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兔髂动脉球囊成形及支架术后血管壁超微结构的观察(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2003年第2期
页码:
100-103
栏目:
实验研究
出版日期:
2003-03-01

文章信息/Info

Title:
Experimental study of ultra-microstructure of iliac artery after balloon angioplasty and stent implantation in rabbits
作者:
郭新红; 贾国良; 吕安林; 赵新国; 李飞; 张荣庆
第四军医大学西京医院心血管内科,陕西 西安 71003
Author(s):
GUO Xin-hong; JIA Guo-liang; Lv An-lin; ZHAO Xin-guo; LI Fei; ZHANG Rong-qing
Department of Cardiology,Xi jing Hospital,Fourth Military Medical University,Xi'an,Shaan xi 710032,China
关键词:
冠状动脉疾病; 血管成形术; 经腔; 经皮管状动脉; 再狭窄; 支架; 细胞外基质; 平滑肌细胞
Keywords:
coronary disease; angioplasty; transluminal; percutaneous coronary ; restenosis; extracellular matrix; smooth muscle cell
分类号:
R541.702;R331.3
DOI:
-
文献标识码:
A
摘要:
目的:比较兔髂动脉球囊成形及支架术后血管壁超微结构的变化,探讨支架内再狭窄与球囊血管成形术后再 狭窄的不同机制。方法:10支兔,分别于左髂动脉行NIR支架置入术(S组),右髂动脉行球囊成形术(B组)。术前 3d始予阿司匹林25mg·d-1,直至处死动物。术后皮下注射肝素2500U·d-1,连续7d。术后7d和30d处死动 物。用光镜、透射电镜及免疫组化染色技术观察损伤部位血管壁变化。结果:术后30d光镜检查发现S组新生内膜 平滑肌细胞(SMC)的增生程度明显高于B组(P<0.05)。电镜检查,术后7d,S组较B组可见较多从外膜向中膜及 内膜迁移的肌纤维母细胞,中膜及内膜大量以合成型为主的SMC,细胞外基质(ECM)以胶原及弹性纤维为主。30d 时B组内膜下SMC已转化为收缩型,ECM 以胶原及弹性纤维为主;而S组内膜下大量SMC仍为典型合成型表 现,周围ECM 以氨基聚糖及糖蛋白为主。结论:球囊成形术及支架术后,新生内膜组成成分不同,支架术后新生内 膜增生以SMC增生为主,ECM 以氨基聚糖及糖蛋白为主而球囊成形术后新生内膜增生SMC较少,ECM 以胶原 及弹性纤维为主。
Abstract:
AIM:To investigate the different mechanisms of balloon angioplasty and stent implantation by observing the ultra-microstructure change of iliac artery after balloon angioplasty and stent implantation in rabbits.METHEDS:Balloon angioplasty (groupB) and NIR stent implantation (groupS) were performed in the right and left iliac arteries of the rabbits,respectively.25 mg aspirine,Qd,was given to the rabbits three days before the operation until the animals were executed.2500 U heparin was administered subcutaneously after the operation for 7 days. The animals were executed 7 and 30 days after the peration. The change of the injuredarteries were observed by optical microscope , electron microscope and immunohistochemicaltechnique. RESULTS: Under the optical microscope, 30 days after the operation , SMC proliferation in group S is much more than that of group B (P<0.05). Under the electron microscope,7 days after the operation,more myofibroblasts migrated from adventitia to tunica media and intima in stent iliac artery than inballoon angioplasty iliac artery.Inside the media and intima,there were many synthetic type smooth muscle cells,ECM composed mainly collagen and elastin. At the time of 30 days after operation, most SMCs under the intima of group B had transformed to contractile type,and ECM composed mainly of collagen and elastin.ButSMCs under the intima in group S were still synthetic type, around the SMCs,there was a lot of ECM,which was mainly composed of proteoglycans and glycoproteins. CONCLUTION: The components inside the neointima of group B and group S are different-ECM in the neointima of group B is Mainly collagen and elastin, whereas the ECM in group S mainly consists of proteoglycans and glycoproteins , and there is lots of synthetic type SMCs in the neointima of groupS.

参考文献/References

[1]Topol EJ,Serruys PW.Frontiers in interventional cardiology[J].Circulation,1998,98:1802-1820.

[2]Mehran R,Dangas G,Abizaid AS,et al.Angiographic patterns of in-stent restenosis: classification and implications for long-term outcome[J] . Circulation, 1999, 100: 1872-1878.

[3]Feldman LJ,Aguirre L,Zion M,et al.Interleukin-10 inhibits intimal hyperplasia after angioplasty or stent implantation in hypercholesterolemic rabbits[J].Circulation,2000,101:908-916.

[4]Farb A, Sangiorgi G, Carter AJ, et al.Pathology of acute and chronic coronary stenting in humans[J].Circulation,1999,99:44-52

[5]贾国良,吕安林,王小燕.冠状动脉再狭窄的靶血管局部影响因素[J].第四军医大学学报,2002,23(2):97.

[6]Faggin E,Puato M,Zardo L,et al.Smooth muscle-specific SM22 protein is expressed in the adventitial cells of balloon-injured rabbit carotid artery[ J]. Arterioscler Thromb Vasc Biol,1999,9:1393-1404.

[7]Aikawa M,Rabkin E, Voglic SJ, et al.Lipid lowering promotes accumulation of mature smooth muscle cells expressing smooth muscle myosin heavy chain isoforms in rabbit atheroma [J]. Circ Res,1998,83:1015-1026.

[8]Pauletto P,Puato M,Faggin E,et al.Specific cellular features of atheroma associated with development of neointima after carotid endarterectomy [J]. Circulation, 2000,102(7):771.

[9] Raines EW.The extracellular matrix can regulate vascular cell migration,proliferation,and survival:relationships to vascular disease [J]. Int J Exp Pathol, 2000,81(3):173-182.

[10]Batchelor WB,Robinson R,Strauss BH.The extracelluar matrix in ballon arterial injury:a novel target for restenosis prevention [J]. Prog Cardiovasc Dis,1998,41(1):35-49.

[11]Lafont A, Durand E, Samuel JL, et al. Endothelial dysfunction and collagen accumulation:two independent factors for restenosis and constrictive remodeling after experimental angioplasty[J].Circulation,1999,100(10):1109-1115.

[12] Scott NA,Ross CE,Dunn B,et al.Identification of a potential role for the adventitia in vascular lesion formation after balloon overstretch injury of porcine coronary arteries[J].Circulation,1996,93:2178-2187.

[13] Wilcox JN,Okamato EI,Nakahara KI,et al.Perivascular responses after angioplasty which may contribute to postangioplasty restenosis:a role for circulating myofibroblast precursors?[J].Ann N Y Acad Sci, 2001,947:68-92.

[14]Hoffmann R,Mintz GS.Dussaillant GR,et al.Patterns and mechanisms of in-stent restenosis: a serial intravascular ultrasound study[J]. Circulation, 1996,94:1247-1254.

备注/Memo

备注/Memo:
收稿日期：2002-9-20.
更新日期/Last Update: 2003-03-01