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外源性溶血磷脂酰胆碱和缺血预处理对离体大鼠工作心脏的作用(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2000年第4期
页码:
276-279
栏目:
论著
出版日期:
2000-08-01

文章信息/Info

Title:
The effect of exogenous lysophosphatidylcholine and ischemic preconditioning to isolated working rat hearts
作者:
张春芬1;  伦学庆2;  廉萍3;  李建美1
济宁医学院: 1. 药理学教研室; 2. 附属医院神经外科; 3. 外科总论教研室, 山东济宁272113
Author(s):
ZHANG Chun-fen1;  LUN Xue-qing2;  LIAN Ping3; LI Jian-mei1
1.Department of Pharmacology; 2.Affiliated hospital,Jining Medical College,Jining Shandong 272113,China
关键词:
溶血磷脂酰胆碱; 缺血预处理; 工作心脏; 缺血再灌注损伤
Keywords:
lysophosphatidylcholine; wirking heart; ischemia reperfusion injury; ischemia preconditioning
分类号:
R331.31
DOI:
-
文献标识码:
A
摘要:
目的 观察外源性溶血磷脂酰胆碱(LPC)对离体大鼠工作心脏的损伤作用及缺血预处理(IP)对离体大鼠心脏的保护作用。方法 制备离体大鼠工作心脏模型。随机分为4组 C组 用K-H液连续灌注35 min;Ⅰ组 停灌注40 min,再灌注30 min;L组 用含5 μmol/L LPC的K-H液灌注5 min,再用正常K-H液灌注30 min;P组 停灌注5 min后再灌注5 min,重复3次,然后重复L组全过程。结果 再灌后,L组和I组心功能均明显下降,室颤发生率提高,灌注液中乳酸脱氢酶(LDH)和丙二醛(MDA)含量明显升高,心肌组织中超氧化物歧化酶(SOD)活力明显降低。与L组相比,P组心功能指标明显升高,LDH和MDA含量明显降低,SOD活力较高,无室颤发生。结论 LPC可导致与经典方法类似效果的类缺血再灌注损伤作用,IP对LPC损伤有保护作用。
Abstract:
AIM To observe the ischemia-reperfusion-like effect of exogenous lysophosphatidylcholine(LPC) and the protective effect ischemic preconditioning(IP) in isolated working rat hearts. METHODS Established isolated working rat heart models,and divided into four groups:C group:the hearts were perfused with K-H buffer for 35 min;I group:stop perfusion for 40 min,and then reperfusion for 30 min;L group:the hearts were perfused with K-H buffer containing 5 μmol/L LPC for 5 min, reperfused for 30 min;P group:first,the hearts were in three cycle of 5 min of ischemia and 5 min of reperfusion,and then prefused as the L group. RESULTS Reperfusion in L and I group both caused a cardiac dysfunction and an increase in the release of lactic dehydrogenase (LDH) and malondialdeyde (MDA),and an increase in incidence of ventricular fibrillation (VF),the activity of superoxide dismutase (SOD) were significantly decreased. There is no significant difference between L and I group. IP can improve the recovery of cardiac function and SOD activity,reduced LDH and MDA release and VF incidence. CONCLUSION Exogenous LPC can cause ischemia-reperfusion-like changes,IP has protective effect to LPC injury.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：1999-06-02.
更新日期/Last Update: 2000-08-01