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褪黑素对心肌细胞氧化损伤的保护作用(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2001年第3期
页码:
180-182
栏目:
论著
出版日期:
2001-05-01

文章信息/Info

Title:
Antioxidant effects of melatonin on cultured rat cardiomyocytes
作者:
孙　斌1;  李　源1;  郑延松1;  臧益民2;  龚卫琴1
第四军医大学: 1. 西京医院老年病科, 2. 基础部生理学教研室, 陕西西安710032
Author(s):
SUN Bin1;  LI Yuan1;  ZHENG Yan-song1;  ZANG Yi-min2;  GONG Wei-qin1
1.Department of Geriatrics, Xijing Hospital, 2 Department of Physiology, Fourth Military Medical University, Xi'an Shaanxi 710032, China
关键词:
褪黑素;  过氧化氢;  心肌细胞
Keywords:
Melatonin;  hydrogen peroxide;  cardiomyocyte
分类号:
Q576
DOI:
-
文献标识码:
A
摘要:
目的 探讨褪黑素(MT)对过氧化氢(H2O2 )损伤乳鼠心肌细胞的保护作用。方法 胰酶消化法分离培养原代心肌细胞 ,用生化法检测培养液中乳酸脱氢酶 (LDH)浓度,台盼蓝排斥试验检测细胞存活率 ,硫代巴比妥酸法测定细胞中丙二醛 (MDA)含量。结果 10 0 μmol/ L 的 H2 O2 使培养液 L DH漏出量和心肌细胞中 MDA含量明显增加 (分别为 6 .5± 1.6 vs 2 4.8± 1.7U / m l和 1.2 3± 0 .0 5 vs 3.6 5± 0 .2 0 nmol/ 10 6 cells,P<0 .0 1) ,心肌细胞存活率明显下降 (96 .0 %± 1.8% vs 6 0 .0 %± 3.2 % ,P<0 .0 1)。加入 MT后 ,培养液 L DH漏出量和细胞 MDA含量明显降低(分别为 10 .2± 1.3U / ml和 1.5 9± 0 .0 9nm ol/ 10 6 cells) ,细胞存活率明显增高 (90 .0 %± 2 .2 % ) ,与 H2O2 处理组相比均有显著差异 (均 P<0 .0 1)。结论 MT 能对抗过氧化氢所致的心肌细胞损伤, 其保护作用与其抗氧化作用有关。
Abstract:
AIM To investigate the effects of Melatonin (MT) on cardiomyocytes damaged by H2O2. METHODS The rat cardiomyocytes were cultured in DMEM for 4~5 days. Cultured cells were divided into three groups randomly: control group, H 2O 2 group and MT group. The leakage of lactate dehydrogenase (LDH) was assayed by automatic biochemical analyzer. The content of malondialdehyde (MDA) was determined by measuring thiobarbituric acid reactive substances. RESULTS Compared with those of the control group, both LDH leakage and MDA content increased significantly (from 6.5±1.6 U/ml to 24. 8±1.7 U/ml, and from 1.23±0.05 nmol/106 cells to 3. 65±0.20 nmol/106 cells, P<0.01), cell viability decreased significantly (from 96.0% ±1.8% to 60.0% ±3.2%, P<0.01). In MT group, MT inhibited the increase of LDH leakage and MDA content induced by H2O2 (10.2±1.3 U/ml and 1.59±0.09 nmol/106 cells respectively) and increased the cell viability (90.0% ±2.2% ). CONCLUSION MT can protect the cardiomyocytes against H2O2 induced injury with its antioxidative effect.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2000-06-21.
更新日期/Last Update: 2001-05-01