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山莨菪碱对烫在鼠心肌和红细胞的保护作用(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2000年第1期
页码:
4-6
栏目:
论著
出版日期:
2000-01-25

文章信息/Info

Title:
Protective effect of anisodamine on myocardium and erythrocyte in postburn rats
作者:
姚秀娟1; 贾敏1; 秦卫军2; 方正清3
第四军医大学:1药理学教研室 2第四军医大学西京医院 3第四军医大学病理学教研室, 陕西 西安 710032
Author(s):
YAO Xiu-juan 1; JIA Min 1;  QIN Wei-jun 2;  FANG Zheng-qing 3
1Department of Pharmacology, 2Xijing Hospital, 3Department of Pathology, Xi′an 710032, Shaanxi, China
关键词:
山莨菪碱;  烧伤;  心肌;  红细胞;  脂质过氧化作用;  磷脂酶A2
Keywords:
anisodamine;  burns;  myocardium;  erythrocyte;  lipid peroxidation;  phospholipase A 2
分类号:
R972; R331.2
DOI:
-
文献标识码:
A
摘要:
目的 在大鼠 30 %总体表面积 (TBSA) 度烫伤后 ,作者观察了山莨菪碱 (Anisodam ine,Ani)对心肌和红细胞的保护作用。方法 清醒大鼠背部用 80 g· L- 1 硫化钠按 30 % TBSA脱毛 ,2 4h后 ,固定于大鼠烫伤架上 ,脱毛区浸入 10 0℃水中 12 s造成 度烫伤。结果 大鼠烫伤后给予 Ani1,5 ,10 m g· kg- 1 ,ip,能显著降低烫伤后心肌细胞内 CK,L DH释放和心肌组织内丙二醛 (MDA)生成 ,保护心肌内超氧化物歧化酶 (SOD)活性 ,抑制磷脂酶 A2(PL A2 )激活 ,同时 ,明显降低大鼠烫伤后血浆游离血红蛋白浓度 ,抑制红细胞脆性和红细胞异形率的升高 ,减少血浆 MDA浓度, 增加SOD 活性, 抑制PLA 2 活性。结论 Ani 可能通过抗脂质过氧化和降低PLA 2 活性而发挥膜稳定作用, 对烫伤大鼠心肌和红细胞起到保护作用。
Abstract:
AIM Protective effect of anisodamine(Ani) on myocardium and erythrocytein postburn rats. METHODS The rats were inflicted with burn (in 100℃ water 12 s) of Ⅲ degree on 30% total body surface area (TBSA) . RESULTS Ani 1,5,10 mg·kg -1 , ip after burn injury. It was found that Ani significantly decreased the release of CK, LDH and MDA content on myocardium , inhibited PLA 2 activity and increased SOD activity. simultaneity, decreased plasma free haemoglobin content, RBC fragility and erythrocyte abnormal morphologic rate in burned were markedly lowered by Ani, which also discreased the level of serum MDA and enhanced the activity of SOD. CONCLUSION These results indicated that the protective mechanism of Ani on myocardium and erythrocyte after burn injury might be related to membrane stabilization through antilipid peroxidation and PLA2 activity inhibition.

参考文献/References

[1] 陈发明, 杨宗城, 康绍禹, 等. 严重烧伤早期多脏器功能衰竭的动物实验研究[J]. 中华整形烧伤外科杂志, 1992, 8 (1) :16.

[2]　陈意生, 史景泉, 李元平, 等. 家兔严重烧伤后心肌的病理变化[J]. 第三军医大学学报, 1992, 14 (6) : 531.

[3]　苏静怡. 山莨菪碱(65422) 抗休克作用机制的研究进展[J]. 生理科学进展, 1985, 16 (4) : 317.

[4 ]　谢建军, 姚秀娟, 赵德化. 山莨菪碱对大鼠烧伤后血液流变学的影响[J]. 第四军医大学学报, 1997, 18 (1) : 45.

[5]　Yao XJ , Tan YH, Xu ZC, et al. Effects of anisodamine against myocardial ischemic-reperfused injuries and antilipid peroxidation [J]. Acta Pharmacologica Sinica, 1995, 16( 2) :152.

[6]　姚秀娟, 于宝蓉, 谭月华. 山莨菪碱对家兔再灌注心律失常和血流动力学的影响[J]. 第四军医大学学报, 1990, 11 (2) :126.

[7]　Kono Y. Generation of superide radical duging autoxidation of hydroxhamine and assay for superoxide dismatase[J]. Arch Biochem Bioph, 1978, 186 (1) : 189.

[8] Ohkawa H, Ohishi N , Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituhic acid reaction[J]. Snal Biochem , 1979, 95 (2) : 351.

[9]　陈思峰、吴立中. 体液和组织磷脂酶A2简便快速测定法[J].第二军医大学学报, 1989, 10 (3) : 254.

[10] MaractM , Gerbaut L.Improvement of the coomassie blue dye binding method allowing on equal sensitivity to various proteins; application tocerbrospinal flind[J]. Clin Chim Acta, 1982, 122 (1) : 93.

[11] 上海医学化验所. 临床生化检验(上册) [M]. 上海: 上海科学技术出版社, 1979: 123.

[12] 李影林. 临床医学检验手册[M]. 长春: 吉林医学科学技术出版社, 1987: 78.

[13] Sáez JC, W ard PH, Günther B, et al. Superoxide radical involvement in the pathogensis of burn shock [J]. Cir Shock, 1984, 12 (4) : 229.

[14] Chen SF,L i SH, Fei X, et al. Phospholipase A2 induced lung edema and itsmechanisms in isolated perfused guinea pig lung[J]. Inflammation, 1990; 14 (3) : 267.

备注/Memo

备注/Memo:
收稿日期：1999-08-28.
更新日期/Last Update: 2000-01-25