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|本期目录/Table of Contents|

猪血小板衍生生长因子对NIH3T3细胞DNA合成的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2002年第5期
页码:
372-373, 376
栏目:
基础研究
出版日期:
2002-09-01

文章信息/Info

Title:
Effects of porcine platelet-derived growth factor on DNA synthesis of NIN3T3 cells
作者:
任雨笙1;  崔 芳2;  贾国良3;  樊 民3;  吴宗贵1
第二军医大学长征医院:1.心血管内科,2.理疗科,上海200003;3.第四军医大学西京医院,陕西西安710032
Author(s):
REN Yu-sheng1;  CUI Fang2;  JIA Guo-liang3;  FAN Min3;  WU Zong-gui1
1.Department of Cardiology,Changzheng Hospital,Second Military Medical University, Shang-hai 200003,China
关键词:
血小板衍生生长因子; 氚标记胸腺嘧啶核苷; 致丝裂活性
Keywords:
platelet-derived growth factor; 3H-TdR; mitogenic activity
分类号:
Q813
DOI:
-
文献标识码:
A
摘要:
目的 检测猪血小板衍生生长因子(pPDGF)的致丝裂活性。方法 以NIH3T3细胞作为靶细胞,利用3H-TdR掺入合成DNA的方法,检测pPDGF的致丝裂活性。结果 pPDGF可促进处于静止状态的NIH3T3细胞DNA合成,并呈现出明显的浓度依赖关系,在30 ng/ml的浓度时DNA合成达到高峰,24 h DNA合成最为显著,经2-巯基乙醇处理的pPDGF其致丝裂活性基本丧失。结论 pPDGF具有良好的生物学活性,2-巯基乙醇可使其丧失生物学活性。
Abstract:
AIM To detect the mitogenic activity of porcine platelet-derived growth factor (pPDGF).METHODS In the present experiment,the NIH3T3 cells were cultured and the mitogenic activity of pPDGF were investigated with3H-TdR incorporation in cultured NIH 3T3 cells. RESULTS pPDGF could stimulate DNA synthesis of quiescent NIH 3T3 cells in a concentration range of 1~30 ng/ml dose-dependently,with a maximal response at a concentration of 30 ng/ml at 24 h. The mitogenic activity of pPDGF was destroyed with 2-mercaptoethanol.CONCLUSION pPDGF had a strong mitogenic activity,but lost its biological activity with 2-mercaptoethanol.

参考文献/References

[1] Doanes AM,Irani K,Goldschmidt Clermont PJ,et al. A requirement for racl in the PDGF-stimulated migration of fibroblasts and vascular smooth cells[J].Biochem Mol Biol Int,1998,45 (2):279-287.

[2] 任雨笙,陈 强,贾国良,等.猪血小板衍生生长因子的提取的纯化[J].第二军医大学学报,2000,21(2):246-249.

[3] Stell LL. Luteraction of platelet-activating factor with endothelial and vascular smooth muscle in co-culture[J].J Cell Physical, 1989, 139(2):253-258.

[4] Ruef J,Rao GN,Li F,et al. Induction of rat aortic smooth muscle cell growth by the lipid peroxidation product 4-hydroxy-2-nonenal[J].Circultaion,1998,97(11):1071-1078.

[5] Bokemeyer D, Lindemann M,Kramer HJ. Regulation of mitogen-activated protein kinase phosphatase-1 in vascular smooth muscle cells[J].Hypertension,1998,32(4):661-667.

备注/Memo

备注/Memo:
收稿日期：2001-09-14.基金项目：上海市科技发展基金 (No.014119072)
更新日期/Last Update: 2002-09-01