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|本期目录/Table of Contents|

成年小鼠心肌细胞的分离及电生理特性(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2002年第3期
页码:
210-212
栏目:
实验研究
出版日期:
2002-05-01

文章信息/Info

Title:
Isolation and electrophysiological characteristics of cardiac myocytes in adult mice
作者:
邢　斌;  招明高;  王海芳;  梅其炳
第四军医大学药理学教研室, 陕西 西安 710032
Author(s):
XING Bin;  ZHAO Ming-gao;  WANG Hai-fang ;  MEI Qi-bing
Department of Pharmacology, Fourth Military Medical University, Xi’an, Shaanxi 710032,China
关键词:
小鼠;  心肌细胞;  分离
Keywords:
mice;  cardiac myocytes;  isolation
分类号:
R972.4
DOI:
-
文献标识码:
A
摘要:
目的 摸索成年小鼠心肌细胞分离方法并观察其电生理特性。方法 采用酶消化法分离单个心肌细胞, 应用膜片钳全细胞记录技术记录钙电流和钾电流。结果 本法分离所得心肌细胞横纹清晰, 一次性复钙, 可获得50%～60% 的耐钙细胞; 并具有正常电生理活性, 易于形成高阻封接, 可用于钙电流和钾电流记录。结论 本实验所采用的分离方法经济、简便、成功率高, 所获细胞具有正常的电生理活性。
Abstract:
AIM To grope the mothod used to isolate adult mice cardiac myocytes and observe the electrophysiological characteristics of the myocytes.METHODS Enzyme digestion method was used to isolate single cells and whole-cell patch clamp recording technique was used to record Ca2+and K+currents.RESULTS Rod-shaped, clear-striped cardiac myocytes with normal electrophysiological characteristics were gotten. After exposed to normal calcium concentration solution, 50%~60% calcium-resistant myocytes were left. Giga-ohm seal can be easily achieved and Ca2+and K+currents were successfully recorded.CONCLUSION It is an economic, convenient and reliable method to isolate adult mice cardiac myocytes with normal electrophysiological characteristics.

参考文献/References

[1] Hu B, Mei QB, Yao XJ,et al. A novel contractile phenotype with cardiac transgenic expression of the human P2X4 receptor [J].FASEB J, 2001, 15(14):2739-2741.

[2] Zhou YY, Wang SQ, Zhu WZ,et al. Culture and adenoviral infection of adult mouse cardiac myocytes: methods for cellular genetic physiology[J].Am J Physiol Heart Circ Physiol, 2000,279(1):H429-436.

[3]陈吉球.心肌细胞分离术[J].中国病理生理杂志,1999,15(5):475-477.

[4]商立军,臧益民,王四旺,等.各部位心肌细胞分离技术[J].心功能杂志1999,11(3): 182-184.

[5]刘恭鑫,顾全堡,郭 棋,等.大鼠、豚鼠心肌细胞的简单、快速分离[J].中国应用生理学杂志,1997,13(4):361-362.

备注/Memo

备注/Memo:
收稿日期：2002-02-12.
更新日期/Last Update: 2005-05-01