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雌激素抑制大鼠心室肌细胞L-型钙电流机制的研究(PDF)

《心脏杂志》[ISSN:1009-7236/CN:61-1268/R]

期数:
2003年第5期
页码:
385-387,431
栏目:
实验研究
出版日期:
2003-09-01

文章信息/Info

Title:
Effects of estrogen on L-type calcium current in rat ventricular myocytes
作者:
高瞻1; 朱妙章1; 周京军1; 陈亚琼2; 高峰1
第四军医大学: 11 生理学教研室, 21 西京医院妇产科, 陕西 西安 710032
Author(s):
GAOZ han1;  ZHU Miao-zhang1;  ZHOU Jing-jun1;  CHEN Ya-qiong2;  GAO Feng1
1.Department of Physiology, 2.Department of Gynecology, Fourth Military Medical University, Xian, Shaanxi 710032, China
关键词:
雌激素; L-型钙电流; 心肌细胞
Keywords:
estrogen;  L-type calcium channel;  myocyte
分类号:
R331.31　
DOI:
-
文献标识码:
A
摘要:
目的:探讨雌激素抑制大鼠心肌细胞L 2型钙电流( ICa,L ) 的可能途径。方法: 通过胶原酶消化得到单个大鼠心室肌细胞, 膜片钳全细胞电压钳方法记录ICa,L。结果: 雌激素(1～ 30 Lmo l?L ) 可剂量依赖性地抑制ICa,L , 雌激素对ICa,L 的抑制作用并不能被雌激素受体的阻断剂Tamoxifen 和IC I 182 780 所阻断。细胞外灌流耦联了牛血清白蛋白(BSA ) 的雌激素[B2est radio l 62(o2carboxy2methyl) oxime: BSA (EST 2BSA ) ], 不能透过细胞膜]也能抑制ICa,L , 但同样浓度的EST 2BSA 细胞内灌流对ICa,L 并没有明显的抑制作用。结论: 雌激素可能是通过膜表面上特异的受体介导了其对L 2型钙电流的抑制作用。
Abstract:
AIM: To explore the possible way involved in the inhibitory effect of estrogen on L-type calcium current (ICa,L). METHODS: Adult rat single ventricular myocytes were obtained enzym etically and ICa, Lw as recorded by using whole"cell patch" clamp technique. RESULTS: Estrogen inhibited ICa, L in a dose-dependent manner and estrogen receptor blocker, IC I 182780 and tamoxifen did not alter the effect. Extracellular perfusion of Bestradio l 6-(o-carboxy-methyl) oxime: BSA (EST-BSA ) could also inhibit I Ca, L , but had no effect on ICa,L by in tracellular perfusion. CONCLUSION: Estrogen could inhibit ICa, L , and the effect may involve a stereo-specif ic binding site on the plasma membrane.

参考文献/References

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[2] Grohe C, Kahlert S, Lobbert K, et al. Expression of oestrogen receptor alpha and beta in rat heart: role of local oestrogen synthesis [J] . J Endocrinol, 1998, 156(2):R1-R7.

[3] Drici MD, Burklow TR, Haridasse V, et al. Sex hormones prolong the QT interval and downregulate potassium channel expression in the rabbit heart [J]. Circulation, 1996, 94:1471-1475.

[4] Nakajima T, Iwasawa K, Oonuma H, et al. Antiarrhythmic effect and its underlying ionic mechanism of 17 beta-estradiol in cardiac myocytes [J]. Br J Pharmacol, 1999, 127(2):429-40.

[5] Okabe K, Inoue Y, Soeda H. Estradiol inhibits Ca2+ and K+ channels in smooth muscle cells from pregnant rat myometrium [J]. Eur J Pharmacol, 1999, 376(122):101- 108.

[6] Tanabe S, Hata T, Hiraoka M. Effects of estrogen on action potential and membrance currents in guinea pig ventricular myocytes [J] . Am J Physiol, 1999, 277(46):H826-H830.

[7] Zhang Y, Song LL , Gu SZ, et al. Inhibitory effects of estradiol on inward rectifier and delayed rectifier K+ currents in guinea pig ventricular myocytes [J] . Acta Pharmacol Sin, 1999, 20(7):631-634.

[8] 周士胜, 臧益民. 介绍一种新的细胞内灌流方法 [J] . 中国病理生理学杂志, 1998, 14(2):116- 117.

[9] Zhai P, Eurell TE, Cooke PS, et al. Myocardial ischemia-reperfusion injury in estrogen receptor-alpha knock out and wild-type mice [J] . Am J Physiol Heart Circ Physiol, 2000, 278(5):H1640-H1647.

[10] Russell KS, Haynes MP, Sinha D, et al. Human vascular endothelial cells contain membrane binding sites for estradio, which mediate rapid intracellular signaling [J]. PNAS , 2000, 97(11):5930- 5935.

备注/Memo

备注/Memo:
收稿日期：2002-12-03.
更新日期/Last Update: 2003-09-01