我们的网站为什么显示成这样？

可能因为您的浏览器不支持样式，您可以更新您的浏览器到最新版本，以获取对此功能的支持，访问下面的网站，获取关于浏览器的信息：

|本期目录/Table of Contents|

心肌细胞钙波的影象观察(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2001年第5期
页码:
337-339,342
栏目:
论著
出版日期:
2001-09-01

文章信息/Info

Title:
Observation of the image of calcium waves in cardiac myocytes
作者:
臧伟进1;  于晓江1;  臧益民2;  Christine Lamont3;  Gil Wier3
1. 西安交通大学医学院药理学教研室, 陕西西安710061; 2. 第四军医大学生理学教研室;3. 美国马里兰大学医学院
Author(s):
ZANG Wei-jin1;  YU Xiao-jiang1;  ZANG Yi-min2;  LAMONT Christine3;  WIER Gil3
1.Department of Pharmacology, Xi’an Jiaotong University, Xi’an Shaanxi 710061, China;2.Department of Physiology, Fourth Military Medical University, Xian Shaanxi 710032, China;3.Department of Physiology, University of Maryland at Baltimore,USA
关键词:
心肌细胞;  钙信号;  钙波
Keywords:
cardiac myocytes;  calcium signaling;  calcium wave
分类号:
Q257
DOI:
-
文献标识码:
A
摘要:
目的 观察大鼠心肌细胞内钙波的影象及钙波在心肌细胞内及细胞间的传播。方法 采用激光共聚焦显微镜及细胞内钙荧光染色的观测方法。结果 ①在激光共聚焦显微镜下观察了由钙荧光探针Fluo23 染色的心房肌及心室肌细胞的影象。②观察了心肌细胞内钙在某一区域瞬时性增高的现象即钙波在心肌细胞内传播的动态影象, 钙 波的发生与细胞外钙浓度大小有关。③观察了钙波在心肌细胞与细胞间连接处的传播动态影象。结论 心脏在静息时(尤其高钙负荷时) 出现钙波是钙信号的一种调节机制。钙波具有在细胞内区域性、瞬时性增高, 并较快在细胞内传播, 亦可在细胞间传播。
Abstract:
AIM To investigate the fundamental nature of calcium waves and its propagation inside cells or in cell-cell junctions. METHODS We were studied the calcium waves in rat cardiac myocytes loaded with the fluorescent calcium indicator, fluo-3 using confocal laser microscope.RESULTS ①In the present study, we obtained the images of rat atrial and ventricular myocytes loaded with fluo-3 using confocal laser microscope. ② In the ventricular myocytes, there were local increases of in tracellular calcium , i. e. calcium waves, which could be propagated with in the cells. The occurrence of calcium waves related to extracellular calcium concent ration. ③We also visualized the images of propagation of calcium waves from cell to cell. CONCLUSION In the heart, waves of calcium release ( especially in highly loaded with calcium ) are a normal mechanism of cellular signaling. Calcium wave is a regional increase of calcium transient in side the cells, which can propagated with in the cells, or somehow between the cells.

参考文献/References

[1] 臧伟进, 于晓江, 臧益民. 心肌细胞钙信号研究进展[J]. 生理科学进展, 1999, 30 (2) : 141- 143.

[2] Cheng H, Lederer WJ , Cannell, MB. Calcium sparks:Elementary events underlying excitation-contraction coupling in heart muscle[J]. Science, 1993, 262: 740- 744.

[3] López-López JR, Shacklock PS, Balke CW , et al. Local,stochastic release of Ca2+ in voltage-clamped rat heart cells:Visulization with confocal microscopy[J]. J Physiol, 1994, 480(1) : 21- 29.

[4] López-López JR, Shacklock PS, Balke CW , et al. Localcalcium transients triggered by single L -type calcium channel currents in cardiac cells[J]. Science, 1995, 268: 1042- 1045.

[5] Lipp P, Niggle E. Fundamental calcium release events revealed by two-photon excitation photolysis of caged calcium in guinea-pig cardiac myocytes[J]. J Physiol, 1998, 508 (3) : 801- 809.

[6] Parker I, Zang WJ , Wier WG. Ca2+‘sparks’involving multiple Ca2+ release sites along Z-lines in rat heart cells[J]. J Phyiol,1996, 497 (1) : 31- 38.

[7] Zang WJ , Yu XJ , Boyett MR. Barium block of the muscarinic potassium current in guinea-2pig atrial cells [J]. Pf lügers Arch-Euro J Physiol, 1995, 430: 348- 357.

[8] Shacklock PS, Wier WG, Balke, CW. Local Ca2+ transients(Ca2+ sparks) originate at transverse tubules in rat heart cells[J]. J Physiol, 1995, 487 (3) : 601- 608.

[9] Wier GW. [ Ca2+ ]I transients during excitation-contraction coupling of mammalian heart [A]. Fozzard HA. The Heart and Cardiovascular System [M]. 2 ed. New York: Raven Press,L td , 1992. 1223- 1248.

[10]Cannell MB, Chang H, Lederer WJ. The control of calcium release in heart muscle[J]. Science, 1995, 268: 1045- 1049.

[11] Diaz ME, Trafford AW , O’Neill. Measurement of sarcoplasmic reticulum Ca2+ content and sarcolemmal Ca2+ fluxes in isolated rat ventricular myocytes during spontaneous Ca2+ release[J]. J Physiol, 1997, 501: 3- 16.

备注/Memo

备注/Memo:
收稿日期：2001-01-20.基金项目: 国家自然科学基金资助课题(No. 39970273, 39870334)
更新日期/Last Update: 2001-09-01