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雌激素受体α在大鼠心脏组织的分布(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2000年第6期
页码:
435-436，439
栏目:
论著
出版日期:
2000-12-01

文章信息/Info

Title:
Distribution of estrogen receptor-α in the cardiac tissues of rats
作者:
高　瞻;  朱妙章;  于　军;  董明清;  郭海涛;  吕顺燕;  臧益民
第四军医大学生理学教研室, 陕西西安710032
Author(s):
GAO Zhan;  ZHU Miao-zhang;  YU Jun;  DONG Ming-qing;  ZANG Yi-min
Department of Physiology, Fourth Military Medical University, Xi'an Shaanxi 710032, China;
关键词:
受体;  雌激素;  心脏;  大鼠;  免疫组织化学
Keywords:
receptor;  estrogen;  heart;  rat;  immunohistochemistr
分类号:
R329.5
DOI:
-
文献标识码:
A
摘要:
目的 观察不同性别 ,不同雌激素水平时雌激素受体α(ERα)在大鼠心脏及培养的心肌和心脏成纤维细胞的分布。方法 通过免疫组织化学方法观察正常雄性及雌性 SD大鼠、卵巢切除大鼠、假手术及雌激素替代治疗大鼠心脏组织 ERα的分布 ,以及雌激素对培养的心肌细胞和心脏成纤维细胞 ERα表达的影响。结果 ERα免疫反应阳性物质呈部位特异性地分布于大鼠心脏组织中;不同性别大鼠未见明显差异;卵巢切除大鼠心脏组织 ERα免疫反应阳性物质明显减少 ;雌激素替代治疗组 ERα的表达恢复至正常水平 ;离体培养的心肌细胞及心脏成纤维细胞 ERα染色阳性 ,给予生理剂量雌激素后 ,心肌及心脏成纤维细胞 ERα阳性染色明显增强。结论 ERα不仅存在于心脏组织中 ,且其表达受雌激素的调控。
Abstract:
AIM To investigate the distribution of ERα in the hearts of different groups of rats. METHODS The distribution of ERα in the cardiac tissues of male and female rats (ovariectomized, estrogen replacement treatment, sham operation and normal) was observed by immunohistochemical method, the effect of estrogen on the expressions of ERα in the cultured cardiomyocytes and cardiac fibroblasts were examined. RESULTS ERα was located in rat hearts in specific pattern and there was no difference in gender. But the staining intensity of ERα in the ovariectomized group was attenuated compared with normal and estrogen replacement treatment groups. 17B-estradiol could enhance the ERα staining in cardiomyocytes and cardiac fibroblasts in vitro. CONCLUSION ERα located broadly in the hearts of rats and its expression was regulated by estrogen.

参考文献/References

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[2] Karas RH, Patterson BL, Mendelsohn ME. Human vascular smooth muscle cells contain functional estrogen receptor [J]. Circulation, 1994, 89 (5): 1943.

[3] Grohe C, Kahlert K, Van EM, et al. Effects of moediprilat on oestrogen-stimulated cardiacfibroblast growth [J]. Br J Pharmacol, 1997, 121 (7): 1350.

[4] Lee HW , Eghbali-webb M. Estrogen enhances proliferative capacity of cardiac fibroblasts by estrogen receptor andmitogen-activated protein kinase-dependent pathways [J]. J Mol Cell Cardiol, 1998, 30 (11) : 1359.

[5] Sicouri S, Antzelech C. Subpopulation of cells with unique electro-physiolgical properties in the deep subepicardium of the canine ventricle the M cell[J]. Circ Res, 1991, 68(6): 1729.

[6] Liu DW , Ant C. Characteristics of the delayed rectifier current (Ikr and Iks) in canine ventricular epicardial, midmyocardial and endocardialmyocytes: A weaker Iks contributes the longer action potential of the M cell[J]. Circ Res, 1995, 76 (3) : 351.

备注/Memo

备注/Memo:
收稿日期：2000-04-13.
更新日期/Last Update: 2000-12-01