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IL-1β双相调节血管平滑肌细胞膜电位机制研究

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2018年第4期
页码:
394-399
栏目:
基础研究
出版日期:
2018-04-25

文章信息/Info

Title:
Mechanism of IL-1β biphasic regulation of membrane potential of vascular smooth muscle cells
作者:
肖懿慧; 梁 潇; 李红兵; 高 渊
（西安交通大学附属第一医院心内科，陕西 西安 710061）
Author(s):
XIAO Yi-hui;  LIANG Xiao;  LI Hong-bing;  GAO Yuan
(Department of Cardiology, First Affiliated Hospital, Xi’an Jiaotong University, Xi’an 710061, Shaanxi, China)
关键词:
白介素-1β; BKca通道; H2O2; 大鼠主动脉平滑肌细胞; 膜电位
Keywords:
IL-1β;  the large conductance Ca2+-activated K+ channel;  H2O2;  Rat aortic smooth muscle cells;  membrane potential
分类号:
R392.12
DOI:
-
文献标识码:
A
摘要:
目的 观察不同浓度白介素（IL）-1β对血管平滑肌细胞膜电位的影响，探讨IL-1β通过过氧化氢（H2O2）调节血管平滑肌细胞BKca通道活性的机制。方法 实验分组按照不同浓度分为对照（生理盐水处理）组、IL-1β浓度分别为1 、10 、50 ng/ml组；按照与IL-1β共培养时间分组为30 min 、24 h、48 h组），通过激光共聚焦显微镜检测细胞膜电位探针DIBAC4（3）标记的大鼠主动脉平滑肌细胞（ASMCs）膜电位，观察catalase(H2O2清除剂)、IBTX（BKca通道开放剂）、NS1619（BKca通道阻滞剂）、4-AP（Kv通道阻断剂）对ASMCs膜电位的影响。结果 ①IL-1β对ASMCs膜电位具有双向调节作用，即短时间处理膜电位超极化；长时间处理膜电位去极化，且均有浓度依赖性特征。②IL-1β通过H2O2调节BKca通道，进而影响膜电位；③IL-1β短时间处理ASMCs通过H2O2调节膜电位；长时间处理至少部分通过H2O2调节膜电位。结论 IL-1β对血管平滑肌细胞膜电位存在时间相关的双相调节作用，这一作用可能通过H2O2（短时间）上调BKca通道活性或（长时间）下调BKca通道活性来实现。
Abstract:
AIM To observe the effect of different concentrations of IL-1β on the membrane potential of vascular smooth muscle cells and to investigate the mechanism of IL-1β in regulating large conductance Ca2+-activated K+ channel (BKca) activity in vascular smooth muscle cells by H2O2. METHODS Rat aortic smooth muscle cells (ASMC) were grouped by time and IL-1β concentration. The membrane potential of ASMC labeled with membrane potential probe DIBAC4 (3) was detected by confocal laser scanning microscopy and the effect of IL-1β on ASMC membrane potential was observed when catalase (hydrogen peroxide scavenger), IBTX (BKca channel opener), NS1619 (BKca channel blocker) and 4-AP (Kv channel blocker) were used. RESULTS IL-1β had a bidirectional regulation effect with concentration dependent characteristics on ASMCs membrane potential: short time treatment resulted in membrane potential hyperpolarization and long time treatment resulted in membrane potential depolarization. IL-1β regulated BKca channels via H2O2, thereby affecting membrane potential. ASMCs-treated IL-1β for short time regulated membrane potential by H2O2 and for prolonged time modulated membrane potential, in part, through H2O2. CONCLUSIONThe role of IL-1β in biphasic regulation of membrane potential in vascular smooth muscle cells may be achieved by H2O2 up-regulation of BKca channel activity (short time) or down-regulation of BKca channel activity (for a long time).

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备注/Memo

备注/Memo:
收稿日期：2017-11-30.通讯作者：高渊，主治医师，博士，主要从事冠心病基础研究 Email：ginogao@sohu.com 作者简介：肖懿慧，主治医师，博士 Email：sbw_514@163.com
更新日期/Last Update: 1900-01-01