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血管钠肽抑制肺动脉平滑肌细胞增殖及调控钠尿肽B受体表达的研究(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2002年第4期
页码:
277-280
栏目:
实验研究
出版日期:
2002-07-01

文章信息/Info

Title:
Study of vasonatr in peptide inhibited the proliferation of pulmonary artery smoothmuscle cell and regulated express ionof natriuretic peptide B receptor
作者:
于　军1;  朱妙章1;  董明清1;  高　瞻1;  吕顺艳11;  郭海涛1;  冯华松2
1.第四军医大学生理学教研室, 陕西 西安 710032; 2.海军总医院呼吸科
Author(s):
YU Jun11;  ZHU Miao-zhang1;  DONG Ming-qing1;  GAO Zhan1;  LV Shun-yan1;  GUO Hai-tao1; FENG Hua-song 2
1.Department of Physiology, Fourth Military Medical University, Xi’an, Shaanxi 710032, China; 2.Department of Respiratory Disease, Navy General Hospital
关键词:
血管钠肽;  钠尿肽受体;  肺动脉平滑肌细胞
Keywords:
vasonatrin peptide;  natriuretic peptide receptor;  pulmonary artery smooth muscle cell
分类号:
R33
DOI:
-
文献标识码:
A
摘要:
目的 探讨血管钠肽(VNP) 对肺动脉平滑肌细胞(PASMC) 增殖及钠尿肽B 受体(NPR-B) 基因表达的影响。方法 体外培养大鼠PASMC, 分别用噻唑蓝(MTT) 比色法、总蛋白含量测定、放免分析及打点杂交的方法, 观察了VNP 作用下PASMC 的MTT 吸光值、总蛋白含量、细胞内cGMP、cAMP 浓度和NPR-B 的mRNA 水平。结果 VNP 可以显著抑制血清刺激的PASMC 增殖, 升高细胞内cGMP 浓度及NPR-BmRNA 水平, 但对细胞内cAMP浓度无明显影响。结论 VNP 对PASMC 增殖及NPR-B 基因表达具有调控作用, 并且可能通过cGMP 途径发挥上述作用。
Abstract:
AIM To investigate the effect and mechanism of vasonatrin peptide (VNP) on the proliferation of pulmonary artery smooth muscle cell (PASMC) and gene expression of natriuretic peptide B receptor (NPR-B), METHODS The optical density, protein content, intracellular concentration of cGMP, cAMP and mRNA level of NPR-B were measured by means of MTT colorimetry, protein colorimetry, radioimmunological analysis and dot hybridization respectively. RESULTS VNP significantly inhibited the proliferation of PASMC stimulated by serum,elevated the concentration of cGMP and mRNA level of NPR-B, but had no significant effect on the level of cAMP.CONCLUSION VNP may regulate the proliferation and expression of NPR-B in a cGMP dependent manner.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2001-05-15.基金项目: 国家自然科学基金资助项目( No.39770317, No.39970327)
更新日期/Last Update: 2002-07-01