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停搏液中Ca2+ 浓度对未成熟心肌的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2002年第2期
页码:
92-94
栏目:
实验研究
出版日期:
2002-03-01

文章信息/Info

Title:
Effect of calcium content of the cardioplegic solution on immature myocardium
作者:
李　彤;  刘维永;  杨景学;  金　峰;  康进朝
第四军医大学西京医院心血管外科, 陕西 西安 710032
Author(s):
LI Tong ; LIU Wei-yong ;  YANG Jing-xue;  JIN Feng ;  KANG Jin-chao
Department of Cardiovascular Surgery, Xijing Hospital, Xi′an, Shaanxi 710032, China
关键词:
钙离子;  未成熟心肌;  停搏液
Keywords:
calcium;  immature myocardium;  cardioplegia
分类号:
R652.2　
DOI:
-
文献标识码:
A
摘要:
目的 探讨未成熟心肌停搏液中合适的Ca2+ 浓度。方法: 建立未成熟心肌细胞缺氧/复氧损伤模型和未成熟离体心脏缺血/再灌注损伤模型。采用荧光指示剂法测定心肌细胞内游离钙离子浓度和荧光微量法测定细胞内丙二醛(MDA) 含量; 测定离体心脏心功能变化。结果 当心脏停搏液中Ca2+ 浓度为1.2 mmol/L时, 在常温未成熟心肌细胞缺氧/复氧损伤中, 细胞内钙超载、脂质过氧化反应较轻; 而在离体心脏模型中, 心功能恢复比与停搏液中Ca2+浓度的关系呈顶部较平坦的曲线, 以Ca2+ 浓度为1.0mmol/L 时恢复较佳。结论 停搏液中适合未成熟心肌的Ca2+浓度为1.0～1.2mmol/L 。
Abstract:
AIM To study the suitable calcium content of the cardioplegic solution for the immature myocardium. METHODS Models of cultured myocardial cells of neonatal rats and isolated working immature rat hearts were established. In tracellular free Ca2+ contents and MDA levels were measured by mean of fluorescence indicators and function of isolated working immature rat hearts were tested. RESULTS In 1. 2 mmol/L Ca2+ cardioplegic solu ion, only slight reaction of liqid peroxidation occurred and calcium was overloaded in hypoxia and reoxygenation in juries of immature myocardial cells; a bell-shaped, dose-response profole for calcium with optimal protection occurred at 1. 0 mmol/L during function recovery of immature rat hearts. CONCLUSION The optimal calcium concent ration of cardioplegic solution is 1. 0 mmol/L to 1. 2 mmol/L for the immature myocardium.

参考文献/References

[1] Rohinson LA , Harwood DL , Dmaha N. Lowering the calcium concentration in St. Thomas′Hospital Cardioplegic Solution improves protection during hypothermic ischemia [J]. J Thorac Cardiovasc Surg , 1991, 101: 314- 315.

[2] Zweng TN , Iannetoni MD,Bove EL. The concentration of calciumin neonatal cardioplegia[J]. Ann Thorac Surg , 1990, 50: 262- 267.

[3] Baker EJ , Johnt EB. Calcium and cardioplegic protection of the ischemic immature heart [J]. Ann Thorac Surg , 1994, 11 (2) :108- 109.

[4] Teffrey MP, Hillel L ,Davis C. Normocalcemic blood in crystalloid cardioplegia provides better neonatal myocardial protection than dose low-calcium cardiop legia [J]. J Thorac Cardiovasc Surg , 1993, 105: 201- 206.

备注/Memo

备注/Memo:
收稿日期：2001-07-05.
更新日期/Last Update: 2002-03-01