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高血压病患者动脉平滑肌细胞NO 的变化及其与发病的关系X(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
1998年第2期
页码:
86-88
栏目:
论著
出版日期:
1998-03-01

文章信息/Info

Title:
The changes of nitric oxide in vascular smooth muscle cells of essential hypertension patients and the pathogenesis of essential hypertension
作者:
李宏伟　赵连友　李　雪　李中言
第四军医大学唐都医院心内科 西安 710038
Author(s):
Li Hongwei;  Zhao Lianyou;  Li Xue;  Li Zhongyan
Department of Cardiology, Tangdu Hospital, Fourth Military Medical University, Xi′an 710038
关键词:
高血压　动脉平滑肌细胞　一氧化氮
Keywords:
hypertension　arterial smooth muscle cells　 nitric oxide
分类号:
-
DOI:
-
文献标识码:
-
摘要:
为探讨高血压病(EH) 患者一氧化氮(NO) 浓度变化特点与高血压病发病的关系, 本研究以复合胶元酶法分离培养EH患者和血压正常者(NT) 离体动脉平滑肌细胞(VSMC) , 检测细胞培养液的NO含量和VSMC的一氧化氮合酶(NOS) 活性, 观察EH患者动脉VSMC分泌NO的特点。结果显示: ①EH组VSMC的NO含量和NOS活性均显著低于NT组(P < 0.01) ; ②EH患者和NT VSMC 的NO含量和NOS 性随培养时间延长均显著地增加(P < 0.01) ; ③EH者和NT的NO 含量随NOS活性增强而增加, 并呈显著的正相关(P < 0.01)。表明EH患者VSMC的NOS-NO 系统可能存在功能或结构的异常, 并参与高血压病的发生和发展。
Abstract:
This study was aimed at investigating the changes of nitric oxide (NO) concentration in vitro vascular smooth muscle cells (VSMC) of essential hypertension patients (EH) and the role of these changes in the pathogenesis of hypertension. In our study, the subjects were divided into two groups, the EH group , and the normal contro.group. We seperated and cultured VSMC of the two groups by the compound collagenase enzymemethod. In order to observe the character of EH NO secretion, we examed the NO concentration and the activity of NOS in VSMC. The results showed as follows: ①The NO concentration and the activity of NOS in VSMC of the EH group were significantly lower than those of the control group (P < 0.01) ; ②The NO concentration and the activity of NOS in VSMC of both the groups increased with the prolonged cultivation time; ③The two groups we observed that the concentration of NO was increasing with the increasing activity of enzyme. And there was a positive correlation between the NO concentration and the activity of enzyme. Our study demonstrates that the NOS-NO system of EH is probably abnormal in structure or function and is involved in the activation and development of essential hypertension.

参考文献/References

［1］力武良行, 平田健一, 横山光宏. 血管内皮におけゐNO产生の意义. 综合临床, 1996; 45 (9) : 2078.

［2］Ikeda K, Gutierrez OGJR, Yamorvi Y. Dietay NG-nitroL-arginine induces sutained hypertension in normotensive Wistar-Kyoto rats. Clin Exp Pharmacol Physiol,1992; 19: 583.

［3］王旭开, 杨成明, 刘光耀, 等. 复合胶元酶法分离人血管平滑肌细胞的新方法. 第三军医大学学报, 1993; 15(4) : 357.

［4］Green LC, Wagner DA , Glogowski J , et al. Analysis of nitrate, nitrite, and nitrate in biological fluids. Anal Biochem , 1982; 126: 131.

［5］Feelish M , Noack EA. Correlation between nitric oxide formation during degradation of organic nitrate and activation of guanylate cyclaes. Eur J Pharmacol, 1987; 139(1) : 19.

［6］李中言, 赵连友, 叶季鲜, 等. AVP对大鼠动脉血管平滑肌细胞NO 合成的影响. 中国病理生理杂志, 1996; 12(7) : 742.

［7］Hishikawa K, Nakaki T , Tsuda M , et al. Effect of systemic L-arginine abministration on hemodynamics and nitric oxide release in man. J pn Heart J , 1992; 33 (1) : 44.

备注/Memo

备注/Memo:
(收稿1997-10-22)
更新日期/Last Update: 1998-03-01