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PDGFR-β反义寡核苷酸对大鼠血管平滑肌细胞凋亡的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2002年第2期
页码:
106-109
栏目:
实验研究
出版日期:
2002-03-01

文章信息/Info

Title:
Effects of PDGFR-B antisense oligonucleotide on apoptosis of rat vascular smooth muscle cells
作者:
顾春虎;  侯英萍;  乔宏庆
第四军医大学西京医院核医学科, 陕西 西安 710032
Author(s):
GU Chun-hu;  HOU Ying-ping ;  QIAO Hong-qing
Xijing Hospital, Forth Military Medical University, Xi,an, Shaanxi 710032, China
关键词:
血小板衍化生长因子;  受体;  再狭窄;  凋亡;  反义寡核苷酸;  平滑肌细胞;  血管
Keywords:
platelet derived growth factor;  receptor;  restenosis;  apoptosis;  antisense oligonucleotide;  smooth muscle cell;  vascular
分类号:
R329.26
DOI:
-
文献标识码:
A
摘要:
目的 探讨血小板衍化生长因子B受体(PDGFR-β) 反义寡核苷酸对培养的大鼠血管平滑肌细胞(VSMC) 凋亡的影响。方法 建立SD 大鼠胸主动脉VSMC体外增殖模型, 取5～10代细胞为实验对象, 按实验目的分为以下几组: ①反义寡核苷酸组; ②正义寡核苷酸组; ③错义寡核苷酸; ④空白对照组。利用透射电镜等观察VSMC加药前后形态学和超微结构的变化, 采用原位末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法(TUNEL) 和流式细胞仪观察和分析PDGFR-β反义寡核苷酸对VSMC凋亡的影响。结果 ①加药后24h细胞形态和超微结构较加药前无明显改变,48h细胞形态和超微结构较加药前有明显改变。②加药后24hTUNEL染色各组未发现凋亡胞,48 h后反义寡核苷酸组细胞爬片染色有较多凋亡阳性细胞出现。③加药24 h 流式细胞仪检测未发现加药组细胞凋亡量与对照组之间有显著差异, 48 h 加药组细胞凋亡量明显高于对照组。结论 PDGFR-β反义寡核苷酸对大鼠VSMC 凋亡有诱导作用。
Abstract:
AIM To study the effects of PDGFR-β antisense oligonucleotides on apoptosis of cultured rat vascular smooth muscle cells(VSMC). METHODS Cultured rat VSMC proliferation model was established in vitro,cells of 5～10 passages were used for the experiment. VSMC were divided into four groups:①control,② PDGFR-β antisense oligonucleotide,③ PDGFR-β sense oligonucleotide,④ PDGFR-β scrambled oligonucleotide. Phase contrast microscope and electron microscope were used to observe the change of cell morphology and cell ultrastructure. Apoptotic VSMC were detected and assayed by terminal deoxynucleotidyl transferase-mediated dUTP nick-enddd labeled(TUNEL) and flow cytometry. RESULTS ①No difference of morphology and ultrastructure was observed between control group and other groups at 24 h. ② Compared with control group,cell morphology and ultrastructure of AODN group varied at 48 h;No apoptotic cell was detected by TUNEL at 24 h. Many apoptotic cells were detected at 48 h by TUNEL in the AODN group.③ There were no difference of apoptosis between control group and AODN group at 24 h. Compared with control group, the numble of apoptotic cells of AODN group was obviously higher at 48 h. CONCLUSION These results suggest that PDGFR-β AODN can induce the apoptosis of cultured VSMC.

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备注/Memo

备注/Memo:
收稿日期：2001-04-25.基金项目: 第四军医大学创新工程项目(CX99A006)　
更新日期/Last Update: 2002-03-01