastolic ventricular septal thickness (IVSd), left ventricular systolic diameter (LVIDs), diastolic left ventricular diameter (LVIDd), diastolic left ventricular posterior wall thickness (LVPWd) and systolic left ventricular posterior wall thickness (LVPWs), (all P<0.05). rePrx-2 protein significantly increased cardiac function indexes of ejection fraction (LVEF) and shortening fraction (LVFS) (all P< 0.05). HE and Masson staining also revealed DCM group myocardial fibrosis damage was improved by rePrx-2 protein. CONCLUSION Echocardiography effectively confirmed the protective role of rePrx- 2 in DCM rats myocardial remodeling and cardiac function.
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备注/Memo
- 备注/Memo:
- 收稿日期：2017-05-31.作者简介：陈继业，主治医师，硕士 Email：xiaoxuetuchenjiye@163.com
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