我们的网站为什么显示成这样？

可能因为您的浏览器不支持样式，您可以更新您的浏览器到最新版本，以获取对此功能的支持，访问下面的网站，获取关于浏览器的信息：

|本期目录/Table of Contents|

应用胶原膜构建组织工程心脏瓣膜的初步研究(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2001年第5期
页码:
372-376
栏目:
论著
出版日期:
2001-09-01

文章信息/Info

Title:
Primary study of construct tissue engineered heart valve in vitro using collagen membrane
作者:
俞世强;  赵东锷;  汪　钢;  胡巧侠;  刘维永
第四军医大学西京医院心血管外科研究所, 陕西西安710032
Author(s):
YU Shi-qiang ;  ZHAO Dong-e;  WANG Gang ;  HU Qiao-xia; LIU Wei-yong
Institute of Cardiovascular Surgery, Xijing Hospital, Fourth Military Medical University, Xi’an Shaanxi 710032, China
关键词:
胶原膜;  组织工程心脏瓣膜;  生物材料
Keywords:
collagen;  tissue engineered heart valve;  biom aterial
分类号:
R654
DOI:
-
文献标识码:
A
摘要:
目的 探讨应用胶原膜构建组织工程心脏瓣膜的可行性。方法 扫描电镜观察材料结构特点。材料兔皮下包埋实验, 分别于4, 6, 8, 12 周观察材料的生物相容性和降解率。培养犬主动脉瓣间质细胞、主动脉壁间质细胞和皮肤成纤维细胞, 对照其生长曲线、平滑肌A肌动蛋白表达和扫描电镜特点。将犬主动脉壁间质细胞和内皮细胞种植于胶原膜上, 观察其形态并测定细胞合成前列环素的功能。结果 胶原膜呈网孔状结构, 孔径107 Lm。皮下包埋实验显示材料生物相容性好, 体内降解时间为12 周。犬主动脉瓣间质细胞和主动脉壁间质细胞平滑肌A肌动蛋白均为部分阳性表达, 细胞内有大量粗面内质网, 生长曲线相似。种植实验示细胞在材料表面生长良好, 并具有合成、分泌前列环素(84. 2 pg/mL vs 0. 4 pg/mL , P < 0. 05 ) 的功能。结论 以胶原膜为支架体外构建组织工程心脏瓣膜细胞不仅能在材料表面生长, 还能合成、分泌血管活性物质, 是具有“生理功能"的组织工程心脏瓣膜。
Abstract:
AIM To evaluate the feasibility of tissue engineered heart valve on collagen membrane.METHODS Constructive characteristics of collagen membrane were observed by scanning electron microscope. Collagen membrane was implanted subcutaneously in rabbit and extirpated at 4, 6, 8, 10 weeks in order to study its biocompatibility and biodegradibility. Cell growth curve,smooth muscle (SM) α-actin and ultra-microscopic characteristics were compared between canine aortic valve interstitial cells and cells of aortic wall. Cells of aortic wall and aortic endothelium were seeded on the collagen membrane and their function of PGI2 synthesis was determined.RESULTS Porous diameter of collagen membrane was 107 Lm. Collagen mem brane had good biocompatibility and was completely degraded at the end of the 12th week. Both part of interstitial cells from aortic valve and aortic wall expressed SMA-act in, and their growth curves were similar. Cells implanted on collagen membrane grew well and they could synthesize, secret PGI2 (84. 2 pg/mL vs 0. 4pg/mL , P < 0. 05). CONCLUSION It is feasible to construct at issue engineered heart valve in vitro on collagen membrane.

参考文献/References

[1] 朱阳华, 曾　怡, 黄聘和. 生物医学材料应用与免疫反应的关系[J]. 免疫学杂志, 1996, 12 (2) : 136- 140.

[2] Shinoka T, Breuer CK, Tanel KE. Tissue engineering heart valve-leaflet replacement study in a lamb model [J]. Ann Thoracic Surg , 1995, 60 (6 supplI) : I513- I516.

[3] Sodian R, Hoerstrup SP, Sperling JS, et al. Early in vivo experience with tissue-engineered trileaflet heart valves [J].Circulation, 2000, 102 (19 Suppl III ) : III 22- III 29.

[4] Zeltinger J , Landeen LK, Alexander HG, et al. Development and characterization of tissue-engineered aortic valves [J].Tissue Eng ,2001, 7 (1) : 9- 22.

[5] Bairati A ,DeBiasi S. Presence of a smooth muscle system in aortic valve leaflets[J]. Anat Embryol, 1981, 161: 329- 340.

[6] Messier RH, Bass BL , Aly HM , et al. Dural structure and functional phenotypes of the porcine aortic valve interstitial population: Characteristics of the leaflet myiofibroblast [J]. J Surg Res, 1994, 57: 1- 2.

[7] Taylor PM , Allen SP, Yacoub MH. Phenotypic and functional characterization of interstitial cells from human heart valves,pericardium and skin [J]. J Heart Valve Dis, 2000, 9 (1) : 150-158.

备注/Memo

备注/Memo:
收稿日期：2001-04-23.基金项目: 国家自然科学基金资助项目(39970733)
更新日期/Last Update: 2001-09-01