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《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:

1999年第2期

页码:

73-76

栏目:

论著

出版日期:

1999-03-01

文章信息/Info

Title:

The effects of CGRP on NOS activity and NO synthesis of VSMCs in essential hypertensive patients

作者:

康建华^XX 赵连友 李 雪 杨学东 乔怀宇

第四军医大学唐都医院心内科　西安 710038

Author(s):

Kang Jianhua^XXXX;  Zhao Lianyou;  Li xue;  Yang xuedong ;  Qiao Huaiyu

Department of Cardiology, Tangdu Hospital, Fourth Military Medical University, Xi’an 710038

关键词:

高血压病　降钙素基因相关肽　一氧化氮　一氧化氮合酶　血管平滑肌细胞

Keywords:

essential hypertension 　　calcitonin gene related peptide　　nitric oxide 　　nitric oxide synthase　　vascular smooth muscle cells

分类号:

-

DOI:

-

文献标识码:

-

摘要:

目的: 为探讨降钙素基因相关肽(CGRP)对高血压病(EH)患者动脉血管平滑肌细胞(VSMC)的一氧化氮合酶(NOS)和一氧化氮(NO)产生的作用。方法: 对EH患者和血压正常者(NT)的动脉VSMC进行培养, 分别测定EH组和NT组VSMC的NO含量和NOS活性, 观察EH患者VSMC NO的产生以及CGRP对其的影响。结果:①基础状态下, EH组VSMC的NO含量和NOS活性分别为42.73±6.76 μmol/(2.5×106cells)和0.24±0.05nmol/(2.5×10⁶cells) , 均显著低于NT组的74.52±4.37μmol/(2.5×10⁶cells)和0.55±0.10 nmol/(2.5×10⁶cells) (P<0.05) ; ②在CGRP作用下, EH组的NO含量和NOS活性分别为88.63 ±4.12 μmol/(2.5×10⁶cells) 和10.55±1.28 nmol/(2.5×10⁶cells) , 均显著低于NT组的100.49±9.69 μmol/(2.5×10⁶cells)和12.45±1.61nmol/(2.5×10⁶cells) (P<0.05) ; ③基础状态和CGRP作用下两组VSMC的NO含量和NOS活性随培养时间延长均显著地增加(P<0.05) ; ④在CGRP作用下, 两组VSMC的NO含量和NOS活性较基础状态下增加和增强非常显著(P<0.01)。结论: EH患者VSMC的NOS活性降低和NO含量减少; CGRP可增强NOS活性, 使NO含量增加。提示: CGRP 含量不足可能是高血压发病的机制之一。

Abstract:

Aim: To investigate the effects of calcitonin gene related peptide(CGRP)on the nitric oxide synthase (NOS) activity and nitric oxide(NO)production of vascular smooth muscle cells (VSMCs)in essential hypertensive (EH)patients.Methods: The VSMCs of EH patients and the normotensives(NT)were cultured.We measured the NO contents and NOS activities of the VSMC in the EH and NT groups separately, observed the NO production of the VSMCs in the EH patients and the effect of CGRP on it.Results: ①Under basal conditions, the NO content and NOS activity of the VSMCs in the EH group were 42.73±6.76 μmol/2.5×10⁶cells and 0.24±0.05nmol/2.5×10⁶cells respectively, which were significantly lower than 74.52±4.37 μmol/2.5×10⁶cells and 0.55±0.10nmol/2.5×10⁶cells of the NT group (P<0.05) ; (2)With the effect of CGRP, the NO content and NOS activity of the EH group were 88.63±4.12 μmol/2.5×10⁶cells and 10.55±1.28 nmol/2.5×10⁶cells respectively, which were remarkably lower than 100.49±9.69 μmol/2.5×10⁶cells and 12.45±1.61nmol/2.5×10⁶cells (P<0.05) of the NT group; ③Under basal conditions and with the effect of CGRP, the NO contents and NOS activities of the two groups significantly increased with the prolongation of culture time (P<0.05) ; (4)With the effect of CGRP, the NO content and NOS activities of both groups increased and enhanced significantly as compared with those under basal conditions (P<0.01).Conclusion: The NOS activity and NO content reduced in VSMC of the EH patients, and CGRP could enhance the activity of NOS and increase the content of NO , suggesting that the deficiency of CGRP might be involved in the pathogenesis of EH.

参考文献/References

［1］Shekhar YC,Anand IS, Sarma R, et al.Effects of prolonged infusion of human Alpha calcitonin gene-related peptide on hemodynamics, renal blood flow and hormone levels in congestive heart failure.Am J Cardiol, 1991; 67: 732.

［2］Prince RG, Gunson DE.Rising interest in nitric oxide synthase.TIBS , 1993; 18 (2) : 35.

［3］Ichiki T, usui M , kato M , et al.Down regulation of angiotensin Ⅱ type1 receptor gene transcription by nitric oxide.Hypertension, 1998; 31 (1p t2) : 342.

［4］ Ikeda K, Gutierrez OGJR, Yamorvi Y.Dietary NG-nitro-L-arginine induces sustained hypertension in normotensive WistarKyoto rats.Clin Exp pharmacol physiol, 1992; 19: 583.

［5］ 李宏伟, 赵连友, 李 雪, 等.高血压病患者动脉平滑肌细胞NO的变化及其与发病的关系.心功能杂志, 1998; 10 (2) : 86.

［6］丁金风, 罗 勇, 刘冬青, 等.降钙素基因相关肽对加压激素的作用.中国循环杂志, 1990; 5 (4) 317.

[7]潘 平, 洪加林, 陈志强.高血压病患者血浆降钙素基因相关肽的观察.温州医学院学报, 1998; 28 (1) : 32.

备注/Memo

备注/Memo:

收稿1998-11-20　修回1999-01-12)X国家自然科学基金资助项目NO.93670317 XX现在武警北京市总队医院（100027）

常用功能

更新日期/Last Update: 1999-03-01