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己酮可可碱对内毒素血症大鼠心肌组织TNF-α基因表达的影响(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2003年第3期
页码:
196-198
栏目:
实验研究
出版日期:
2003-05-01

文章信息/Info

Title:
Effect of pentoxifylline on myocardial TNF-α geneexpression in rats with endotoxemia
作者:
安群星1; 刘利兵1; 程晓东2; 陈健康1; 商立军1; 马文煜3
第四军医大学:1.基础部教学实验中心,2.西京医院检验科,3.基础部微生物学教研室,陕西 西安710032
Author(s):
AN Qun-xing1; LIU Li-bing1; CHENG Xiao-dong2; CHEN Jian-kang1; SHANG Li-jun1; MAWen-yu3
1.Department of Teaching Experiment Center,Faculty of Preclinical Medicine,2.Department of Clinical Laboratories, Xi jing Hospital, 3.Department of Microbiology,Faculty of Preclinical Medicine,Fourth Military Medical University,Xi,an,Shaanxi710032,China
关键词:
己酮可可碱; 内毒素血症; 肿瘤坏死因子-α; 基因表达
Keywords:
pentoxifylline; endotoxemia; tumornecrosisfactor-α; geneexpression
分类号:
Q786
DOI:
-
文献标识码:
A
摘要:
目的:观察己酮可可碱(PTX)对内毒素血症(ETM)大鼠心肌组织肿瘤坏死因子-α(TNF-α)基因表达的影响,探讨PTX对ETM 大鼠心肌组织可能的保护机制。方法:以ETM 大鼠为模型,动物随机分为ETM 模型组(12只)和PTX干预组(12只)。采用逆转录聚合酶链反应(RT-PCR)和放射免疫分析法分别检测不同时间点心肌组织TNF-αmRNA表达水平及血循环TNF-α含量。结果:PTX干预后,心肌组织TNF-α基因表达及血循环TNF-α含量均受到不同程度抑制(P<0.05,P<0.01)。结论:PTX能有效抑制ETM 大鼠心肌TNF-α基因表达,为PTX保护ETM 心肌组织提供了实验依据。
Abstract:
AIM:To investigate the effects of pentoxifylline(PTX) on myocardial gene expression of tumor necrosis factor-α (TNF-α) in rats with endotoxemia(ETM) ,and to evaluate the mechanism underlying PTX on the protection of rats myocardium with ETM.METHODS:ETM models were used in this study.Rats were randomly divided into two groups as follows:ETM model group (n=12), and PTX treatment group (n=12).Reverse transcription polymerase chain reaction and radioimmunoassay methods were used to detect TNF-α gene expression in myocardium and TNF-α levels in plasma at various intervals.RESULTS:TNF-αmRNA expression in myocardium and TNF-α levels in plasma were significantly decreased after the treatment with PTX(P<0.05和P<0.01).CONCLUSION:PTX can effectively inhibit the expression of myocardium TNF-αmRNA in rats with ETM,which may provide the experimental basis for the use of PTX in the treatment of myocardium with ETM.

参考文献/References

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备注/Memo

备注/Memo:
收稿日期：2002-10-25.
更新日期/Last Update: 2003-05-01